几种不同的化学预处理方法对甜高粱秆酶解糖化效果的影响
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摘要
以甜高粱秸秆为原料,选取不同浓度梯度的盐酸、亚硫酸、甲酸、氢氧化钠、双氧水等分别进行预处理,对双酶酶解的糖化效果进行了研究。研究结果表明,用1%的稀盐酸在121℃高温条件下预处理60 min后,加入纤维素酶2.5g/100g和木聚糖酶2g/100g,MgCl_2溶液2.5 nmol/L,吐温-80为1mL/100g,固液比为110(W/V),pH5.0,50℃酶解糖化48 h,在此条件下还原糖得率最高,其值为29.1%。
With the sweet sorghum straw as the raw material, choosing different concentrations of hydrochloric acid, of sulfurous acid, of formic acid, of sodium hydroxide, and of hydrogen peroxide as pretreatments respectively,the researchers studied the double enzyme effect of hydrolysis. The results show that the reducing sugar yield peaks at 29.1% when the researchers use 1% dilute hydrochloric acid, as pretreatment, at 121 ℃ and join cellulase 2.5 g/100 g, xylanase 2 g/100 g, MgCl2 solution 2.5 nmol/L, and polysorbate 801 mL/100 g after 60 minutes, with the solid-liquid ratio at 1 GA6 FA10(W/V), pH at 5.0, and enzyme saccharification at 50 ℃for 48 h.
With the sweet sorghum straw as the raw material, choosing different concentrations of hydrochloric acid, of sulfurous acid, of formic acid, of sodium hydroxide, and of hydrogen peroxide as pretreatments respectively,the researchers studied the double enzyme effect of hydrolysis. The results show that the reducing sugar yield peaks at 29.1% when the researchers use 1% dilute hydrochloric acid, as pretreatment, at 121 ℃ and join cellulase 2.5 g/100 g, xylanase 2 g/100 g, MgCl2 solution 2.5 nmol/L, and polysorbate 801 mL/100 g after 60 minutes, with the solid-liquid ratio at 1 GA6 FA10(W/V), pH at 5.0, and enzyme saccharification at 50 ℃for 48 h.
引文
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[13]CAO W X,SUN C,LIU R H,et al.Comparison of the effects of five pretreatment methods on enhancing the enzymatic digestibility and ethanol production from sweet sorghum bagasse[J].Bioresour Technol,2012(111s):215-221.
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[16]AGBOR V B,CICEK N,SPARLING R,et al.Biomass pretreatment:fundamentals toward application[J].Biotechnology Adv,2011(29):675-685.
[17]URBANEJA G,FERRER J,PAEA G,et al.Acid hydrolysis and carbohydrates characterization of coffee pulp [J].Renewable Energy,1996,9(1):1041-1044.
[18]SUN R C.Comparative study of lignins isolated by alkali and ultrasound-assisted alkali extractions from wheat straw [J].Ultrasonics Sonochemistry,2002,9(2):85-93.
[2]班靖洋,张栩,谭天伟.以甜高粱渣为原料发酵生产乙醇[J].北京化工大学学报,2007,34(6):637-639.
[3]边文祥,田瑞华,段开红,等.甜高粱秸秆酒糟复合饲料的初步研制[J].畜牧与饲料科学,2010(1):37-38.
[4]刘旭,马春红,及增发,等.利用甜高粱秸秆厌氧发酵制氢的研究[J].云南农业大学学报(自然科学版),2013(1):140-144.
[5]杜晓雪,王春光,郭文斌,等.甜高粱秸秆综合利用及收储运机械化现状分析[J].农机化研究,2018(7):263-268.
[6]李冀新,武冬梅,王吉亮,等.甜高粱制乙醇的主要问题及解决方法[J].新疆农垦科技,2009(4):68-70.
[7]LIN Y,TANAKA S.Ethanol fermentation from biomass resources:Current state and prospects[J].Applied Microbiology and Biotechnology,2016,69( 206):627-642.
[8]OSTOVAREH S,KARIMI K,ZAMANI A.Efficient conversion of sweet sorghum stalks to biogas and ethanol using organosolv pretreatment[J].Industrial Crops & Products,2014,66:170-177.
[9]梅晓岩,刘荣厚.中国甜高粱茎秆制取乙醇的研究进展[J].中国农学通报,2010(5):341-345.
[10]Sun X Z,Minowa T,Yamaguchi K,et al.Evaluation of energy consumption and greenhouse gas emissions from poly(phenyllactic acid) production using sweet sorghum[J].Journal of Cleaner Production,2014,87:208-215.
[11]CHRISTENSEN C H,RASS-HANSEN J,MARSDEN C C,et al.The renewable chemicals industry[J].ChemSusChem,2008,1(4) :283-289.
[12]LI X,MUPONDWA E,PANIGRAHI S,et al.A review of agricultural crop residue supply in Canada for cellulosic ethanol production[J].Renewable& Sustainable Energy Reviews,2012,16:2954-2965.
[13]CAO W X,SUN C,LIU R H,et al.Comparison of the effects of five pretreatment methods on enhancing the enzymatic digestibility and ethanol production from sweet sorghum bagasse[J].Bioresour Technol,2012(111s):215-221.
[14]ANTIZAR-LADISLAO B,Turrion-Gomez J.Second-generation biofuels and local bioenergy systems[J].Biofuels Bioprod Bioref,2008(2):455-469.
[15]AZAM M M,WARIS A,NAHAR N M.Prospects and potential of fatty acid methyl esters of some non-traditional seed oils for use as biodiesel in India[J].Biomass and Bioenergy,2005(29):293-302.
[16]AGBOR V B,CICEK N,SPARLING R,et al.Biomass pretreatment:fundamentals toward application[J].Biotechnology Adv,2011(29):675-685.
[17]URBANEJA G,FERRER J,PAEA G,et al.Acid hydrolysis and carbohydrates characterization of coffee pulp [J].Renewable Energy,1996,9(1):1041-1044.
[18]SUN R C.Comparative study of lignins isolated by alkali and ultrasound-assisted alkali extractions from wheat straw [J].Ultrasonics Sonochemistry,2002,9(2):85-93.