辛伐他汀对大鼠矽肺的治疗作用
|
摘要
目的观察辛伐他汀对矽肺大鼠模型的影响,探讨其治疗矽肺的作用机制。方法 40只SD大鼠随机分为4组:正常对照组(蒸馏水10mL/kg)、矽肺模型组、辛伐他汀小剂量组(10mg/kg)、辛伐他汀大剂量组(20mg/kg),每组10只。采用气管内注入SiO2造成大鼠矽肺模型,连续给药28d后,观察辛伐他汀对矽肺大鼠肺系数、全肺干重/体重比值、肺组织形态学改变,检测SOD活力、MDA、NO含量的变化。结果辛伐他汀可显著降低矽肺大鼠体重、肺系数及全肺干重/体重比值,明显改善矽肺大鼠肺泡炎细胞浸润、充血水肿及肺间质纤维化程度,降低矽结节分级计分,明显提高矽肺大鼠肺组织匀浆总SOD活力,降低总MDA、NO含量。结论辛伐他汀对大鼠矽肺有治疗作用,其作用机制与降低自由基水平有关。
Objective To observe the effects of simvastatin on silicosis in rats and the possible mechanism of the therapeutic effects.Methods Silicosis of rats was induced by intratracheal injection of silicon dioxide.Forty rats were randomly divided into 4 groups: normal control group(distilled water,10 mL·kg-1),model group and simvastatin groups(10 mg/kg and 20 mg/kg),with 10 rats in each group.After the rats were treated for 28 days with corresponding intragastric administration,their lung index,ratio of dry weight of whole lung to body weight,lung histomorphology,SOD activities as well as MDA and NO contents were detected to evaluate the effects of simvastatin on silicosis in rats.Results Simvastatin reduced the body weight,lung index,and ratio of dry weight of whole lung to body weight.Simvastatin ameliorated obviously inflammation cell infiltration,congestion and dropsy of alveoli pulmonis,pulmonary interstitial fibrosis,and decreased the grade scores of silicotic nodule.It increased markedly SOD activities,but decreased MDA and NO contents in lung tissues of silicosis rats.Conclusion The therapeutic effect of simvastatin on silicosis in rats is related to the decreased level of free radicals.
Objective To observe the effects of simvastatin on silicosis in rats and the possible mechanism of the therapeutic effects.Methods Silicosis of rats was induced by intratracheal injection of silicon dioxide.Forty rats were randomly divided into 4 groups: normal control group(distilled water,10 mL·kg-1),model group and simvastatin groups(10 mg/kg and 20 mg/kg),with 10 rats in each group.After the rats were treated for 28 days with corresponding intragastric administration,their lung index,ratio of dry weight of whole lung to body weight,lung histomorphology,SOD activities as well as MDA and NO contents were detected to evaluate the effects of simvastatin on silicosis in rats.Results Simvastatin reduced the body weight,lung index,and ratio of dry weight of whole lung to body weight.Simvastatin ameliorated obviously inflammation cell infiltration,congestion and dropsy of alveoli pulmonis,pulmonary interstitial fibrosis,and decreased the grade scores of silicotic nodule.It increased markedly SOD activities,but decreased MDA and NO contents in lung tissues of silicosis rats.Conclusion The therapeutic effect of simvastatin on silicosis in rats is related to the decreased level of free radicals.
引文
[1]YAO SQ,ROJANASAKUL LW,CHEN ZY,et al.Fas/FasLpathway-mediated alveolar macrophage apoptosis involved in hu-man silicosis[J].Apoptosis,2011,16(12):1195-1204.
[2]周效宝,王瑞.细胞因子在矽肺发生发展中作用研究进展[J].中国公共卫生,2011,27(12):1630-1632.
[3]CHIDA M,FUKUDA H,ARAKI O,et al.Lung transplanta-tion for aspiration-induced silicosis of the lung[J].Gen ThoracCardiovasc Surg,2010,58(3):141-143.
[4]KIM JW,RHEE CK,KIM TJ,et al.Effect of pravastatin onbleomycin-induced acute lung injury and pulmonary fibrosis[J].Clin Exp Pharmacol Physiol,2010,37(11):1055-1063.
[5]李倩,闫静波,张丽娟,等.抗纤维化短肽对大鼠矽肺纤维化MCP-1、ED-1表达的影响[J].卫生研究,2008,37(6):666-670.
[6]王素华,杜茂林,张翼翔,等.100例矽肺患者血清中NO、LPO、SOD的观察[J].中国工业医学杂志,2009,22(2):122-123.
[7]ZHANG JW,LV GC,YAO JM,et al.Assessment of serum an-tioxidant status in patients with silicosis[J].J Int Med Res.2010,38(3):884-889.
[8]张雪涛,倪为民,缪荣明,等.矽肺患者氧化应激指标及外周血单核细胞NF-κB水平的变化[J].中华劳动卫生职业病杂志,2011,29(4):251-254.
[9]SAUNI R,OKSA P,LEHTIMAKI L,et al.Increased alveolarnitric oxide and systemic inflammation markers in silica-exposedworkers[J].Occup Environ Med,2012,69(4):256-260.
[2]周效宝,王瑞.细胞因子在矽肺发生发展中作用研究进展[J].中国公共卫生,2011,27(12):1630-1632.
[3]CHIDA M,FUKUDA H,ARAKI O,et al.Lung transplanta-tion for aspiration-induced silicosis of the lung[J].Gen ThoracCardiovasc Surg,2010,58(3):141-143.
[4]KIM JW,RHEE CK,KIM TJ,et al.Effect of pravastatin onbleomycin-induced acute lung injury and pulmonary fibrosis[J].Clin Exp Pharmacol Physiol,2010,37(11):1055-1063.
[5]李倩,闫静波,张丽娟,等.抗纤维化短肽对大鼠矽肺纤维化MCP-1、ED-1表达的影响[J].卫生研究,2008,37(6):666-670.
[6]王素华,杜茂林,张翼翔,等.100例矽肺患者血清中NO、LPO、SOD的观察[J].中国工业医学杂志,2009,22(2):122-123.
[7]ZHANG JW,LV GC,YAO JM,et al.Assessment of serum an-tioxidant status in patients with silicosis[J].J Int Med Res.2010,38(3):884-889.
[8]张雪涛,倪为民,缪荣明,等.矽肺患者氧化应激指标及外周血单核细胞NF-κB水平的变化[J].中华劳动卫生职业病杂志,2011,29(4):251-254.
[9]SAUNI R,OKSA P,LEHTIMAKI L,et al.Increased alveolarnitric oxide and systemic inflammation markers in silica-exposedworkers[J].Occup Environ Med,2012,69(4):256-260.