40个辣椒栽培品种抗番茄斑萎病毒的评价
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摘要
用摩擦接毒的方法,以具有Tsw抗性基因的灯笼椒PI-152225品种为抗性对照,采用ELISA检测法对购自市场的40个辣椒栽培品种抗番茄斑萎病毒(TSWV)的水平进行了评价。结果表明,40个辣椒品种中,白米辣2号对TSWV表现为中抗,12个品种表现为感病,27个品种表现为高感。
Resistances of 40 capsicum cultivars to TSWV from market were evaluated by friction vaccination method and ELISA with cultivar of PI-152225 containing Tsw resistance gene as the control. The results showed that there were one cultivars, Capsicum frutescens cv Baimila No.2 with medium resistance to TSWV, 19 cultivars with susceptibility and 27 with high susceptibility among the 40 cultivars.
Resistances of 40 capsicum cultivars to TSWV from market were evaluated by friction vaccination method and ELISA with cultivar of PI-152225 containing Tsw resistance gene as the control. The results showed that there were one cultivars, Capsicum frutescens cv Baimila No.2 with medium resistance to TSWV, 19 cultivars with susceptibility and 27 with high susceptibility among the 40 cultivars.
引文
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[3]Paliwal YC.Some properties and thrips transmission of tomato spotted wilt virus in Canada[J].Canadian Journal of Botany,1974,52(6):1177-1182.
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[9]郑宽瑜,吴阔,董家红,等.番茄斑萎病毒对云南莴苣类蔬菜的侵染危害[J].植物保护,2015,41(5):174-178.
[10]张仲凯,丁铭,方琦,等.番茄斑萎病毒属(Tospovirus)病毒在云南的发生分布研究初报[J].西南农业学报,2004,17(增):163-168.
[11]张仲凯,方琦,丁铭,等.侵染烟草的番茄斑萎病毒(TSWV)电镜诊断鉴定[J].电子显微学报,2000(3):339-340.
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[13]Boiteux L S.Allelic relationships between genes for resistance to Tomato spotted wilt tospovirus in Capsicum chinense[J].Theoretical and Applied Genetics,1995,90(1):146-149.
[14]Moury B,Palloix A,Gebre-Selassie K,et al.Hypersensitive resistance to Tomato spotted wilt virus in three Capsicum chinense accessions is controlled by a single gene and is overcome by virulent strains[J].Euphytica,1997,94(1):45-52.
[15]Jahn M,Para L,Hoffmann K,et al.Genetic mapping of the Tsw locus for resistance to the tospovirus Tomato spotted wilt virus in Capsicum spp.and its relationship to the Sw-5 gene for resistance to the same pathogen in tomato[J].Molecular Plant-Microbe Interactions,2000,13(6):673-682.
[16]王立浩,张正海,毛胜利,等.甜椒抗番茄斑点萎蔫病毒的种质创新[J].中国蔬菜,2016(2):19-23.
[17]Jiang L,Huang Y,Sun L,et al.Occurrence and diversity of Tomato spotted wilt virus isolates breaking the Tsw resistance gene of Capsicum chinense in Yunnan,southwest China[J].Plant Pathology,2017,66(6):980-989.
[18]Cebolla-Cornejo B J,Soler S,Gomar B,et al.Screening Capsicum germplasm for resistance to Tomato spotted wilt virus(TSWV)[J].Annals of applied biology,2003,143(2)143-152.
[19]秦蕾,梁燕,默宁,等.辣椒种质资源TMV抗性的鉴定与评价[J].中国蔬菜,2017(10):44-50.
[20]陈永对,张洁,吴阔,等.植物源天然产物AHO对番茄斑萎病毒属病毒病的田间防效[J].南方农业学报,2014,45(12):2167-2171.
[21]张洁,陈永对,吴阔,等.6种杀虫剂对烟田番茄斑萎病毒属病毒传毒蓟马的药效试验[J].江苏农业科学,2014(4):95-97.
[2]Pappu H,Jones R,Jain R.Global status of tospovirus epidemics in diverse cropping systems:successes achieved and challenges ahead[J].Virus Research,2009,141:219-236.
[3]Paliwal YC.Some properties and thrips transmission of tomato spotted wilt virus in Canada[J].Canadian Journal of Botany,1974,52(6):1177-1182.
[4]Mumford RA,Barker I,Wood KR.The biology of the tospoviruses[J].Annals of Applied Biology,1996,128:159-183.
[5]Gera A,Kritzman A,Cohen J,et al.Tospoviruses infecting vegetable crops in Israel[J].Eppo Bulletin,2000,30(2):289-292.
[6]Dong J H,Yin Y Y,Xu X Y,et al.First report of Tomato spotted wilt virus in tomato and tobacco in China[J].Journal of Plant Pathology,2010,92(4):S121.
[7]董家红,尹跃艳,徐兴阳,等.番茄斑萎病毒在云南的发生为害[C]//中国植物保护学会.公共植保与绿色防控,北京:中国农业科学技术出版社,2010.
[8]方琦,丁铭,董家红,等.云南澳洲坚果苗木感染番茄斑萎病毒属病毒初报[J].园艺学报,2013,40(2):350-354.
[9]郑宽瑜,吴阔,董家红,等.番茄斑萎病毒对云南莴苣类蔬菜的侵染危害[J].植物保护,2015,41(5):174-178.
[10]张仲凯,丁铭,方琦,等.番茄斑萎病毒属(Tospovirus)病毒在云南的发生分布研究初报[J].西南农业学报,2004,17(增):163-168.
[11]张仲凯,方琦,丁铭,等.侵染烟草的番茄斑萎病毒(TSWV)电镜诊断鉴定[J].电子显微学报,2000(3):339-340.
[12]Boiteux L S,de魣vila A C.Inheritance of a resistance specific to Tomato spotted wilt tospovirus in Capsicum chinense.PI-159236[J].Euphytica,1994,75:139-142.
[13]Boiteux L S.Allelic relationships between genes for resistance to Tomato spotted wilt tospovirus in Capsicum chinense[J].Theoretical and Applied Genetics,1995,90(1):146-149.
[14]Moury B,Palloix A,Gebre-Selassie K,et al.Hypersensitive resistance to Tomato spotted wilt virus in three Capsicum chinense accessions is controlled by a single gene and is overcome by virulent strains[J].Euphytica,1997,94(1):45-52.
[15]Jahn M,Para L,Hoffmann K,et al.Genetic mapping of the Tsw locus for resistance to the tospovirus Tomato spotted wilt virus in Capsicum spp.and its relationship to the Sw-5 gene for resistance to the same pathogen in tomato[J].Molecular Plant-Microbe Interactions,2000,13(6):673-682.
[16]王立浩,张正海,毛胜利,等.甜椒抗番茄斑点萎蔫病毒的种质创新[J].中国蔬菜,2016(2):19-23.
[17]Jiang L,Huang Y,Sun L,et al.Occurrence and diversity of Tomato spotted wilt virus isolates breaking the Tsw resistance gene of Capsicum chinense in Yunnan,southwest China[J].Plant Pathology,2017,66(6):980-989.
[18]Cebolla-Cornejo B J,Soler S,Gomar B,et al.Screening Capsicum germplasm for resistance to Tomato spotted wilt virus(TSWV)[J].Annals of applied biology,2003,143(2)143-152.
[19]秦蕾,梁燕,默宁,等.辣椒种质资源TMV抗性的鉴定与评价[J].中国蔬菜,2017(10):44-50.
[20]陈永对,张洁,吴阔,等.植物源天然产物AHO对番茄斑萎病毒属病毒病的田间防效[J].南方农业学报,2014,45(12):2167-2171.
[21]张洁,陈永对,吴阔,等.6种杀虫剂对烟田番茄斑萎病毒属病毒传毒蓟马的药效试验[J].江苏农业科学,2014(4):95-97.