陕南紫阳富硒茶有机硒提取方法的研究
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摘要
研究建立一种从安康紫阳富硒茶中提取有机硒方法,利用相似相溶原理,选取乙醇、乙酸乙酯、环己烷对茶叶中的有机硒进行提取,得出在pH 7时,用乙醇回流法提取有机硒效果最好;通过L9(34)正交试验优化提取工艺,确定最佳提取条件为乙醇体积分数60%、回流温度70℃、固液比1∶19(g/mL)、加热回流时间2 h。
A method for extracting organic selenium from Ziyang selenium-rich tea in Ankang was established. Using the principle of similar solubility, ethanol, ethyl acetate and cyclohexane were selected to extract organic selenium from tea. It was concluded that when pH was 7, ethanol reflux method was the best method for extracting organic selenium. The extraction process was optimized by L9(34) orthogonal test, and the optimum extraction conditions were determined. The results showed that with 60% ethanol, 70 degree temperature, solid-liquid ratio of 1∶19(g/mL), heating reflux extraction time of 2 hours,organic selenium extraction in tea is the best.
A method for extracting organic selenium from Ziyang selenium-rich tea in Ankang was established. Using the principle of similar solubility, ethanol, ethyl acetate and cyclohexane were selected to extract organic selenium from tea. It was concluded that when pH was 7, ethanol reflux method was the best method for extracting organic selenium. The extraction process was optimized by L9(34) orthogonal test, and the optimum extraction conditions were determined. The results showed that with 60% ethanol, 70 degree temperature, solid-liquid ratio of 1∶19(g/mL), heating reflux extraction time of 2 hours,organic selenium extraction in tea is the best.
引文
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[3] MIGUEL N A,CARMEN C V. Selenium in food and the human body:a review[J].Sci Total Environ,2008,4009(1):115-141.
[4]贾奎寿,叶素芳.微量元素硒与人体健康的研究[J].广东微量元素科学,2002,9(1):4.
[5]徐芳,邱德仁,胡克季,等.硒的化学与生物化学形态分析综述[J].光谱学与光谱分析,2002,22(2):331-340.
[6] RAYMA M P. The use of high-selenium yeast to raise selenium status:how does it measure up[J].Brit J Nutr,2004,92(4):557-573.
[7] MCKENZIE M J,HUNTER D A,PATHIRANA R,et al. Accumulation of an organic anticancer selenium compound in atransgenic Solanaceous species shows wider applicability of the selenocysteine methyltransferase transgene from selenium hyperaccumulators[J].Transgenic Res,2009,18(3):407-424.
[8] GB 5009. 93-2017,食品安全国家标准食品中硒的测定[S].
[9]杨潇,芮光伟,许海东.微波消解-石墨炉原子吸收光谱法检测大米中硒元素的含量[J].食品与发酵工业,2010,36(1):138-141.
[10]肖辉,吴向阳,仰榴青,等.氢化物-原子荧光法测定姬松茸子实体中硒含量[J].食品科学,2010,31(10):247-249.
[11]郝素娥,滕冰.硒酵母中有机硒及硒代氨基酸含量的测定方法[J].分析测试学报,1999,18(3):72-74.
[12]高建忠,秦顺义,黄克和.氢化物发生-原子荧光光谱法测定富硒酵母中的有机硒和无机硒[J].分析科学学报,2006,22(2):157-160.
[13]崔海容,陈建华,谷家越,等.顺序注射HG-AFS法测定富硒农产品中无机硒和有机硒[J].分析科学学报,2005,21(5):545-548.
[14]金松子,蔡振华,蒋庆峰.分光光度法测定富硒蛋粉中的硒含量[J].现代食品与药品杂志,2007,17(1):37-39.
[15]郝莉鹏,詹铭,郑嵘,等.上海市售鸡蛋硒含量测定[J].实用预防医学,2003,10(1):99-100.
[16]孙新涛,马往校,张双隽.食品中有机硒的测定方法的研究[J].陕西农业科学,2014,60(2):35-37.
[17]张延,张胜义,高亮.环境水样中硒、砷的联合消解和原子荧光法测定[J].安徽大学学报(自然科学版),2007,31(1):74-77.