中华婪步甲共生放线菌的抗菌活性筛选及菌株BJ37的初步鉴定
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摘要
利用平板分离法从中华婪步甲(Harpalus sinicus)中分离得到56株共生放线菌。其中菌株BJ37具有较好的抗菌活性,其发酵液对枯草芽胞杆菌和白色念珠菌的抑菌圈直径均大于30.0 mm。进一步研究BJ37发酵液不同极性溶剂萃取物的抗菌活性,结果表明在中等极性部位存在活性物质。通过形态学特征和分子生物学分析确定该菌株相似菌株为委内瑞拉链霉菌(Streptomyces venezuelae)。菌株BJ37作为微生物杀菌剂潜在产生菌值得进一步研究。
Fifty-six symbiotic actinomycetes strains were isolated from Harpalus sinicus by plate separation method.Among them strain BJ37 showed the fine antimicrobial activity against Bacillus subtilis and Candida albicans with the inhibition zone diameter of more than 30. 0 mm. The antimicrobial activity of BJ37 fermented broth extracted with different polar solvent was further studied,and the results showed that the bioactive substances were focused on the parts of medium polarity. BJ37 was confirmed to be similar to Streptomyces venezuelae identified by the morphological features and molecular biology analyses. As a potential microbial microbicide,it is worthy of further study.
Fifty-six symbiotic actinomycetes strains were isolated from Harpalus sinicus by plate separation method.Among them strain BJ37 showed the fine antimicrobial activity against Bacillus subtilis and Candida albicans with the inhibition zone diameter of more than 30. 0 mm. The antimicrobial activity of BJ37 fermented broth extracted with different polar solvent was further studied,and the results showed that the bioactive substances were focused on the parts of medium polarity. BJ37 was confirmed to be similar to Streptomyces venezuelae identified by the morphological features and molecular biology analyses. As a potential microbial microbicide,it is worthy of further study.
引文
[1]Hiltunen T,Virta M,Laine AL.Antibiotic resistance in the wild:an eco-evolutionary perspective[J].Philosophical Transactions of the Royal Society of London,2017,372(1712):20160039.
[2]Perry J,Waglechner N,Wright G.The prehistory of antibiotic resistance[J].Cold Spring Harbor Perspectives in Medicine,2016,6(6):a025197.
[3]Fischbach MA,Walsh CT.Antibiotics for emerging pathogens[J].Science,2009,325(5944):1089-1093.
[4]Roemer T,Davies J,Giaever G,et al.Bugs,drugs and chemical genomics[J].Nature Chemical Biology,2012,8(1):46-56.
[5]Chait R,Vetsigian K,Kishony R.What counters antibiotic resistance in nature?[J].Nature Chemical Biology,2011,8(1):2-5.
[6]Li WH,Vederas JC.Drug discovery and natural products:end of era or an endless frontier?[J].Science,2009,325(5937):161-165.
[7]Capon RJ.Microbial biodiscovery:back to the future[J].Current Topics in Medicinal Chemistry,2012,12(14):1471.
[8]Schmidt EW.Trading molecules and tracking targets in symbiotic interactions[J].Nature Chemical Biology,2008,4(8):466-473.
[9]Schoenian I,Spiteller M,Ghaste M,et al.Chemical basis of the synergism and antagonism in microbial communities in the nests of leaf-cutting ants[J].Proceedings of the National Academy of Sciences of the United States of America,2011,108(5):1955-1960.
[10]Oh DC,Poulsen M,Currie CR,et al.Dentigerumycin:a bacterial mediator of an ant-fungus symbiosis[J].Nature Chemical Biology,2009,5(6):391-393.
[11]Zhang Y,Ge H,Zhao W,et al.Unprecedented immunosuppressive polyketides from Daldinia eschscholzii,a mantisassociated fungus[J].Angewandte Chemie International Edition,2008,47(31):5823-5826.
[12]Perianayagam JB,Sharma SK,Pillai KK,et al.Evaluation of antimicrobial activity of ethanol extract and compounds isolated from Trichodesma indicum(Linn.)R.Br.root[J].Journal of Ethnopharmacology,2012,142(1):283-286.
[13]卢贻会,李帅,周端顼,等.白蚁巢拮抗放线菌BYC01代谢产物的分离和鉴定[J].微生物学报,2014,54(7):754-759.
[2]Perry J,Waglechner N,Wright G.The prehistory of antibiotic resistance[J].Cold Spring Harbor Perspectives in Medicine,2016,6(6):a025197.
[3]Fischbach MA,Walsh CT.Antibiotics for emerging pathogens[J].Science,2009,325(5944):1089-1093.
[4]Roemer T,Davies J,Giaever G,et al.Bugs,drugs and chemical genomics[J].Nature Chemical Biology,2012,8(1):46-56.
[5]Chait R,Vetsigian K,Kishony R.What counters antibiotic resistance in nature?[J].Nature Chemical Biology,2011,8(1):2-5.
[6]Li WH,Vederas JC.Drug discovery and natural products:end of era or an endless frontier?[J].Science,2009,325(5937):161-165.
[7]Capon RJ.Microbial biodiscovery:back to the future[J].Current Topics in Medicinal Chemistry,2012,12(14):1471.
[8]Schmidt EW.Trading molecules and tracking targets in symbiotic interactions[J].Nature Chemical Biology,2008,4(8):466-473.
[9]Schoenian I,Spiteller M,Ghaste M,et al.Chemical basis of the synergism and antagonism in microbial communities in the nests of leaf-cutting ants[J].Proceedings of the National Academy of Sciences of the United States of America,2011,108(5):1955-1960.
[10]Oh DC,Poulsen M,Currie CR,et al.Dentigerumycin:a bacterial mediator of an ant-fungus symbiosis[J].Nature Chemical Biology,2009,5(6):391-393.
[11]Zhang Y,Ge H,Zhao W,et al.Unprecedented immunosuppressive polyketides from Daldinia eschscholzii,a mantisassociated fungus[J].Angewandte Chemie International Edition,2008,47(31):5823-5826.
[12]Perianayagam JB,Sharma SK,Pillai KK,et al.Evaluation of antimicrobial activity of ethanol extract and compounds isolated from Trichodesma indicum(Linn.)R.Br.root[J].Journal of Ethnopharmacology,2012,142(1):283-286.
[13]卢贻会,李帅,周端顼,等.白蚁巢拮抗放线菌BYC01代谢产物的分离和鉴定[J].微生物学报,2014,54(7):754-759.