油茶果壳多糖乙醇提取工艺的优化及其吸湿保湿性能
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摘要
目的优化油茶果壳多糖乙醇提取工艺,并评价其吸湿保湿性能。方法在单因素试验基础上,以乙醇体积分数、料液比、提取时间、提取次数为影响因素,多糖得率为评价指标,正交试验优化提取工艺。然后,以海藻酸钠为对照,在相对湿度43%、81%下测定48 h内吸湿率,在相对湿度34%下测定48 h内保湿率。结果最佳条件为乙醇体积分数60%,粉末粒径80目,提取温度100℃,料液比1∶35,提取时间1.5 h,提取2次,多糖得率5.42%。在相对湿度43%、81%下多糖吸湿率分别为30.5%、50.1%,在相对湿度34%下其保湿率为20.8%,与海藻酸钠接近。结论该方法稳定可行,可用于乙醇提取吸湿保湿性能较好的油茶果壳多糖。
AIM To optimize the ethanol extraction process for polysaccharides from Camellia oleifera Abel. fruit shells and to evaluate their moisture-absorption, moisture-retention properties.METHODS With ethanol concentration, solid-liquid ratio, extraction time and extraction frequency as influencing factors, polysaccharides yield as an evaluation index, the extraction process was optimized by orthogonal test on the basis of single factor test. Subsequently, with sodium alginate as a control, moisture-absorption rates within 48 h under the relative humidities of 43% and 81%, and moisture-retention rate within 48 h under the relative humidity of 34% were determined, respectively.RESULTS The optimal conditions were determined to be 60% for ethanol concentration, 80 mesh for powder particle size, 100 ℃ for extraction temperature, 1∶35 for solid-liquid ratio, 1.5 h for extraction time, and extraction twice, the polysaccharides yield was 5.42%. The polysaccharides demonstrated the moisture-absorption rates of 30.5% and 50.1% under the relative humidities of 43% and 81%, respectively, with the moisture-retention rate of 20.8% under the relative humidity of 34%, which approximated those of sodium alginate.CONCLUSION This stable and feasible method can be used for the ethanol extraction for polysaccharides with good moisture-absorption, moisture-retention properties from C. oleifera fruit shells.
AIM To optimize the ethanol extraction process for polysaccharides from Camellia oleifera Abel. fruit shells and to evaluate their moisture-absorption, moisture-retention properties.METHODS With ethanol concentration, solid-liquid ratio, extraction time and extraction frequency as influencing factors, polysaccharides yield as an evaluation index, the extraction process was optimized by orthogonal test on the basis of single factor test. Subsequently, with sodium alginate as a control, moisture-absorption rates within 48 h under the relative humidities of 43% and 81%, and moisture-retention rate within 48 h under the relative humidity of 34% were determined, respectively.RESULTS The optimal conditions were determined to be 60% for ethanol concentration, 80 mesh for powder particle size, 100 ℃ for extraction temperature, 1∶35 for solid-liquid ratio, 1.5 h for extraction time, and extraction twice, the polysaccharides yield was 5.42%. The polysaccharides demonstrated the moisture-absorption rates of 30.5% and 50.1% under the relative humidities of 43% and 81%, respectively, with the moisture-retention rate of 20.8% under the relative humidity of 34%, which approximated those of sodium alginate.CONCLUSION This stable and feasible method can be used for the ethanol extraction for polysaccharides with good moisture-absorption, moisture-retention properties from C. oleifera fruit shells.
引文
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[3] 范康福,吴雪辉.油茶果壳制备活性炭的工艺研究[J].现代食品科技,2013,29(2):339-341,375.
[4] 朱彬,钟海雁,曹清明,等.油茶活性成分研究进展与展望[J].经济林研究,2010,28(3):140-145.
[5] 康海权,陈亚琪,陈秋平,等.油茶果壳多糖的抗氧化作用及单糖组成[J].食品与发酵工业,2010,36(4):36-39.
[6] 贾艳丽,杜世新,陈文,等.中药桃胶对Ⅱ度烧伤暴露创面的影响[J].基层医学论坛,2008,12(34):1057-1059.
[7] Jin X C.Bioactivities of water-soluble polysaccharides from fruit shell of Camellia oleifera Abel:Antitumor and antioxidant activities[J].Carbohydr Polym,2012,87(3):2198-2201.
[8] 廖永丽,陈景斯,郭华敏,等.不同产地油茶果壳多糖含量的研究[J].广东药科大学学报,2017,33(5):620-623.
[9] 蔡延渠,邓剑壕,朱志东,等.不同产地原桃胶的多糖含量研究[J].广东药科大学学报,2018,34(1):25-28.
[10] 陈向阳,王恒,刘勤,等.正交设计法优化木瓜果实多糖提取工艺[J].安徽农学通报,2018,24(13):18-20.
[11] 石征蓉,谷江华,杨秀青,等.基于多指标权重分析和正交设计法优选糖肾清毒颗粒的提取工艺[J].辽宁中医杂志,2018,45(3):577-582.
[12] 张丕德,龙晓英.正交设计与数据分析在药学研究中的应用(Ⅰ)[J].广东药学院学报,2009,25(5):546-551.
[13] 倪志华,李云凤,徐陞梅.茯苓多糖吸湿保湿性能的研究[J].山东化工,2015,44(21):17-18.
[14] 蔡延渠,董碧莲,邓剑壕,等.改良桃胶多糖的吸湿保湿性能及体外透皮吸收研究[J].广州中医药大学学报,2018,35(4):711-716.
[15] 黄仁术,谷仿丽,何晓梅.金荞麦多糖的吸湿保湿性能研究[J].化工新型材料,2014,42(6):141-142.