一测多评法测定蜂胶类保健食品中咖啡酸等4种成分的含量
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摘要
目的:建立以蜂胶为单一原料的保健食品中咖啡酸、阿魏酸、异阿魏酸及咖啡酸苯乙酯的"一测多评"含量测定方法。方法:采用高效液相色谱法,以甲醇-0.1%磷酸水溶液为流动相,梯度洗脱,以异阿魏酸为参照物,建立其与咖啡酸、阿魏酸,咖啡酸苯乙酯的相对校正因子,利用相对校正因子计算4种成分的含量,实现一测多评;并对多批样品同时采用一测多评法和标准曲线法测定,将结果进行比较,验证方法的可行性。结果:4种成分在相应的线性范围内线性关系良好,相关系数r>0.9999,平均加标回收率在99.85%～101.69%,RSD在1.7%～2.3%,咖啡酸、阿魏酸、咖啡酸苯乙酯的相对校正因子分别为0.9503、0.9276、1.3902,且在不同实验条件下重现性良好,一测多评法计算值与标准曲线法实测值相对平均偏差在0.1%～1.7%之间,测定结果基本一致。结论:本文建立的一测多评方法操作简便,重复性好,可为以蜂胶为单一原料的保健食品的质量评价提供参考。
Objective:To establish a quantitative analysis of multi-components by single-maker(QAMS)method for the determination of caffeic acid,erulic acid,isoferulic acid and caffeic acid phenylethyl ester in health food with propolis as a single raw material. Methods:HPLC was used with methanol-0.1% phosphoric acid aqueous solution as mobile phase,gradient elution and isoferulic acid as reference substance to establish its relative correction factors with caffeic acid,ferulic acid and caffeic acid phenylethyl ester. Relative correction factor was used to calculate the content of four components and the method of"QAMS"realized. The method of "QAMS" and standard curve were used to compare the results and verify the feasibility of the method. Results:Within a certain linear range,4 components had good linear relationships,r>0.9999.The average recovery rates were in the rage of 99.85%～101.69%,and the relative standard deviations(RSDs)were in the range of 1.7%～2.3%. The relative correction factors(RCFs)with caffeic acid,erulic acid and caffeic acid phenylethyl ester with reference to isoferulic acid were 0.9503,0.9276,1.3902,respectively. The relative correction factors of each component had good reproducibility under different experimental conditions. There was almost no difference between the calculated values of "QAMS" method and the measured values of standard curve method,and the relative average deviations were in the range of 0.1%～1.7%.Conclusion:The method of "QAMS" was simple and reproducible,and could provide reference for the quality evaluation of health food with propolis as a single raw material.
Objective:To establish a quantitative analysis of multi-components by single-maker(QAMS)method for the determination of caffeic acid,erulic acid,isoferulic acid and caffeic acid phenylethyl ester in health food with propolis as a single raw material. Methods:HPLC was used with methanol-0.1% phosphoric acid aqueous solution as mobile phase,gradient elution and isoferulic acid as reference substance to establish its relative correction factors with caffeic acid,ferulic acid and caffeic acid phenylethyl ester. Relative correction factor was used to calculate the content of four components and the method of"QAMS"realized. The method of "QAMS" and standard curve were used to compare the results and verify the feasibility of the method. Results:Within a certain linear range,4 components had good linear relationships,r>0.9999.The average recovery rates were in the rage of 99.85%～101.69%,and the relative standard deviations(RSDs)were in the range of 1.7%～2.3%. The relative correction factors(RCFs)with caffeic acid,erulic acid and caffeic acid phenylethyl ester with reference to isoferulic acid were 0.9503,0.9276,1.3902,respectively. The relative correction factors of each component had good reproducibility under different experimental conditions. There was almost no difference between the calculated values of "QAMS" method and the measured values of standard curve method,and the relative average deviations were in the range of 0.1%～1.7%.Conclusion:The method of "QAMS" was simple and reproducible,and could provide reference for the quality evaluation of health food with propolis as a single raw material.
引文
[1]国家药典委员会主编.中共人民共和国药典 2015年版[M].北京:中国医药科技出版社,2015:358-359.
[2]罗照明,张红城.中国蜂胶化学成分及其生物活性的研究[J].中国蜂业,2012,63(Z2):55-62.
[3]欧仕益,包惠燕,蓝志东.阿魏酸及其衍生物的药理作用研究进展[J].中药材,2001,24(3):220-221.
[4]潘瑞乐,陈迪华,沈连刚,等.高效液相色谱法测定中药升麻中阿魏酸和异阿魏酸的含量[J].药物分析杂志,2000,20(6):396-398.
[5]张强,胡志力,王福文,等.咖啡酸对阿糖胞苷致小鼠白细胞、血小板减少及血小板体积变化的预防和治疗作用[J].中国临床药理学与治疗学,2008,13(5):508-511.
[6]玄红专,胡福良,顾美儿.蜂胶中咖啡酸苯乙酯的研究进展[J].食品研究与开发,2006,27(5):14-15.
[7]李熠,赵静,薛晓锋,等.超高效液相色谱法同时测定蜂胶中的12种活性成分[J].色谱,2007,25(6):857-860.
[8]于世锋,符军放,陈卫军,等.高效液相色谱法测定蜂胶中4种酚酸的含量[J].中国蜂业,2007,58(9):8-10.
[9]吴毅,吴良发,许妍,等.HPLC 法同时测定蜂胶软胶囊中8种黄酮的含量[J].中药材,2013,36(2):314-316.
[10]国家药典委员会主编.中共人民共和国药典 2015年版[M].北京:中国医药科技出版社,2015:76-77.
[11]国家药典委员会主编.中共人民共和国药典 2015年版[M].2015年版.北京:中国医药科技出版社,2015:101.
[12]国家药典委员会主编.中共人民共和国药典 2015年版[M].2015年版.北京:中国医药科技出版社,2015:416-417.
[13]国家药典委员会主编.中共人民共和国药典 2015年版[M].2015年版.北京:中国医药科技出版社,2015:1182-1183.
[14]李木子,王京辉,郭洪祝,等.HPLC法测定白术饮片中多种化学成分的含量[J].药物分析杂志,2017,37(9):1585-1592.
[15]赵倩,冯伟红,张启伟,等.“一测多评”法用于栀子金花丸多成分含量测定研究的可行性研究[J].中国中药杂志,2014,39(10):1826-1833.
[16]翟宏宇,单柏宇,王海洋,等.一测多评法测定保心宁片中6个丹参类指标性成分的含量[J].药物分析杂志,2018,38(6):973-978.
[17]闫丹,江敏瑜,王云红,等.一测多评法在玄参药材质量控制中的应用[J].中草药,2018,49(20):4892-4898.
[18]魏丽娟,易倩,张曲,等.一测多评法测定藜麦中6种酚类成分[J].食品工业科技,2018,39(19):232-236,242.
[19]王智民,钱忠直,张启伟,等.一测多评法建立的技术指南[J].中国中药杂志,2011,36(6):657.
[20]何兵,杨世艳,张燕.一测多评中待测成分校正和定位的新方法研究[J].药学学报,2012,47(12):1653-1659.
[2]罗照明,张红城.中国蜂胶化学成分及其生物活性的研究[J].中国蜂业,2012,63(Z2):55-62.
[3]欧仕益,包惠燕,蓝志东.阿魏酸及其衍生物的药理作用研究进展[J].中药材,2001,24(3):220-221.
[4]潘瑞乐,陈迪华,沈连刚,等.高效液相色谱法测定中药升麻中阿魏酸和异阿魏酸的含量[J].药物分析杂志,2000,20(6):396-398.
[5]张强,胡志力,王福文,等.咖啡酸对阿糖胞苷致小鼠白细胞、血小板减少及血小板体积变化的预防和治疗作用[J].中国临床药理学与治疗学,2008,13(5):508-511.
[6]玄红专,胡福良,顾美儿.蜂胶中咖啡酸苯乙酯的研究进展[J].食品研究与开发,2006,27(5):14-15.
[7]李熠,赵静,薛晓锋,等.超高效液相色谱法同时测定蜂胶中的12种活性成分[J].色谱,2007,25(6):857-860.
[8]于世锋,符军放,陈卫军,等.高效液相色谱法测定蜂胶中4种酚酸的含量[J].中国蜂业,2007,58(9):8-10.
[9]吴毅,吴良发,许妍,等.HPLC 法同时测定蜂胶软胶囊中8种黄酮的含量[J].中药材,2013,36(2):314-316.
[10]国家药典委员会主编.中共人民共和国药典 2015年版[M].北京:中国医药科技出版社,2015:76-77.
[11]国家药典委员会主编.中共人民共和国药典 2015年版[M].2015年版.北京:中国医药科技出版社,2015:101.
[12]国家药典委员会主编.中共人民共和国药典 2015年版[M].2015年版.北京:中国医药科技出版社,2015:416-417.
[13]国家药典委员会主编.中共人民共和国药典 2015年版[M].2015年版.北京:中国医药科技出版社,2015:1182-1183.
[14]李木子,王京辉,郭洪祝,等.HPLC法测定白术饮片中多种化学成分的含量[J].药物分析杂志,2017,37(9):1585-1592.
[15]赵倩,冯伟红,张启伟,等.“一测多评”法用于栀子金花丸多成分含量测定研究的可行性研究[J].中国中药杂志,2014,39(10):1826-1833.
[16]翟宏宇,单柏宇,王海洋,等.一测多评法测定保心宁片中6个丹参类指标性成分的含量[J].药物分析杂志,2018,38(6):973-978.
[17]闫丹,江敏瑜,王云红,等.一测多评法在玄参药材质量控制中的应用[J].中草药,2018,49(20):4892-4898.
[18]魏丽娟,易倩,张曲,等.一测多评法测定藜麦中6种酚类成分[J].食品工业科技,2018,39(19):232-236,242.
[19]王智民,钱忠直,张启伟,等.一测多评法建立的技术指南[J].中国中药杂志,2011,36(6):657.
[20]何兵,杨世艳,张燕.一测多评中待测成分校正和定位的新方法研究[J].药学学报,2012,47(12):1653-1659.