中药一支箭HPLC指纹图谱
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摘要
目的:建立中药一支箭3种基源植物尖头瓶尔小草(Ophioglossum pedunculosum)、狭叶瓶尔小草(O.thermal)和瓶尔小草(O.vulgatum)的HPLC指纹图谱,为科学评价药材质量提供依据。方法:采用HPLC方法,Waters Xbridget色谱柱(4.6 mm×250 mm,5μm),流动相甲醇(A)-0.3%甲酸水(B)梯度洗脱,流速1.0 m L·min-1,柱温40℃,检测波长260 nm,进样量10μL,用聚类分析、相似度分析和主成分分析方法对一支箭指纹图谱进行分析。结果:15批一支箭中药材归为3类,确定14个主要共有峰,在亲缘关系上狭叶瓶尔小草和瓶尔小草较近,离尖头瓶尔小草较远,利用4个对照品瓶尔小草醇,3-O-甲基槲皮素,瓶尔小草醇4'-O-β-D-葡萄糖苷和3-O-甲基槲皮素7-O-β-D-葡萄糖基-4'-O-β-D-葡糖糖苷的相对含量作为化学分类学标签可区分3个种。结论:一支箭HPLC指纹图谱的构建为其基源植物的质量控制和品种鉴定提供了参考。
Objective: To establish the HPLC fingerprints of Ophioglossum pedunculosum,O. vulgatum and O. thermal of O. and provide basis for its quality evaluation. Method: HPLC method was used on Waters Xbridget column( 4. 6 mm × 250 mm,5 μm) with the mobile phase of methanol( A)-0. 3% methanoic acid solution( B) for gradient elution. The flow rate was 1. 0 m L·min- 1and the column temperature was 40 ℃. The detection wavelength was set at 260 nm,and the injection volume was 10 μL. The fingerprints of Ophioglossum were analyzed by cluster analysis,similarity comparative analysis and principal component analysis. Result:Fifteen batches of Ophioglossum were classified into three kinds,and 14 main common peaks were identified in the fingerprints. The genetic relationship of O. vulgatum and O. thermal was close to each other,while far away from O. pedunculosum. Four control substances including ophioglonol,3-O-methylquercetin, ophioglonol4'-O-β-Dglucopyranoside, and 3-O-methylquercetin7-O-β-D-glucopyranosyl-4'-O-β-D-glucopyranoside can be served as chemotaxonomy tags for distinguishing the above three kinds. Conclusion: The establishment of HPLC fingerprint of Ophioglossum provides a reference method for quality control and species identification of its original plants.
Objective: To establish the HPLC fingerprints of Ophioglossum pedunculosum,O. vulgatum and O. thermal of O. and provide basis for its quality evaluation. Method: HPLC method was used on Waters Xbridget column( 4. 6 mm × 250 mm,5 μm) with the mobile phase of methanol( A)-0. 3% methanoic acid solution( B) for gradient elution. The flow rate was 1. 0 m L·min- 1and the column temperature was 40 ℃. The detection wavelength was set at 260 nm,and the injection volume was 10 μL. The fingerprints of Ophioglossum were analyzed by cluster analysis,similarity comparative analysis and principal component analysis. Result:Fifteen batches of Ophioglossum were classified into three kinds,and 14 main common peaks were identified in the fingerprints. The genetic relationship of O. vulgatum and O. thermal was close to each other,while far away from O. pedunculosum. Four control substances including ophioglonol,3-O-methylquercetin, ophioglonol4'-O-β-Dglucopyranoside, and 3-O-methylquercetin7-O-β-D-glucopyranosyl-4'-O-β-D-glucopyranoside can be served as chemotaxonomy tags for distinguishing the above three kinds. Conclusion: The establishment of HPLC fingerprint of Ophioglossum provides a reference method for quality control and species identification of its original plants.
引文
[1]秦仁昌.中国植物志.第2卷[M].北京:北京科学出版社,1959:6-10.
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[5]Lin Y L,Shen C C,Huang Y J,et al.Homoflavonoids from Ophioglossum petiolatum[J].J Nat Prod,2005,68(3):381-384.
[6]Wan C X,Zhang P H,Luo J G,et al.Homoflavonoid glucosides from Ophioglossum pedunculosum and their anti-HBV activity[J].J Nat Prod,2011,74(4):683-689.
[7]王中仁.有梗瓶尔小草叶的多形性和染色体数目[J].植物学报,1986,28(5):472-476.
[8]薛丹丹,张科卫,钱琎.干姜、姜皮和姜炭的HPLC指纹图谱比较研究[J].中国实验方剂学杂志,2015,21(5):57-60.
[9]Wan C X,Luo J G,Gu Y C,et al.Characterisation of homoflavonoids from three Ophioglossum species using liquid chromatography with diode array detection and electrospray ionisation tandem mass spectrometry[J].Phytochem Anal,2013,24(6):541-549.
[2]秦仁昌.中国蕨类植物科属的系统排列和历史来源[J].植物分类学报,1978,16(3):1-19.
[3]赵继祖.中药一枝箭治疗蛇咬伤1例体会[J].甘肃中医,2001,14(4):51.
[4]卢伟,陈晟玮,董心怡.一叶草的性状及显微鉴别[J].福建中医学院学报,2009,19(3):10-13.
[5]Lin Y L,Shen C C,Huang Y J,et al.Homoflavonoids from Ophioglossum petiolatum[J].J Nat Prod,2005,68(3):381-384.
[6]Wan C X,Zhang P H,Luo J G,et al.Homoflavonoid glucosides from Ophioglossum pedunculosum and their anti-HBV activity[J].J Nat Prod,2011,74(4):683-689.
[7]王中仁.有梗瓶尔小草叶的多形性和染色体数目[J].植物学报,1986,28(5):472-476.
[8]薛丹丹,张科卫,钱琎.干姜、姜皮和姜炭的HPLC指纹图谱比较研究[J].中国实验方剂学杂志,2015,21(5):57-60.
[9]Wan C X,Luo J G,Gu Y C,et al.Characterisation of homoflavonoids from three Ophioglossum species using liquid chromatography with diode array detection and electrospray ionisation tandem mass spectrometry[J].Phytochem Anal,2013,24(6):541-549.