抗中间普氏菌卵黄抗体对大鼠妊娠期龈炎疗效的初探
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摘要
目的:制备和纯化抗中间普氏菌特异性卵黄免疫球蛋白(IgY),并评价其对实验性妊娠期大鼠牙龈炎的治疗作用。方法:两步硫酸铵沉淀法获得特异性IgY,将24只雌性SD大鼠随机分为4组,A组(空白组)、B组(阴性组):造模、C组:(阳性组)造模+替硝唑溶液、D组:(实验组)造模+IgY溶液,通过牙龈指数(GI)、菌斑指数(PLI)、体重变化、RT-qPCR,及组织病理学切片观察特异性IgY的治疗作用。结果:通过对大鼠实验性妊娠期牙龈炎的治疗GI、PLI(P<0.001)、体重(P<0.05)均得到改善,龈下菌斑治疗前后中间普氏菌的构成比明显降低(P<0.05)。结论:抗中间普氏菌特异性卵黄抗体可改善大鼠实验性妊娠期的牙龈炎症状况。
Objective: To prepare and purify specific yolk immunoglobulin(IgY) against Prevotella intermedia(P.intermedia, Pi)and to evaluate its therapeutic effect on experimental gingivitis in pregnant rats. Methods: Specific IgY was prepared by two-step ammonium sulfate precipitation method. Twenty-four female SD rats were randomly divided into 4 groups: group A(blank control group), group B(negative group): modeled, group C(positive control group): modeled + tinidazole solution, and group D(experimental group): modeled + specific IgY solution. The therapeutic effect of specific IgY was observed by gingival index(GI), plaque index(PLI), body weight change, RT-qPCR, and histopathological sections. Results: Through the treatment of experimental gestational gingivitis in rats, the GI, PLI(P<0.001) and body weight(P<0.05) were improved, and the composition ratio of P. intermedia was significantly decreased after the treatment of subgingival plaque(P<0.05). Conclusion: Anti-P.intermedia--specific yolk antibody can relief the gingival inflammation in rats during pregnancy
Objective: To prepare and purify specific yolk immunoglobulin(IgY) against Prevotella intermedia(P.intermedia, Pi)and to evaluate its therapeutic effect on experimental gingivitis in pregnant rats. Methods: Specific IgY was prepared by two-step ammonium sulfate precipitation method. Twenty-four female SD rats were randomly divided into 4 groups: group A(blank control group), group B(negative group): modeled, group C(positive control group): modeled + tinidazole solution, and group D(experimental group): modeled + specific IgY solution. The therapeutic effect of specific IgY was observed by gingival index(GI), plaque index(PLI), body weight change, RT-qPCR, and histopathological sections. Results: Through the treatment of experimental gestational gingivitis in rats, the GI, PLI(P<0.001) and body weight(P<0.05) were improved, and the composition ratio of P. intermedia was significantly decreased after the treatment of subgingival plaque(P<0.05). Conclusion: Anti-P.intermedia--specific yolk antibody can relief the gingival inflammation in rats during pregnancy
引文
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[7] 孟明理,徐燕,王晓静,等.抗牙龈素粘附片段Hgp44卵黄抗体对实验性牙周炎的抑制作用[J].口腔医学研究,2016,32(7):699-703.
[8] Hou YY,Zhen YH,Wang D,et al.Protective effect of an egg yolk-derived immunoglobulin (IgY) against Prevotella intermedia-mediated gingivitis[J].J Appl Microbiol,2014,116(4):1020-1027.
[9] ?tefan M,Vojtěch J.Antibiotic therapy in pregnancy [J].Ceska Gynekol,2018,83(1):70-80.
[10] 王晓静,徐燕,孟明理,等.抗中间普氏菌卵黄抗体的制备及其对实验性妊娠期龈炎的预防作用[J].安徽医科大学学报,2017,52(8):1154-1159.
[11] Akbari MR,Ahmadi A,Mirkalantari S.Anti-vibriocholerae IgY antibody inhibits mortality in suckling mice model [J].J Natl Med Assoc,2018,110(1):84-87.
[2] Puertas A,Magan-Fernandez A,Blanc V,et al.Association of periodontitis with preterm birth and low birth weight:a comprehensive review [J].J Matern Fetal Neonatal Med,2018,31(5):597-602.
[3] 陈智慧,吕莉娟,张亮,等.口腔微生物与不良妊娠结局关系的研究进展[J].口腔医学研究,2017,33(8):905-908.
[4] Mallorquí-Fernández N,Manandhar SP,Mallorquí-Fernández G.A new autocatalytic activation mechanism for cysteine proteases revealed by Prevotella intermedia interpain A [J].J Biol Chem,2007,283(5):2871-2882.
[5] Potempa M,Potempa J,Kantyka T,et al.Interpain A,a cysteine proteinase from Prevotella intermedia,inhibits complement by degrading complement factor C3 [J].PLoS Pathog,2009,5(2):e1000316.
[6] Byrne DP,Potempa J,Olczak T,et al.Evidence of mutualism between two periodontal pathogens:Cooperative haem acquisition by the HmuY haemophore of Porphyromonas gingivalis and the cysteine protease interpain A (InpA) of Prevotella intermedia [J].Mol Oral Microbiol,2013,28(3):219-229.
[7] 孟明理,徐燕,王晓静,等.抗牙龈素粘附片段Hgp44卵黄抗体对实验性牙周炎的抑制作用[J].口腔医学研究,2016,32(7):699-703.
[8] Hou YY,Zhen YH,Wang D,et al.Protective effect of an egg yolk-derived immunoglobulin (IgY) against Prevotella intermedia-mediated gingivitis[J].J Appl Microbiol,2014,116(4):1020-1027.
[9] ?tefan M,Vojtěch J.Antibiotic therapy in pregnancy [J].Ceska Gynekol,2018,83(1):70-80.
[10] 王晓静,徐燕,孟明理,等.抗中间普氏菌卵黄抗体的制备及其对实验性妊娠期龈炎的预防作用[J].安徽医科大学学报,2017,52(8):1154-1159.
[11] Akbari MR,Ahmadi A,Mirkalantari S.Anti-vibriocholerae IgY antibody inhibits mortality in suckling mice model [J].J Natl Med Assoc,2018,110(1):84-87.