利用超声波处理玉米种子芽对农杆菌转化的影响
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摘要
为提高玉米种子芽生长点的转化效率,研究利用超声波处理玉米种子芽时间对农杆菌转化效果的影响。结果表明,利用超声波频率45 KHZ、超声波功率50 W的超声波仪处理10~15 min,有利于提高农杆菌对玉米的转化效果。表明利用超声波处理玉米芽是一种好的受体处理方法。
In this study, we used ultrasonic to treat the growing point of maize seed bud for different times, aim-ing to improve its transformation efficiency. The efficiency of maize growing point transformation mediated by Agro-bacterium was significantly improved when it was treated by ultrasonic with frequency of 45 kHz and power of 50 Wfor 10-15 min. Thus, it is a good way for improving transformation efficiency mediated by Agrobacterium to treat re-ceptor with ultrasonic.
In this study, we used ultrasonic to treat the growing point of maize seed bud for different times, aim-ing to improve its transformation efficiency. The efficiency of maize growing point transformation mediated by Agro-bacterium was significantly improved when it was treated by ultrasonic with frequency of 45 kHz and power of 50 Wfor 10-15 min. Thus, it is a good way for improving transformation efficiency mediated by Agrobacterium to treat re-ceptor with ultrasonic.
引文
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[6]南芝润,惠国强,罗绮,等.超声波辅助花粉介导法获得转BtCrylAc基因玉米的研究[J].黑龙江农业科学,2015(9):22-25.Nan Z R, Hui G Q, Luo Q, et al. Research on BtCrylAc transgenicmaize lines using ultrasonic-assisted pollen mediated plant transfor-mation method[J]. Heilongjiang Agricultural Sciences, 2015(9):22-25.(in Chinese)
[7]孙毅,崔贵梅,郝曜山,等.超声波辅助花粉介导植物转基因方法[P].中国专利:201110041484.0,2011-02-18.
[2]邹湘辉,庄东红,胡忠,等.负压和超声波处理对农杆菌介导的花生遗传转化效率的影响[J].中国油料作物学报,2004,26(1):12-16.Zou X H, Zhuang D H, Hu Z, et al. Influence of negative pressureand sonication on Agrobact ierium-mediated genetic transforma-tion of peanut(Arachis hypogaea L.)[J]. Chinese Journal of Oil CropSciences, 2004, 26(1):12-16.(in Chinese)
[3]王秀红,白建荣,孙毅,等.花粉介导法将TaPHR1 GFP融合蛋白基因导入玉米[J].西北植物学报,2011,31(9):1732-1737.Wang X H, Bai J R, Sun Y, et al. Transformation of TaPHR1 GFP fusion protein gene into maize by ultrasonicationtreated pollen ap-proach[J]. Acta Bot.Boreal.-Occident.Sin, 2011, 31(9):1732-1737.(in Chinese)
[4] Huang G C, Zhu S W, Dong Y M, et al. Green fluorescent proteinand it s application in plant research[J]. Chinese Bulletin of Botany,1998, 15(5):24-30.
[5] Alesander M, Arkus I M, Diete R H. Stable transformation of sun-flower(Helianthusannuus L.)using an on meristematic regenerationprotocol and green fluorescent protein as avital marker[J]. Transgen-ic Research, 2001, 10:435-444.
[6]南芝润,惠国强,罗绮,等.超声波辅助花粉介导法获得转BtCrylAc基因玉米的研究[J].黑龙江农业科学,2015(9):22-25.Nan Z R, Hui G Q, Luo Q, et al. Research on BtCrylAc transgenicmaize lines using ultrasonic-assisted pollen mediated plant transfor-mation method[J]. Heilongjiang Agricultural Sciences, 2015(9):22-25.(in Chinese)
[7]孙毅,崔贵梅,郝曜山,等.超声波辅助花粉介导植物转基因方法[P].中国专利:201110041484.0,2011-02-18.