基质金属蛋白酶-9及抑癌基因PTEN在子宫内膜异位症中的表达及意义
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摘要
目的探讨基质金属蛋白酶-9(MMP-9)及人第10号染色体缺失的磷酸酶及张力蛋白同源基因(PTEN)在子宫内膜异位症(Ems)发生、发展中的作用。方法应用免疫组化SP法检测Ems异位内膜62例(其中子宫来源Ems 40例,卵巢来源Ems 22例)、Ems在位内膜60例以及正常子宫内膜41例中MMP-9、PTEN的表达情况。结果异位组MMP-9的阳性表达率较对照组(正常子宫内膜)明显升高,而PTEN的阳性表达率较对照组显著降低,均有统计学差异(均P<0.05)。异位组中MMP-9的表达与PTEN的表达呈负相关(r=-0.472,P<0.05)。结论 Ems患者中异位内膜具有更强的侵袭性,可能与异位内膜MMP-9高表达及PTEN的低表达相关。
Objective To investigate the expression of matrix metalloproteinase-9(MMP-9) and phosphatase and tensin homolog deleted on chromosome ten(PTEN) protein in endometriosis(EM) and its clinical significance.Methods Immunohistochemical SP method was employed to detect the expression of MMP-9 and PTEN in 62 samples of ectopic endometrium from EM patients(including 40 adenomyosis and 22 ovarian endometriosis),60 samples of eutopic endometrium from EM patients and41 samples of normal endomedium(controls).Results The expression levels of MMP-9 in the ectopic endometrium were higher and the expression levels of PTEN were lower than those in controls(both P<0.05).The expression of MMP-9 in ectopic endometrium was negatively correlated with the expressions of PTEN(r=-0.472,P<0.05).Conclusion Ectopic endometrium has increased expression of MMP-9 and decreased expression of PTEN,which may be associated with its high invasiveness.
Objective To investigate the expression of matrix metalloproteinase-9(MMP-9) and phosphatase and tensin homolog deleted on chromosome ten(PTEN) protein in endometriosis(EM) and its clinical significance.Methods Immunohistochemical SP method was employed to detect the expression of MMP-9 and PTEN in 62 samples of ectopic endometrium from EM patients(including 40 adenomyosis and 22 ovarian endometriosis),60 samples of eutopic endometrium from EM patients and41 samples of normal endomedium(controls).Results The expression levels of MMP-9 in the ectopic endometrium were higher and the expression levels of PTEN were lower than those in controls(both P<0.05).The expression of MMP-9 in ectopic endometrium was negatively correlated with the expressions of PTEN(r=-0.472,P<0.05).Conclusion Ectopic endometrium has increased expression of MMP-9 and decreased expression of PTEN,which may be associated with its high invasiveness.
引文
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[4]李艳,陈琼霞,沈延春,等抑癌基因PTEN在子宫内膜异位症中的表达及意义[J].中外医疗,2009,28(25):151-152,155
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[8]蒋苹,于璐,孙洁,等.PTEN、Survivin蛋白在卵巢子宫内膜异位囊肿表达的相关性及临床意义[J].吉林医学,2014,35(16):3429-3430.
[9]Zhang H.Zhao X.Liu S.et al.17betaE2 promotes cell proliferation in endometriosis by decreasing PTEN via NFkappaB-dependent pathway[J].Mol Cell Endocrinol,2010,317(1-2):31-43.
[10]Moon S K.Kim H M.Kim C H.PTEN induces G1 cell cycle arrest and inhibits MMP-9 expression via the regulation of NF-Kappa B and AP-1 in vascular smooth muscle cells[J].Arch Biochem Biophys,2004,421(2):267-276.
[11]覃月秋,黄赞松,何守搞,等.胃癌组织PTEN基因的表达及其与MMP-9的相关性[J].中国老年学杂志,2006,30(6):1646-1648.
[12]张清华,李少林,彭志平,等PTEN与MMP-9在子宫内膜癌中的表达及临床意义[J].肿瘤,2006,26(5):476-479.
[2]Wang C X,Zhou H Y,You X N,et al.An investigation of the potential malignant characteristics of endometriosis[J].Chinese Journal of Clinical Oncology,2005,2(6):861-865.
[3]王丽,李东爽,郭雪霏基质金属蛋白酶及其抑制物在正常和异位子宫内膜中的表达[J].浙江临床医学,2011,13(4):364-365.
[4]李艳,陈琼霞,沈延春,等抑癌基因PTEN在子宫内膜异位症中的表达及意义[J].中外医疗,2009,28(25):151-152,155
[5]Govatati S,Kodati V L.Deenadayal M,et al.Mutations in the PTEN tumor gene and risk of endometriosis:a case-control study[J].Hum Reprod,2014,29(2):324-336.
[6]Song M S.Saimena L.Pandolfi P P.The functions and regulation of the PTEN tumour suppressor[J].Nat Rev Mol Cell Biol,2012.13(5):283-296.
[7]何宏月,朱颖军,王芳,等.子宫内膜异位症患者病变组织中miRNA-21、PTEN蛋白的表达变化及意义[J].山东医药,2014,54(27):67-69.
[8]蒋苹,于璐,孙洁,等.PTEN、Survivin蛋白在卵巢子宫内膜异位囊肿表达的相关性及临床意义[J].吉林医学,2014,35(16):3429-3430.
[9]Zhang H.Zhao X.Liu S.et al.17betaE2 promotes cell proliferation in endometriosis by decreasing PTEN via NFkappaB-dependent pathway[J].Mol Cell Endocrinol,2010,317(1-2):31-43.
[10]Moon S K.Kim H M.Kim C H.PTEN induces G1 cell cycle arrest and inhibits MMP-9 expression via the regulation of NF-Kappa B and AP-1 in vascular smooth muscle cells[J].Arch Biochem Biophys,2004,421(2):267-276.
[11]覃月秋,黄赞松,何守搞,等.胃癌组织PTEN基因的表达及其与MMP-9的相关性[J].中国老年学杂志,2006,30(6):1646-1648.
[12]张清华,李少林,彭志平,等PTEN与MMP-9在子宫内膜癌中的表达及临床意义[J].肿瘤,2006,26(5):476-479.