外源甜菜碱对低温胁迫下紫花苜蓿幼苗生理特性的影响
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摘要
本研究以‘甘农5号’紫花苜蓿(Medicago sativa L‘Gannong No.5’)品种为实验材料,设置两种施加方式(叶施、根施)和六个外源甜菜碱浓度(0,10,20,30,40和50mmol·L~(-1))处理苜蓿幼苗,在低温(4℃)胁迫7d后测定其生长和相关生理指标,探究外源甜菜碱缓解苜蓿低温胁迫的生理机制。结果表明,叶施和根施外源甜菜碱均能够有效缓解低温胁迫下苜蓿幼苗苗长、根长、地上及地下生物量和叶绿素含量的降低,增加可溶性糖和游离脯氨酸的含量,减少叶片相对膜透性和丙二醛含量的升高,显著提高抗氧化酶活性以及非酶抗氧化物质含量,同时,外源甜菜碱还可以促进低温胁迫下苜蓿幼苗内源甜菜碱的积累和甜菜碱脱氢酶活性,增强渗透调节能力,进而提高苜蓿对低温胁迫的抗性。通过隶属函数分析得出,叶施30mmol·L~(-1)和根施40mmol·L~(-1)的外源甜菜碱对缓解苜蓿幼苗低温胁迫的效果最佳。
In this study,the‘Ganong 5 th'alfalfa(Medicago sativa L.)was used as experimental material,and two application methods(leaf application,root application)and six exogenous betaine concentrations(0,10,20,30,40 and 50 mmol·L~(-1))were used to treat the seedlings of alfalfa.Plant growth and related physiological indexes were measured after 7 days of low temperature(4℃)stress,and the physiological mechanism of exogenous betaine to alleviate the low temperature stress of alfalfa was explored.The results showed that exogenous GB alleviated the loss of plant height,root length,leaf dry weight,root dry weight and chlorophyll content,and increase the content of soluble sugar(SS)and free proline(Pro),and reduced the increase in leaf relative membrane permeability and MDA content.Exogenous GB increased antioxidant enzyme activities and non-enzymatic antioxidant content.And also,exogenous GB enhanced the osmotic adjustment capacity of the plant by increasing endogenous GB accumulation and BADH activity.These results indicated that exogenous GB enhanced the resistance of Medicago sativa to low-temperature stress under low temperature adjustment. The membership function analysis indicated that spraying 30 mmol·L~(-1) GB on the leaves and spraying 40 mmol·L~(-1) GB on the roots had the best effect on alleviatingthe low-temperature stress of alfalfa seedlings.
In this study,the‘Ganong 5 th'alfalfa(Medicago sativa L.)was used as experimental material,and two application methods(leaf application,root application)and six exogenous betaine concentrations(0,10,20,30,40 and 50 mmol·L~(-1))were used to treat the seedlings of alfalfa.Plant growth and related physiological indexes were measured after 7 days of low temperature(4℃)stress,and the physiological mechanism of exogenous betaine to alleviate the low temperature stress of alfalfa was explored.The results showed that exogenous GB alleviated the loss of plant height,root length,leaf dry weight,root dry weight and chlorophyll content,and increase the content of soluble sugar(SS)and free proline(Pro),and reduced the increase in leaf relative membrane permeability and MDA content.Exogenous GB increased antioxidant enzyme activities and non-enzymatic antioxidant content.And also,exogenous GB enhanced the osmotic adjustment capacity of the plant by increasing endogenous GB accumulation and BADH activity.These results indicated that exogenous GB enhanced the resistance of Medicago sativa to low-temperature stress under low temperature adjustment. The membership function analysis indicated that spraying 30 mmol·L~(-1) GB on the leaves and spraying 40 mmol·L~(-1) GB on the roots had the best effect on alleviatingthe low-temperature stress of alfalfa seedlings.
引文
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[13]张文浩,侯龙鱼,杨杰,等.高寒地区苜蓿人工草地建植技术[J].科学通报,2018,63(17):1651-1663
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[23]杨德光,孙玉珺,Ali Raza Irfan,等.低温胁迫对玉米发芽及幼苗生理特性的影响[J].东北农业大学学报,2018,49(5):1-8
[24]陈胜萍,刘晓光,赵成萍,等.低温胁迫对不同番茄品种生长和生理生化指标的影响[J].山西农业大学学报(自然科学版),2017,37(11):780-784
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[26]卫丹丹.低温胁迫下甜菜碱对番茄叶片光合作用的保护机制[D].泰安:山东农业大学,2016:35-41
[27]戴玉池,黄亮,陈良碧.5种抗寒剂对早稻相早籼25号幼苗的耐寒生理鉴定及其利用[J].湖南师范大学自然科学学报,2000,23(3):88-94
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[32]惠红霞,许兴,李前荣.外源甜菜碱对盐胁迫下枸杞光合功能的改善[J].西北植物学报,2003,23(12):2137-2422
[33]赵新西,马千全,杨兴洪,等.根施甜菜碱对干旱胁迫下小麦幼苗类囊体膜组分和功能的影响[J].植物生理与分子生物学学报,2005,31(2):135-142
[34]梁小红,安勐颍,宋峥,等.外源甜菜碱对低温胁迫下结缕草生理特性的影响[J].草业学报,2015,24(9):181-188
[35]张翠平,王艳慧,袁小环,等.钼酸钠对低温胁迫下狼尾草渗透调节物质和膜稳定性的影响[J].草业科学,2012,29(8):1245-1249
[36]徐锦海.根施不同浓度甜菜碱对水稻幼苗抗冷性的影响[J].安徽农业大学学报,2007,34(4):491-495
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