缺磷胁迫下41%草甘膦异丙铵盐水剂对抗草甘膦大豆农艺性状和生理指标的影响
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摘要
【目的】探明缺磷胁迫下草甘膦对抗草甘膦大豆农艺性状和生理指标的影响,为缺磷条件下抗草甘膦大豆种植时合理使用草甘膦提供理论依据。【方法】以转基因抗草甘膦大豆RR1为材料,采用溶液培养法,在RR1长出真叶时进行缺磷胁迫,第二复叶完全展开时进行4.98 m L/L的41%草甘膦异丙铵盐水剂处理,5 d后测定各生理指标,10 d后测量农艺性状。【结果】正常供磷条件下,草甘膦处理会降低RR1大豆的叶面积、总根长、主根长、地上鲜重、根鲜重、根冠比、根系活力、根系磷含量和叶片磷含量,而株高、叶片SOD活性和MDA含量增加,但与清水处理差异均不显著(P>0.05)。缺磷胁迫下草甘膦处理的RR1大豆的叶面积、总根长、地上鲜重、根系活力、叶片磷含量和根系磷含量降低,而株高、根鲜重、根冠比、叶片SOD活性和MDA含量增加,其中SOD活性变化达显著水平(P<0.05)。【结论】缺磷胁迫下喷施草甘膦,大豆的根鲜重、根冠比、叶片MAD含量和SOD活性增加,叶面积、地上鲜重、根系含磷量和叶片含磷量减少。
【Objective】The effects of glyphosate on agronomic characters and physiological indexes of glyphosate-resistant soybean seedlings under phosphorus deficiency stress were ascertained to provide theoretical references for the rational use of glyphosate in glyphosate-resistant soybean field under phosphorus deficiency stress. 【Method】The glyphosateresistant soybean RR1 was used as the research objective and the solution culture experiment under phosphorus deficiency stress at the true leaf stage was conducted. Glyphosate at 4.98 m L/L was sprayed at the two-trifoliolate leaf stage. Physiological indexes were analyzed 5 days after the glyphosate spraying,and agronomic characters were measured 10 days later.【Result】In normal phosphorus condition,glyphosate decreased the leaf areas,total root length,taproot length,shoot fresh weight,root fresh weight,ratio of root/shoot,and phosphorus content in RR1,but enhanced plant height,SOD activity and MDA content in leaf,however,the differences between glyphosate and water treatment were not significant(P>0.05). The leaf area,total root length,shoot fresh weight,root activity,phosphorus content in leaf,and phosphorus content in root of RR1 treated by 4.98 m L/L glyphosate were decreased under the phosphorus deficiency stress,but the plant height,root fresh weight, ratio of root/shoot,SOD activity and MDA content in leaf were higher than those treated by water under normal phosphorus conditions. And the change of SOD activity was significant(P<0.05). 【Conclusion】Spraying glyphosate under phosphorus deficiency can enhance the root fresh weight,ratio of root/shoot,root fresh weight,MDA content and SOD activity in leaf, but decrease the leaf area,shoot fresh weight,phosphorus content in root and phosphorus content in leaf in glyphosate-resistant soybean seedlings.
【Objective】The effects of glyphosate on agronomic characters and physiological indexes of glyphosate-resistant soybean seedlings under phosphorus deficiency stress were ascertained to provide theoretical references for the rational use of glyphosate in glyphosate-resistant soybean field under phosphorus deficiency stress. 【Method】The glyphosateresistant soybean RR1 was used as the research objective and the solution culture experiment under phosphorus deficiency stress at the true leaf stage was conducted. Glyphosate at 4.98 m L/L was sprayed at the two-trifoliolate leaf stage. Physiological indexes were analyzed 5 days after the glyphosate spraying,and agronomic characters were measured 10 days later.【Result】In normal phosphorus condition,glyphosate decreased the leaf areas,total root length,taproot length,shoot fresh weight,root fresh weight,ratio of root/shoot,and phosphorus content in RR1,but enhanced plant height,SOD activity and MDA content in leaf,however,the differences between glyphosate and water treatment were not significant(P>0.05). The leaf area,total root length,shoot fresh weight,root activity,phosphorus content in leaf,and phosphorus content in root of RR1 treated by 4.98 m L/L glyphosate were decreased under the phosphorus deficiency stress,but the plant height,root fresh weight, ratio of root/shoot,SOD activity and MDA content in leaf were higher than those treated by water under normal phosphorus conditions. And the change of SOD activity was significant(P<0.05). 【Conclusion】Spraying glyphosate under phosphorus deficiency can enhance the root fresh weight,ratio of root/shoot,root fresh weight,MDA content and SOD activity in leaf, but decrease the leaf area,shoot fresh weight,phosphorus content in root and phosphorus content in leaf in glyphosate-resistant soybean seedlings.
引文
敖雪,谢甫绨,张惠君,刘婧琦.2009.磷对不同磷效率基因型大豆保护酶的影响[J].大豆科学,28(1):67-71.Ao X,Xie F T,Zhang H J,Liu J Q.2009.Effect of phosphoruson protective enzymes of soybean cultivars with differentphosphorus efficiencies[J].Soybean Science,28(1):67-71.
蔡柏岩,葛菁萍,祖伟.2008.磷素水平对不同大豆品种叶片及子粒谷氨酰胺合成酶活性的影响[J].植物营养与肥料学报,14(3):592-596.Cai B Y,Ge J P,Zu W.2008.Effect of phosphorus level on glutamine synthetase activities in leaves and seeds of differentsoybean cultivars[J].Plant Nutrition and Fertilizer Science,14(3):592-596.
曹洪玉,李香菊,刘士阳,崔海兰,牛宏波.2011.草甘膦水剂在抗草甘膦转基因大豆田除草效果及安全性研究[J].杂草科学,29(3):22-25.Cao H Y,Li X J,Liu S Y,Cui H L,Niu H B.2011.Study oncontrolling efficiency and safety of glyphosate 41%AS intransgenic glyphosate-resistant soybean field[J].Weed Science,29(3):22-25.
陈俊意,蔡一林,徐莉,杨治国,甘晓玲,潘婷妮.2009.低磷胁迫对玉米叶片色素和形态的影响[J].中国生态农业学报,17(1):125-129.Chen J Y,Cai Y L,Xu L,Yang Z G,Gan X L,Pan T N.2009.Effect of phosphorus stress on the pigment and morphologyof different maize genotypes[J].Chinese Journal of Eco-Agriculture,17(1):125-129.
高俊凤.2006.植物生理学实验指导[M].北京:高等教育出版社.Gao J F.2006.Experimental Guidance for Plant Physiolgy[M].Beijing:Higher Education Press.
黄雪琳,李艳英,董登峰.2009.低磷和铝毒胁迫对大豆活性氧代谢的影响[J].西南农业学报,22(3):615-620.Huang X L,Li Y Y,Dong D F.2009.Comparison of reactiveoxygen metabolism in response to phosphorus deficiencyand aluminum toxicity in two contrasting soybean genotypes[J].Southwest China Journal of Agricultural Sciences,22(3):615-620.
林郑和,陈荣冰,郭少平.2010.植物对缺磷的生理适应机制研究进展[J].作物杂志,(5):5-9.Lin Z H,Chen R B,Guo S P.2010.Research progress on physiological adaptability of plants to phosphorus deficiency[J].Crops,(5):5-9.
刘灵,廖红,王秀荣,严小龙.2008a.不同根构型大豆对低磷的适应性变化及其与磷效率的关系[J].中国农业科学,4(l4):1089-1099.Liu L,Liao H,Wang X R,Yan X L.2008a.Adaptive changes ofsoybean genotypes with different root architectures to lowphosphorus availability as related to phosphorus efficiency[J].Scientia Agricultura Sinica,4(l4):1089-1099.
刘灵,廖红,王秀荣,严小龙.2008b.磷有效性对大豆菌根侵染的调控及其与根构型、磷效率的关系[J].应用生态学报,19(3):564-568.Liu L,Liao H,Wang X R,Yan X L.2008b.Regulation effect ofsoil availability on mycorrhizal infection in relation to rootarchitecture and efficiency of Glycine max[J].Chinese Journal of Applied Ecology,19(3):564-568.
刘鹏,区伟贞,王金祥,严小龙,廖红.2006.磷有效性与植物侧根的发生发育[J].植物生理学通讯,42(3):395-400.Liu P,Ou W Z,Wang J X,Yan X L,Liao H.2006.Phosphorusavailability and lateral root initiation and development ofplants[J].Plant Physiology Communications,42(3):395-400.
苗淑杰,乔云发,韩晓增,王树起,李海波.2009.缺磷对已结瘤大豆生长和固氮功能的影响[J].作物学报,35(7):1344-1349.Miao S J,Qiao Y F,Han X Z,Wang S Q,Li H B.2009.Effectsof phosphorus deficiency on growth and nitrogen fixation ofsoybean after nodule formation[J].Acta Agronomica Sinica,35(7):1344-1349.
任海红,刘学义,李贵全.2008.大豆耐低磷胁迫研究进展[J].分子植物育种,6(2):316-322.Ren H H,Liu X Y,Li G Q.2008.Advances of soybean tolerantto low phosphorus stress[J].Molecular Plant Breeding,6(2):316-322.
王树起,韩晓增,严君,李晓慧,乔云发.2010.缺磷胁迫对大豆根系形态和氮磷吸收积累的影响[J].土壤通报,41(3):644-650.Wang S Q,Han X Z,Yan J,Li X H,Qiao Y F.2010.Impact ofphosphorus deficiency stress on root morphology,nitrogenconcentration and phosphorus accumulation of soybean(Glycine max L.)[J].Chinese Journal of Soil Science,41(3):644-650.
吴俊江,马凤鸣,林浩,刘丽君,钟鹏,林蔚刚,董德建,程莉莉,魏崃,刘德生.2009.不同磷效基因型大豆在生长关键时期根系形态变化的研究[J].大豆科学,28(5):820-824.Wu J J,Ma F M,Lin H,Liu L J,Zhong P,Lin W G,Dong D J,Cheng L L,Wei L,Liu D S.2009.Root morphology traits ofsoybean genotypes with different phosphorus efficiency atimportant growing stages[J].Soybean Science,28(5):820-824.
吴明才,肖昌珍,郑普英.1999.大豆磷素营养研究[J].中国农业科学,32(3):59-65.Wu M C,Xiao C Z,Zheng P Y.1999.Study on the physiologicalfunction of phosphorus to soybean[J].Scientia AgriculturaSinica,32(3):59-65.
原向阳,郭平毅,黄洁,张丽光,郭俊,解丽丽,聂萌恩,王玉国,王宏富.2014.缺磷胁迫下草甘膦对抗草甘膦大豆幼苗光合作用和叶绿素荧光参数的影响[J].植物营养与肥料学报,20(1):221-228.Yuan X Y,Guo P Y,Huang J,Zhang L G,Guo J,Xie L L,NieM E,Wang Y G,Wang H F.2014.Effect of glyphosate onphotosynthesis and chlorophyll fluorescence of leavesof glyphosate-resistant soybean[Glycine max(L.)Merr.]seedlings under phosphorus deficiency stress[J].Plant Nutrition and Fertilizer Science,20(1):221-228.
原向阳,郭平毅,张丽光,王鑫,祁祥,邵冬红.2012.干旱及复水条件下草甘膦对抗草甘膦大豆幼苗渗透调节物质和莽草酸含量的影响[J].核农学报,26(7):1075-1081.Yuan X Y,Guo P Y,Zhang L G,Wang X,Qi X,Shao D H.2012.Effects of glyphosate and post-drought rewatering onosmolytes and shikimic acid content in leaves of glyphosateresistant soybean[Glycine max(L).Merr.]seedlings[J].Journal of Nuclear Agricultural Sciences,26(7):1075-1081.
原向阳,郭平毅,张丽光,王鑫,赵锐,郭秀,宋喜娥.2010.干旱胁迫下草甘膦对抗草甘膦大豆幼苗保护酶活性及脂质过氧化作用的影响[J].中国农业科学,43(4):698-705.Yuan X Y,Guo P Y,Zhang L G,Wang X,Zhao R,Guo X,SongX E.2010.Glyphosate and post-drought rewatering on protective enzyme activities and membrane lipid peroxidationin leaves of glyphosate-resistant soybean[Glycine max(L.)Merr.]seedlings[J].Scientia Agricultura Sinica,43(4):698-705.
原向阳,张丽光,郭平毅,王鑫,姚满生,王宏富.2008.第二复叶期喷施草甘膦对抗草甘膦大豆生理指标及产量构成的影响[J].核农学报,22(1):88-92.Yuan X Y,Zhang L G,Guo P Y,Wang X,Yao M S,Wang H F.2008.Impact of spray glyphosate on physiological indexeand yield composition of glyphosate-resistant soybean attwo-trifoliolate leaf stage[J].Journal of Nuclear Agricultural Sciences,22(1):88-92.
张志良,瞿伟箐.2003.植物生理学实验指导(第3版)[M].北京:高等教育出版社.Zhang Z L,Qu W Q.2003.Experimental Guidance for PlantPhysiology(The Third Edition)[M].Beijing:Higher Education Press.
钟鹏,吴俊江,刘丽君,林蔚刚,董德建,王建丽.2009.低磷和干旱胁迫对不同基因型大豆光合生理特性的影响[J].大豆科学,28(5):806-810.Zhong P,Wu J J,Liu L J,Lin W G,Dong D J,Wang J L.2009.Effects of phosphorus deficiency and drought stress on photosynthetic characters in different genotypic soybeans[J].Soybean Science,28(5):806-810.
Fredeen A L,Raab T K,Rao I M,Terry N.1990.Effects ofphosphorus nutrition on photosynthesis in Glycine max(L.)Merr[.J].Planta,181(3):399-405.
King C A,Purcell L C,Vories E D.2001.Plant growth and nitrogenase activity of glyphosate-tolerant soybean in response to foliar glyphosate applications[J].Agronomy Journal,93(1):179-186.
Reddy K N.2001.Glyphosate-resistant soybean as a weed management tool:opportunities and challenges[J].Weed Biology and Management,1(4):193-202.
Yan X L,Wu P,Ling H Q,Xu G H,Xu F S,Zhang Q F.2006.Plant nutriomics in China:an overview[J].Annals of Botany,98(3):473-482.
Young B G,Young J M,Matthews J L,Owen M D K,Zelaya IA,Hartzler R G,Wax L M,Rorem K W,Bollero G A.2003.Soybean development and yield as affected by three postemergence herbicides[J].Agronomy Journal,95(5):1152-1156.
Zablotowicz R M,Reddy K N.2007.Nitrogenase activity,nitrogen content,and yield responses to glyphosate in glyphosateresistant soybean[J].Crop Protection,26(3):370-376.
蔡柏岩,葛菁萍,祖伟.2008.磷素水平对不同大豆品种叶片及子粒谷氨酰胺合成酶活性的影响[J].植物营养与肥料学报,14(3):592-596.Cai B Y,Ge J P,Zu W.2008.Effect of phosphorus level on glutamine synthetase activities in leaves and seeds of differentsoybean cultivars[J].Plant Nutrition and Fertilizer Science,14(3):592-596.
曹洪玉,李香菊,刘士阳,崔海兰,牛宏波.2011.草甘膦水剂在抗草甘膦转基因大豆田除草效果及安全性研究[J].杂草科学,29(3):22-25.Cao H Y,Li X J,Liu S Y,Cui H L,Niu H B.2011.Study oncontrolling efficiency and safety of glyphosate 41%AS intransgenic glyphosate-resistant soybean field[J].Weed Science,29(3):22-25.
陈俊意,蔡一林,徐莉,杨治国,甘晓玲,潘婷妮.2009.低磷胁迫对玉米叶片色素和形态的影响[J].中国生态农业学报,17(1):125-129.Chen J Y,Cai Y L,Xu L,Yang Z G,Gan X L,Pan T N.2009.Effect of phosphorus stress on the pigment and morphologyof different maize genotypes[J].Chinese Journal of Eco-Agriculture,17(1):125-129.
高俊凤.2006.植物生理学实验指导[M].北京:高等教育出版社.Gao J F.2006.Experimental Guidance for Plant Physiolgy[M].Beijing:Higher Education Press.
黄雪琳,李艳英,董登峰.2009.低磷和铝毒胁迫对大豆活性氧代谢的影响[J].西南农业学报,22(3):615-620.Huang X L,Li Y Y,Dong D F.2009.Comparison of reactiveoxygen metabolism in response to phosphorus deficiencyand aluminum toxicity in two contrasting soybean genotypes[J].Southwest China Journal of Agricultural Sciences,22(3):615-620.
林郑和,陈荣冰,郭少平.2010.植物对缺磷的生理适应机制研究进展[J].作物杂志,(5):5-9.Lin Z H,Chen R B,Guo S P.2010.Research progress on physiological adaptability of plants to phosphorus deficiency[J].Crops,(5):5-9.
刘灵,廖红,王秀荣,严小龙.2008a.不同根构型大豆对低磷的适应性变化及其与磷效率的关系[J].中国农业科学,4(l4):1089-1099.Liu L,Liao H,Wang X R,Yan X L.2008a.Adaptive changes ofsoybean genotypes with different root architectures to lowphosphorus availability as related to phosphorus efficiency[J].Scientia Agricultura Sinica,4(l4):1089-1099.
刘灵,廖红,王秀荣,严小龙.2008b.磷有效性对大豆菌根侵染的调控及其与根构型、磷效率的关系[J].应用生态学报,19(3):564-568.Liu L,Liao H,Wang X R,Yan X L.2008b.Regulation effect ofsoil availability on mycorrhizal infection in relation to rootarchitecture and efficiency of Glycine max[J].Chinese Journal of Applied Ecology,19(3):564-568.
刘鹏,区伟贞,王金祥,严小龙,廖红.2006.磷有效性与植物侧根的发生发育[J].植物生理学通讯,42(3):395-400.Liu P,Ou W Z,Wang J X,Yan X L,Liao H.2006.Phosphorusavailability and lateral root initiation and development ofplants[J].Plant Physiology Communications,42(3):395-400.
苗淑杰,乔云发,韩晓增,王树起,李海波.2009.缺磷对已结瘤大豆生长和固氮功能的影响[J].作物学报,35(7):1344-1349.Miao S J,Qiao Y F,Han X Z,Wang S Q,Li H B.2009.Effectsof phosphorus deficiency on growth and nitrogen fixation ofsoybean after nodule formation[J].Acta Agronomica Sinica,35(7):1344-1349.
任海红,刘学义,李贵全.2008.大豆耐低磷胁迫研究进展[J].分子植物育种,6(2):316-322.Ren H H,Liu X Y,Li G Q.2008.Advances of soybean tolerantto low phosphorus stress[J].Molecular Plant Breeding,6(2):316-322.
王树起,韩晓增,严君,李晓慧,乔云发.2010.缺磷胁迫对大豆根系形态和氮磷吸收积累的影响[J].土壤通报,41(3):644-650.Wang S Q,Han X Z,Yan J,Li X H,Qiao Y F.2010.Impact ofphosphorus deficiency stress on root morphology,nitrogenconcentration and phosphorus accumulation of soybean(Glycine max L.)[J].Chinese Journal of Soil Science,41(3):644-650.
吴俊江,马凤鸣,林浩,刘丽君,钟鹏,林蔚刚,董德建,程莉莉,魏崃,刘德生.2009.不同磷效基因型大豆在生长关键时期根系形态变化的研究[J].大豆科学,28(5):820-824.Wu J J,Ma F M,Lin H,Liu L J,Zhong P,Lin W G,Dong D J,Cheng L L,Wei L,Liu D S.2009.Root morphology traits ofsoybean genotypes with different phosphorus efficiency atimportant growing stages[J].Soybean Science,28(5):820-824.
吴明才,肖昌珍,郑普英.1999.大豆磷素营养研究[J].中国农业科学,32(3):59-65.Wu M C,Xiao C Z,Zheng P Y.1999.Study on the physiologicalfunction of phosphorus to soybean[J].Scientia AgriculturaSinica,32(3):59-65.
原向阳,郭平毅,黄洁,张丽光,郭俊,解丽丽,聂萌恩,王玉国,王宏富.2014.缺磷胁迫下草甘膦对抗草甘膦大豆幼苗光合作用和叶绿素荧光参数的影响[J].植物营养与肥料学报,20(1):221-228.Yuan X Y,Guo P Y,Huang J,Zhang L G,Guo J,Xie L L,NieM E,Wang Y G,Wang H F.2014.Effect of glyphosate onphotosynthesis and chlorophyll fluorescence of leavesof glyphosate-resistant soybean[Glycine max(L.)Merr.]seedlings under phosphorus deficiency stress[J].Plant Nutrition and Fertilizer Science,20(1):221-228.
原向阳,郭平毅,张丽光,王鑫,祁祥,邵冬红.2012.干旱及复水条件下草甘膦对抗草甘膦大豆幼苗渗透调节物质和莽草酸含量的影响[J].核农学报,26(7):1075-1081.Yuan X Y,Guo P Y,Zhang L G,Wang X,Qi X,Shao D H.2012.Effects of glyphosate and post-drought rewatering onosmolytes and shikimic acid content in leaves of glyphosateresistant soybean[Glycine max(L).Merr.]seedlings[J].Journal of Nuclear Agricultural Sciences,26(7):1075-1081.
原向阳,郭平毅,张丽光,王鑫,赵锐,郭秀,宋喜娥.2010.干旱胁迫下草甘膦对抗草甘膦大豆幼苗保护酶活性及脂质过氧化作用的影响[J].中国农业科学,43(4):698-705.Yuan X Y,Guo P Y,Zhang L G,Wang X,Zhao R,Guo X,SongX E.2010.Glyphosate and post-drought rewatering on protective enzyme activities and membrane lipid peroxidationin leaves of glyphosate-resistant soybean[Glycine max(L.)Merr.]seedlings[J].Scientia Agricultura Sinica,43(4):698-705.
原向阳,张丽光,郭平毅,王鑫,姚满生,王宏富.2008.第二复叶期喷施草甘膦对抗草甘膦大豆生理指标及产量构成的影响[J].核农学报,22(1):88-92.Yuan X Y,Zhang L G,Guo P Y,Wang X,Yao M S,Wang H F.2008.Impact of spray glyphosate on physiological indexeand yield composition of glyphosate-resistant soybean attwo-trifoliolate leaf stage[J].Journal of Nuclear Agricultural Sciences,22(1):88-92.
张志良,瞿伟箐.2003.植物生理学实验指导(第3版)[M].北京:高等教育出版社.Zhang Z L,Qu W Q.2003.Experimental Guidance for PlantPhysiology(The Third Edition)[M].Beijing:Higher Education Press.
钟鹏,吴俊江,刘丽君,林蔚刚,董德建,王建丽.2009.低磷和干旱胁迫对不同基因型大豆光合生理特性的影响[J].大豆科学,28(5):806-810.Zhong P,Wu J J,Liu L J,Lin W G,Dong D J,Wang J L.2009.Effects of phosphorus deficiency and drought stress on photosynthetic characters in different genotypic soybeans[J].Soybean Science,28(5):806-810.
Fredeen A L,Raab T K,Rao I M,Terry N.1990.Effects ofphosphorus nutrition on photosynthesis in Glycine max(L.)Merr[.J].Planta,181(3):399-405.
King C A,Purcell L C,Vories E D.2001.Plant growth and nitrogenase activity of glyphosate-tolerant soybean in response to foliar glyphosate applications[J].Agronomy Journal,93(1):179-186.
Reddy K N.2001.Glyphosate-resistant soybean as a weed management tool:opportunities and challenges[J].Weed Biology and Management,1(4):193-202.
Yan X L,Wu P,Ling H Q,Xu G H,Xu F S,Zhang Q F.2006.Plant nutriomics in China:an overview[J].Annals of Botany,98(3):473-482.
Young B G,Young J M,Matthews J L,Owen M D K,Zelaya IA,Hartzler R G,Wax L M,Rorem K W,Bollero G A.2003.Soybean development and yield as affected by three postemergence herbicides[J].Agronomy Journal,95(5):1152-1156.
Zablotowicz R M,Reddy K N.2007.Nitrogenase activity,nitrogen content,and yield responses to glyphosate in glyphosateresistant soybean[J].Crop Protection,26(3):370-376.