扎龙湿地两种表型芦苇(Phragmites australis)的形态学特性分析
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摘要
为了比较芦苇不同生长发育性状,本研究利用显微及扫描电子显微镜比较普通芦苇和铁秆芦苇的形态结构特征。二者株高、叶宽、叶长和茎粗指标差异均不显著(p>0.05);植株鲜重、干重、叶表皮气孔数、气孔长径和气孔宽径指标二者差异极显著(p<0.01),茎厚壁组织厚度和维管束直径差异极显著(p<0.01),薄壁组织厚度差异不显著(p>0.05)。种子形态指标差异均极显著(p<0.01),铁秆芦苇种子的长、宽更长及千粒重更大。结果表明铁秆芦苇是芦苇的一种新的生态型,为优异的芦苇种质资源。本研究为筛选优质的芦苇种质提供数据参考。
In order to compare the different growth and development characters of Phragmites australis. The morphological features of common reed and Tiegan reed were compared with microscope and scanning electron microscope. There were no significant difference in plant height, leaf width, leaf length and stem diameter index(p>0.05). Plant fresh weight, dry weight, stomatal number, stomata length, stomata width index of leaf epidermis were extremely significant difference(p<0.01), thickness of stem sclerenchyma and diameter of vascular bundle were significantly different(p<0.01), thickness of parenchyma was not significant different(p>0.05). The differences in seed morphological indexes were extremely significant(p<0.01). The length, width and thousand grain weight of Tiegan reeds were bigger. The results showed that Tiegan reed is a new ecological type of reed, which is an excellent germplasm resource of reed. This study provides data reference for screening high quality reed germplasm.
In order to compare the different growth and development characters of Phragmites australis. The morphological features of common reed and Tiegan reed were compared with microscope and scanning electron microscope. There were no significant difference in plant height, leaf width, leaf length and stem diameter index(p>0.05). Plant fresh weight, dry weight, stomatal number, stomata length, stomata width index of leaf epidermis were extremely significant difference(p<0.01), thickness of stem sclerenchyma and diameter of vascular bundle were significantly different(p<0.01), thickness of parenchyma was not significant different(p>0.05). The differences in seed morphological indexes were extremely significant(p<0.01). The length, width and thousand grain weight of Tiegan reeds were bigger. The results showed that Tiegan reed is a new ecological type of reed, which is an excellent germplasm resource of reed. This study provides data reference for screening high quality reed germplasm.
引文
Lin W.F.,Chen L.J.,and Zhu X.Y.,2007,An analysis of genetic diversity of different ecotypes of reed(Phragmites communis Trin.)by molecular marker techniques,Zhiwu Shengli Yu Fenzi Shengwu Xuebao(Journal of Plant Physiology and Molecular Biology),33(1):77-84(林文芳,陈林娇,朱学艺,2007,用分子标记技术分析不同生态型芦苇的遗传多样性,植物生理与分子生物学报,33(1):77-84)
Wang J.G.,Chen G.C.,and Zhang C.L.,2002,The effects of water stress on soluble protein content,the activity of SOD,POD and CAT of two ecotypes of reeds(Phragmites communis),Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),22(3):561-565(王俊刚,陈国仓,张承烈,2002,水分胁迫对2种生态型芦苇(Phragmites communis)的可溶性蛋白含量、SOD、POD、CAT活性的影响,西北植物学报,22(3):561-565)
Wang M.,Wang Y.B.,and Chen Z.H.,2010,Germplasm resource of phragmites adans and its application in constructed wetlands,Yingyong Yu Huanjing Shengwu Xuebao(Chinese Journal of Applied and Environmental Biology),16(4):590-595(王萌,王玉彬,陈章和,2010,芦苇的种质资源及在人工湿地中的应用,应用与环境生物学报,16(4):590-595)
Wang Q.H.,Li C.,Chen C.,Chen J.,Ma X.J.,and Que X.E.,2017,Physiological responses of Phragmites australis to atrazine exposure and their relevance for tolerance,Nongye Huanjing Kexue Xuebao(Journal of Agro-Environment Science),36(10):1968-1977(王庆海,李翠,陈超,陈洁,马雪菊,却晓娥,2017,芦苇对阿特拉津胁迫的生理响应及其与耐受性的关系,农业环境科学学报,36(10):1968-1977)
Wu C.,Lin Q.Q.,Qin D.H.,Ding X.Y.,and Guo F.Q.,2016,Genetic diversity of cut flower lilies(Lilium)germplasm resources by morphological traits,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),14(5):1300-1308(吴超,林巧奇,秦德辉,丁晓瑜,郭方其,2016,切花百合种质资源表型性状遗传多样性分析,分子植物育种,14(5):1300-1308)
Zhuang Y.,Sun Y.X.,Wang Z.S.,Yang L.L.,Deng Z.F.,Yao Z.G.,and An S.Q.,2010,Research advances in ecotypes of Phragmites australis,Shengtai Xuebao(Acta Ecologica Sinica),30(8):2173-2181(庄瑶,孙一香,王中生,杨琳璐,邓自发,姚志刚,安树青,2010,芦苇生态型研究进展,生态学报,30(8):2173-2181)
Zhang Y.,Yang Y.,Tian L.B.,and Shang S.,2016,Evaluation and genetic diversity of bitter gourd varieties based on phenotypic traits,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),14(1):239-250(张燕,杨衍,田丽波,商桑,2016,基于表型性状的苦瓜种质资源评价和遗传多样性的分析,分子植物育种,14(1):239-250)
Wang J.G.,Chen G.C.,and Zhang C.L.,2002,The effects of water stress on soluble protein content,the activity of SOD,POD and CAT of two ecotypes of reeds(Phragmites communis),Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),22(3):561-565(王俊刚,陈国仓,张承烈,2002,水分胁迫对2种生态型芦苇(Phragmites communis)的可溶性蛋白含量、SOD、POD、CAT活性的影响,西北植物学报,22(3):561-565)
Wang M.,Wang Y.B.,and Chen Z.H.,2010,Germplasm resource of phragmites adans and its application in constructed wetlands,Yingyong Yu Huanjing Shengwu Xuebao(Chinese Journal of Applied and Environmental Biology),16(4):590-595(王萌,王玉彬,陈章和,2010,芦苇的种质资源及在人工湿地中的应用,应用与环境生物学报,16(4):590-595)
Wang Q.H.,Li C.,Chen C.,Chen J.,Ma X.J.,and Que X.E.,2017,Physiological responses of Phragmites australis to atrazine exposure and their relevance for tolerance,Nongye Huanjing Kexue Xuebao(Journal of Agro-Environment Science),36(10):1968-1977(王庆海,李翠,陈超,陈洁,马雪菊,却晓娥,2017,芦苇对阿特拉津胁迫的生理响应及其与耐受性的关系,农业环境科学学报,36(10):1968-1977)
Wu C.,Lin Q.Q.,Qin D.H.,Ding X.Y.,and Guo F.Q.,2016,Genetic diversity of cut flower lilies(Lilium)germplasm resources by morphological traits,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),14(5):1300-1308(吴超,林巧奇,秦德辉,丁晓瑜,郭方其,2016,切花百合种质资源表型性状遗传多样性分析,分子植物育种,14(5):1300-1308)
Zhuang Y.,Sun Y.X.,Wang Z.S.,Yang L.L.,Deng Z.F.,Yao Z.G.,and An S.Q.,2010,Research advances in ecotypes of Phragmites australis,Shengtai Xuebao(Acta Ecologica Sinica),30(8):2173-2181(庄瑶,孙一香,王中生,杨琳璐,邓自发,姚志刚,安树青,2010,芦苇生态型研究进展,生态学报,30(8):2173-2181)
Zhang Y.,Yang Y.,Tian L.B.,and Shang S.,2016,Evaluation and genetic diversity of bitter gourd varieties based on phenotypic traits,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),14(1):239-250(张燕,杨衍,田丽波,商桑,2016,基于表型性状的苦瓜种质资源评价和遗传多样性的分析,分子植物育种,14(1):239-250)