不同光照周期对鸡睾酮合成的影响
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摘要
为研究不同光照周期对公鸡睾酮合成的影响,试验选择120只20周龄体重相近的AA(爱拔益加)公鸡,随机分配到3个不同光照处理组,SD、CTR、LD组分别采用8L:16D、12.5L:11.5D、16L:8D光照周期,4周后采集血清和睾丸;用ELISA技术检测睾酮含量,用实时荧光定量PCR和Westernblot技术检测睾酮合成的3个关键酶(StAR、Cyp11a1、3β-HSD)表达量,HE染色观察睾丸中曲细精管横截面积。结果表明:睾酮含量随光照周期延长极显著增加(P<0.01),并伴随睾酮分泌关键酶StAR、Cyp11a1、和3β-HSD表达量极显著升高(P<0.01),曲细精管横截面积极显著增大(P<0.01)。综上,不同光照周期可通过影响睾酮合成3个关键酶表达而影响睾酮合成,随着光照时间延长,睾酮合成增加,睾丸生精功能增强。
Effects of different light durations on testosterone synthesis in roosters. 120 AA(Arbor Acres) roosters with similar body weights of 20 weeks old were randomly assigned to three different light treatment groups(8 h, 11.5 h, 16 h), and then subjected to different light treatment for 28 days. The ELISA kit was used to detect the testosterone content and the testis was used after carotid artery bleeding which leads to death. The qRT-PCR and western blot were used to detect the changes in testosterone secretion key enzymes(StAR, Cyp11 a1, 3β-HSD). HE staining was used to observe the area of seminiferous tubules in the testes. Testosterone synthesis increased significantly with the prolongation of light duration(P <0.01),accompanied by increased expression of key enzymes in testosterone secretion(P<0.01).Conclusion: Different light treatments affected the testosterone synthesis in roosters, and long-time light treatment was also beneficial to testosterone synthesis and esticular spermatogenesis.
Effects of different light durations on testosterone synthesis in roosters. 120 AA(Arbor Acres) roosters with similar body weights of 20 weeks old were randomly assigned to three different light treatment groups(8 h, 11.5 h, 16 h), and then subjected to different light treatment for 28 days. The ELISA kit was used to detect the testosterone content and the testis was used after carotid artery bleeding which leads to death. The qRT-PCR and western blot were used to detect the changes in testosterone secretion key enzymes(StAR, Cyp11 a1, 3β-HSD). HE staining was used to observe the area of seminiferous tubules in the testes. Testosterone synthesis increased significantly with the prolongation of light duration(P <0.01),accompanied by increased expression of key enzymes in testosterone secretion(P<0.01).Conclusion: Different light treatments affected the testosterone synthesis in roosters, and long-time light treatment was also beneficial to testosterone synthesis and esticular spermatogenesis.
引文
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[2]Chang S C,Li n M J,Fan Y K,et al.Effects of lighti ng intensity on growth and reproductive performance of breeder geese[J].J Appl Poult Res,2016,25(3):9.
[3]关羽.环境对家禽饲养的影响及其控制[J].现代畜牧科技,2017(11):38.
[4]孙研研,陈继兰.种公禽繁殖系统对光要素的应答机制研究进展[J].中国畜牧兽医,2017,44(9):2692-2698.
[5]张振汉,张志旺,于洪川.缺乏光照对雄性小鼠性腺发育的影响[J].甘肃畜牧兽医,2007,37(4):9-12.
[6]李琴,马鸿鹏,刘安芳,等.光照影响鹅繁殖性能和激素水平的研究进展[J].中国畜牧杂志,2015,51(15):88-92.
[7]Smith G T,Brenowitz E A,Wingf ield J C.Roles of photoperiod and testosterone in seasonal plasticity of the avian song control system[J].J Neurobiol,2015,32(4):426-442.
[8]金宝栋.光照处理对东北籽鹅血清生殖激素水平和产蛋量的影响[D].大庆:黑龙江八一农垦大学,2015.
[9]王莹,崔一吉吉,武超,等.爱拔益加肉鸡父母代后备期的生长性能分析[J].畜牧与饲料科学,2016,37(10):25-27.
[10]Szwed A,Kosinska M,Manning J T.Digit ratio(2D:4D)and month of birth:A link to the solstitial-melatonin-testosterone effect[J].Early Hum Dev,2017,104:23.
[11]Hébert M,Martin S K,Lee C,et al.The effects of prior light history on the suppression of melatonin by light in humans[J].J Pineal Res,2010,33(4):198-203.
[12]谢亮,罗婷,胡妍钰,等.光照因素对褪黑素分泌及性早熟形成影响的研究进展[J].世界最新医学信息文摘,2016,16(55):26-27.
[13]Guan S,Xie L,Ma T,et al.Effects of melatonin on early pregnancy in mouse:involving the regulation of StAR,Cyp11a1,and Ihh expression[J].Int J Mol Sci,2017,18(8):1637.
[14]幺学博.种公鸡繁殖性能的影响因素[J].新农业,2017(17):38-40.