多穗柯甜茶乙醇提取物对Neuro-2a细胞分化的影响
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摘要
以Neuro-2a细胞为体外神经分化细胞模型,观察多穗柯乙醇提取物(EELP)对Neuro-2a细胞分化的影响.实验结果表明,EELP可能通过激活Erk1/2和Akt信号通路,显著提高Neuro-2a细胞的分化率,同时促进神经突的生长.该研究可为多穗柯甜茶在神经系统疾病治疗和保健方面的应用提供重要实验依据.
In this study,Neuro-2 a cells were used as an in vitro neural differentiation cell model to observe the effect of ethanol extract of Lithocarpus polystachyrus(EELP) on Neuro-2 a cell differentiation. The results indicate that EELP promotes differentiation and neurite outgrowth of Neuro-2 a cells may be through activating Akt and Erk1/2 signaling pathways. These findings provide an important basis for the application of Lithocarpus polystachyrus Rehd in the treatment and health care of nervous system diseases.
In this study,Neuro-2 a cells were used as an in vitro neural differentiation cell model to observe the effect of ethanol extract of Lithocarpus polystachyrus(EELP) on Neuro-2 a cell differentiation. The results indicate that EELP promotes differentiation and neurite outgrowth of Neuro-2 a cells may be through activating Akt and Erk1/2 signaling pathways. These findings provide an important basis for the application of Lithocarpus polystachyrus Rehd in the treatment and health care of nervous system diseases.
引文
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[2]杨崇仁.天然甜味剂植物资源的开发利用[J].植物学报,1984,2(1):21-23.
[3]何春年,彭勇,肖伟,等.多穗柯甜茶的研究进展[J].时珍国医国药,2012,23(5):1253-1255.
[4]廖晓峰,于荣,肖坤福.天然野生植物多穗柯甜茶的化学成分分析[J].生物质化学工程,2003,37(6):32-34.
[5]董华强,宁正祥,于立静,等.多穗柯黄酮根皮苷对糖尿病小鼠的降血糖血脂效果[J].食品科学,2006,27(12):714-718.
[6]Lu Y,Foo L Y.Antioxidant and radical scavenging activities of polyphenols from apple pomace.Food Chemistry,2000,68(1):81-85.
[7]李胜华,伍贤进,向晓军.多穗柯有效成分的提取及抑菌效果研究[J].食品科技,2010(3):211-214.
[8]Briski K P,Marshall E S.Induction of ependymal,glial,and neuronal transactivation by intraventricular administration of the SGLT1 NA+-D-glucose cotransporter inhibitor phlorizin[J].Neurochemical Research,2001,26(7):783-792.
[9]Guthrie S.Neuronal Development:Putting Motor Neurons in Their Place[J].Current Biology Cb,2004,14(4):166-168.