松花粉的主要活性成分及其功能的研究进展
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摘要
松花粉为松科植物马尾松(Pinus massoniana Lamb.)、油松(Pinus tabuli formis Carr.)或同属数种植物的干燥花粉,是一种药食同源的中药材,具有多种活性成分及保健功效。近年来松花粉中活性物质组成及其功能特性成为科研工作者的研究重点。该文对松花粉中植物甾醇、黄酮、多糖等主要活性物质的提取和成分表征以及活性物质抗疲劳、抗氧化、保护肝脏和心血管等主要功能进行了综述,为进一步的理论和应用研究提供依据。
Pine pollen is dried pollen of Pinus massoniana Lamb.,Pinus tabuli formis Carr.,or plants of the same genus. Pine pollen as edible and medicinal material,which contains many active ingredients and health benefits. At present,the research on pine pollen mainly focuses on its active ingredients and basic functions. It summarized the extraction and composition characterization of phytosterols,flavonoids,polysaccharides and other active substances in pine pollen as well as the main functions of active substances such as anti-fatigue,antioxidation,protection of liver and cardiovascular,etc,which provides the basis for further theoretical and applied research.
Pine pollen is dried pollen of Pinus massoniana Lamb.,Pinus tabuli formis Carr.,or plants of the same genus. Pine pollen as edible and medicinal material,which contains many active ingredients and health benefits. At present,the research on pine pollen mainly focuses on its active ingredients and basic functions. It summarized the extraction and composition characterization of phytosterols,flavonoids,polysaccharides and other active substances in pine pollen as well as the main functions of active substances such as anti-fatigue,antioxidation,protection of liver and cardiovascular,etc,which provides the basis for further theoretical and applied research.
引文
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[19]李守汉,廉永善,李洁.破壁松花粉对运动大鼠免疫功能及运动能力的影响[J].营养学报,2016,38(2):201-202
[20]刘协,胡启之,李小宁,等.松花粉的抗疲劳作用研究[J].中国生化药物杂志,2004,25(3):169-170
[21]谢惠萍,陈琼瑶,蒋中仁,等.松花粉对酒精性肝损伤的保护功能研究[J].中国现代医药杂志,2008,10(4):47-49
[22]朱良,裴植仁.松花粉总黄酮对四氯化碳所致小鼠急性肝损伤的保护作用[J].时珍国医国药,2010,21(8):1903-1904
[23]李雯雯,陈才法,李悦,等.松花粉醇提物抗肿瘤作用的初步研究[J].解放军药学学报,2011,27(3):199-201
[24]李艳艳,郑萍.蒽酮-硫酸比色法测定松花粉中粗多糖的含量[J].云南师范大学学报,2017,37(4):58-63
[25]袁桂香,罗小菊,刘映良.马尾松松花粉多糖提取工艺[J].林业工程学报,2016,1(4):80-84
[26]Li S,Yang G,Yan J,et al.Polysaccharide structure and immunological relationships of RG-I pectin from the bee pollen of Nelumbo nucifera[J].International Journal of Biological Macromolecules,2018,111:660-666
[27]Yang X,Guo D,Zhang J,et al.Characterization and anti-tumor activity of pollen polysaccharide[J].International Immunopharmacology,2007,7(3):401-407
[28]Chu H L,Mao H,Feng W,et al.Effects of sulfated polysaccharide from Masson pine pollen on the proliferation and cell cycle of HepG2 cells[J].International Journal of Biological Macromolecules,2013,55(3):104-108
[29]Cong T,Jin X Y,Zhao L,et al.Anti-fibrotic effects of the Masson pine pollen aqueous extract on hepatic fibrosis rat model[J].International Journal of Clinical&Experimental Pathology,2015,8(5):46-51
[30]高振.马尾松花粉多糖的结构分析以及硫酸酯化多糖对肝癌细胞HepG2的影响[D].济南:山东师范大学,2017
[31]Guo F,Xue C,Wu C,et al.Immunoregulatory effects of Taishan Pinus massoniana,pollen polysaccharide on chicks co-infected with avian leukosis,virus and Bordetella avium,early in ovo[J].Research in Veterinary Science,2014,96(2):260-266
[32]Li B,Wei K,Yang S,et al.Immunomodulatory effects of Taishan Pinus massoniana pollen polysaccharide and propolis on immunosuppressed chickens[J].Microbial Pathogenesis,2015,78:7-13
[33]Geng Y,Xing L,Sun M,et al.Immunomodulatory effects of sulfated polysaccharides of pine pollen on mouse macrophages[J].International Journal of Biological Macromolecules,2016,91:846-855
[34]刘月冉,冯潍,耿越.马尾松花粉酯化多糖对MIN6细胞胰岛素分泌和[Ca2+]i的影响[J].药物生物技术,2013(2):137-140
[35]冯潍.松花粉硫酸酯化多糖影响T2MD小鼠血糖调节机制的初步研究[D].济南:山东师范大学,2013
[36]高爱新,王舟莲,王敬文,等.超临界CO2萃取技术提取松花粉中植物甾醇的研究[J].食品科技,2010(4):208-210
[37]Wu Xiao-Qin,Liu J E,Jin C,et al.Study on content of fundamental components and secondary metabolites in different kinds of pine pollen[J].Science&Technology of Food Industry,2011,32(1):273-276
[38]Huang L,Zhong T,Chen T,et al.Identification of beta-sitosterol,stigmasterol and ergosterin in A.roxburghii using supercritical fluid extraction followed by liquid chromatography/atmospheric pressure chemical ionization ion trap mass spectrometry[J].Rapid Communications in Mass Spectrometry,2010,21(18):3024-3032
[39]孙阳恩,钤莉妍,张含,等.马尾松花粉中植物甾醇成分分析[J].天然产物研究与开发,2012,24(12):1753-1756
[40]朱宝安,张军要.松花粉总甾醇对大鼠前列腺良性增生的影响[J].现代预防医学,2016,43(4):623-626
[41]Wang R,Kobayashi Y,Lin Y,et al.A phytosterol enriched refined extract of Brassica campestris L.pollen significantly improves benign prostatic hyperplasia(BPH)in a rat model as compared to the classical TCM pollen preparation Qianlie Kang Pule'an Tablets[J].Phytomedicine International Journal of Phytotherapy&Phytopharmacology,2015,22(1):145-152
[42]彭修娟,杨志杰,刘半仙.秦岭松花粉治疗小鼠前列腺增生实验研究[J].河南中医,2014,34(6):1048-1049
[43]Fassbender K,Lütjohann D,Dik M G,et al.Moderately elevated plant sterol levels are associated with reduced cardiovascular risk--the LASA study[J].Atherosclerosis,2008,196(1):283-288
[44]Dhankhar J.Cardioprotective effects of phytosterols[J].International Journal of Pharmaceutical Sciences&Research,2013,4(2):590-596
[45]García-Llatas G,Rodríguez-Estrada M T.Current and new insights on phytosterol oxides in plant sterol-enriched food[J].Chemistry&Physics of Lipids,2011,164(6):607-624
[46]刘杰尔.松花粉成分分析及其轻身减肥功能初探[D].杭州:浙江大学,2008
[47]许继敏.松花粉甾醇对3T3-F442A前脂肪细胞增殖、分化及糖代谢的影响[D].济南:山东师范大学,2014
[48]Mirasmoreno B,Sabaterjara A B,Pedreno M A,et al.Bioactivity of phytosterols and their production in plant in vitro cultures[J].Journal of Agricultural&Food Chemistry,2016,64(38):7049-7058
[49]Misawa E,Tanaka M,Nomaguchi K,et al.Oral Ingestion of Aloe vera Phytosterols Alters Hepatic Gene Expression Profiles and Ameliorates Obesity-Associated Metabolic Disorders in Zucker Diabetic Fatty Rats[J].J Agric Food Chem,2012,60(11):2799-2806
[50]Kasmi K C E,Anderson A L,Devereaux M W,et al.Phytosterols Promote Liver Injury and Kupffer Cell Activation in Parenteral Nutrition-Associated Liver Disease[J].Science Translational Medicine,2013,206(5):137-152
[51]李丽.马尾松花粉的化学成分及其对胃粘膜的保护作用[D].沈阳:辽宁中医药大学,2010
[52]董素哲.松花粉的质量评价方法的研究[D].成都:成都中医药大学,2012
[53]宣自华,余进海,王学林,等.富铬松花粉对非酒精性脂肪肝大鼠的影响[J].中药材,2014,37(8):1445-1448
[54]Luo Y,Wei Y,Wang T,et al.Pine pollen inhibits cell apoptosis-related protein expression in the cerebral cortex of mice with arsenic poisoning[J].Neural Regeneration Research,2012,7(12):896-899
[2]赖桂萍,杨继红,詹小涛.松花粉药理作用及临床应用研究概况[J].中国实用医药,2011,6(28):248-251
[3]支崇远,王开发.四种松花粉营养成分比较研究[J].中国自然医学杂志,2004,6(2):81-83
[4]沈德嫱,陈立勇.松花粉的保健功效及其临床应用研究进展[J].中国民族民间医药,2017,26(6):55-57
[5]何晓燕,孙雪圆,于智洋.松花粉的有效成分及药理作用[J].东北林业大学学报,2007,35(9):78-80
[6]邓倩,童珊珊,丁丽霞,等.松花粉活性成分分析方法及药理作用的研究进展[J].药物分析杂志,2012(1):173-178
[7]侯晨晨,沈蓉,刘迪,等.松花粉药理作用及临床应用研究进展[J].中国中医药信息杂志,2017,24(9):124-127
[8]李凤林,李青旺,冯彩宁,等.天然黄酮类化合物提取方法研究进展[J].中国食品添加剂,2008(5):60-64
[9]郭丽梅,王盼盼,杨蒙,等.油松花粉黄酮的提取及黄酮酒的制备[J].天津科技大学学报,2013(4):10-13
[10]李燕梅.乙醇浸泡对松花粉破壁影响初探[J].轻工科技,2018(1):32-33
[11]邵圣娟,卫静莉.聚乙二醇-硫酸铵双水相体系萃取松花粉中总黄酮的工艺研究[J].食品工业科技,2017,38(11):266-269
[12]王晖,耿越,徐宏楠,等.松花粉中非营养生物活性物质研究[J].食品研究与开发,2016,37(21):208-211
[13]刘嘉宝,范国栋,冯武.云南松花粉中黄酮类成分研究初报[J].西部林业科学,2001(2):67-70
[14]王丹丹.三种植物提取物对H2O2诱导PC12细胞氧化损伤的保护作用研究[D].济南:山东师范大学,2016
[15]Wang S W,Wang Y J,Su Y J,et al.Rutin inhibits-amyloid aggregation and cytotoxicity,attenuates oxidative stress,and decreases the production of nitric oxide and proinflammatory cytokines[J].Neurotoxicology,2012,33(3):482-490
[16]回晶,林家帅,李天翼,等.松花粉黄酮提取物体外抗氧化作用的研究[J].辽宁大学学报(自然科学版),2014,41(3):281-285
[17]董素哲,裴瑾,刘薇,等.松花粉抗氧化活性与主要成分的关联分析[J].中药与临床,2012,3(3):5-7
[18]胡水保,李海龙,陈琳婉,等.玛咖松花粉合剂抗疲劳作用的研究[J].海峡药学,2014(6):42-44
[19]李守汉,廉永善,李洁.破壁松花粉对运动大鼠免疫功能及运动能力的影响[J].营养学报,2016,38(2):201-202
[20]刘协,胡启之,李小宁,等.松花粉的抗疲劳作用研究[J].中国生化药物杂志,2004,25(3):169-170
[21]谢惠萍,陈琼瑶,蒋中仁,等.松花粉对酒精性肝损伤的保护功能研究[J].中国现代医药杂志,2008,10(4):47-49
[22]朱良,裴植仁.松花粉总黄酮对四氯化碳所致小鼠急性肝损伤的保护作用[J].时珍国医国药,2010,21(8):1903-1904
[23]李雯雯,陈才法,李悦,等.松花粉醇提物抗肿瘤作用的初步研究[J].解放军药学学报,2011,27(3):199-201
[24]李艳艳,郑萍.蒽酮-硫酸比色法测定松花粉中粗多糖的含量[J].云南师范大学学报,2017,37(4):58-63
[25]袁桂香,罗小菊,刘映良.马尾松松花粉多糖提取工艺[J].林业工程学报,2016,1(4):80-84
[26]Li S,Yang G,Yan J,et al.Polysaccharide structure and immunological relationships of RG-I pectin from the bee pollen of Nelumbo nucifera[J].International Journal of Biological Macromolecules,2018,111:660-666
[27]Yang X,Guo D,Zhang J,et al.Characterization and anti-tumor activity of pollen polysaccharide[J].International Immunopharmacology,2007,7(3):401-407
[28]Chu H L,Mao H,Feng W,et al.Effects of sulfated polysaccharide from Masson pine pollen on the proliferation and cell cycle of HepG2 cells[J].International Journal of Biological Macromolecules,2013,55(3):104-108
[29]Cong T,Jin X Y,Zhao L,et al.Anti-fibrotic effects of the Masson pine pollen aqueous extract on hepatic fibrosis rat model[J].International Journal of Clinical&Experimental Pathology,2015,8(5):46-51
[30]高振.马尾松花粉多糖的结构分析以及硫酸酯化多糖对肝癌细胞HepG2的影响[D].济南:山东师范大学,2017
[31]Guo F,Xue C,Wu C,et al.Immunoregulatory effects of Taishan Pinus massoniana,pollen polysaccharide on chicks co-infected with avian leukosis,virus and Bordetella avium,early in ovo[J].Research in Veterinary Science,2014,96(2):260-266
[32]Li B,Wei K,Yang S,et al.Immunomodulatory effects of Taishan Pinus massoniana pollen polysaccharide and propolis on immunosuppressed chickens[J].Microbial Pathogenesis,2015,78:7-13
[33]Geng Y,Xing L,Sun M,et al.Immunomodulatory effects of sulfated polysaccharides of pine pollen on mouse macrophages[J].International Journal of Biological Macromolecules,2016,91:846-855
[34]刘月冉,冯潍,耿越.马尾松花粉酯化多糖对MIN6细胞胰岛素分泌和[Ca2+]i的影响[J].药物生物技术,2013(2):137-140
[35]冯潍.松花粉硫酸酯化多糖影响T2MD小鼠血糖调节机制的初步研究[D].济南:山东师范大学,2013
[36]高爱新,王舟莲,王敬文,等.超临界CO2萃取技术提取松花粉中植物甾醇的研究[J].食品科技,2010(4):208-210
[37]Wu Xiao-Qin,Liu J E,Jin C,et al.Study on content of fundamental components and secondary metabolites in different kinds of pine pollen[J].Science&Technology of Food Industry,2011,32(1):273-276
[38]Huang L,Zhong T,Chen T,et al.Identification of beta-sitosterol,stigmasterol and ergosterin in A.roxburghii using supercritical fluid extraction followed by liquid chromatography/atmospheric pressure chemical ionization ion trap mass spectrometry[J].Rapid Communications in Mass Spectrometry,2010,21(18):3024-3032
[39]孙阳恩,钤莉妍,张含,等.马尾松花粉中植物甾醇成分分析[J].天然产物研究与开发,2012,24(12):1753-1756
[40]朱宝安,张军要.松花粉总甾醇对大鼠前列腺良性增生的影响[J].现代预防医学,2016,43(4):623-626
[41]Wang R,Kobayashi Y,Lin Y,et al.A phytosterol enriched refined extract of Brassica campestris L.pollen significantly improves benign prostatic hyperplasia(BPH)in a rat model as compared to the classical TCM pollen preparation Qianlie Kang Pule'an Tablets[J].Phytomedicine International Journal of Phytotherapy&Phytopharmacology,2015,22(1):145-152
[42]彭修娟,杨志杰,刘半仙.秦岭松花粉治疗小鼠前列腺增生实验研究[J].河南中医,2014,34(6):1048-1049
[43]Fassbender K,Lütjohann D,Dik M G,et al.Moderately elevated plant sterol levels are associated with reduced cardiovascular risk--the LASA study[J].Atherosclerosis,2008,196(1):283-288
[44]Dhankhar J.Cardioprotective effects of phytosterols[J].International Journal of Pharmaceutical Sciences&Research,2013,4(2):590-596
[45]García-Llatas G,Rodríguez-Estrada M T.Current and new insights on phytosterol oxides in plant sterol-enriched food[J].Chemistry&Physics of Lipids,2011,164(6):607-624
[46]刘杰尔.松花粉成分分析及其轻身减肥功能初探[D].杭州:浙江大学,2008
[47]许继敏.松花粉甾醇对3T3-F442A前脂肪细胞增殖、分化及糖代谢的影响[D].济南:山东师范大学,2014
[48]Mirasmoreno B,Sabaterjara A B,Pedreno M A,et al.Bioactivity of phytosterols and their production in plant in vitro cultures[J].Journal of Agricultural&Food Chemistry,2016,64(38):7049-7058
[49]Misawa E,Tanaka M,Nomaguchi K,et al.Oral Ingestion of Aloe vera Phytosterols Alters Hepatic Gene Expression Profiles and Ameliorates Obesity-Associated Metabolic Disorders in Zucker Diabetic Fatty Rats[J].J Agric Food Chem,2012,60(11):2799-2806
[50]Kasmi K C E,Anderson A L,Devereaux M W,et al.Phytosterols Promote Liver Injury and Kupffer Cell Activation in Parenteral Nutrition-Associated Liver Disease[J].Science Translational Medicine,2013,206(5):137-152
[51]李丽.马尾松花粉的化学成分及其对胃粘膜的保护作用[D].沈阳:辽宁中医药大学,2010
[52]董素哲.松花粉的质量评价方法的研究[D].成都:成都中医药大学,2012
[53]宣自华,余进海,王学林,等.富铬松花粉对非酒精性脂肪肝大鼠的影响[J].中药材,2014,37(8):1445-1448
[54]Luo Y,Wei Y,Wang T,et al.Pine pollen inhibits cell apoptosis-related protein expression in the cerebral cortex of mice with arsenic poisoning[J].Neural Regeneration Research,2012,7(12):896-899