低场-高场核磁联用分析缔合态对平肺方中芒果苷提取分离的影响
|
摘要
目的采用低场-高场核磁联用技术探索缔合态对平肺方中芒果苷提取、分离行为的影响。方法以1-脱氧野尻毒霉素(DNJ)-柠檬酸成分为对照,以氢T_2弛豫时间及氢化学位移值的变化为指标,采用低场核磁分析缔合态氢的弛豫特征,结合高场核磁技术验证缔合物结构。结果 DNJ-芒果苷在溶液中以缔合态的形式存在,相对分子质量增大,从而抑制了成分从药材向溶液的迁移,透过率下降,图谱中T_2峰向左偏移,高场核磁中化学位移也发生变化。结论阐明了成分存在状态对芒果苷提取分离行为的影响机制,为研究中药复方煎煮方法及机制探索提供了技术支撑。
Objective To study the effects of association complexes on extraction and separation behavior of mangiferin in Pingfei Decoction by low-field and high-field NMR. Methods Using the T_2 relaxation time and chemical shifts value of hydrogen as index,and group DNJ-citric acid as control, the relaxation characteristrics of hydrogen in association state was analyzed by low field NMR to verify the structure of association complexes combined with high-field NMR. Results DNJ and mangiferin existed in the form of association state with larger molecular weight, which inhibited the migration of components from medicinal materials to solution and caused lower transmittance. And the T_2 peak shifted to the left in the spectrum, and the chemical shift in the high field nuclear magnetic field also changed. Conclusion This experiment clarified the mechanism of the effect of presence of components on extraction and separation behavior of mangiferin and provided technical support for studying the decocting method and mechanism of Chinese herbal compound.
Objective To study the effects of association complexes on extraction and separation behavior of mangiferin in Pingfei Decoction by low-field and high-field NMR. Methods Using the T_2 relaxation time and chemical shifts value of hydrogen as index,and group DNJ-citric acid as control, the relaxation characteristrics of hydrogen in association state was analyzed by low field NMR to verify the structure of association complexes combined with high-field NMR. Results DNJ and mangiferin existed in the form of association state with larger molecular weight, which inhibited the migration of components from medicinal materials to solution and caused lower transmittance. And the T_2 peak shifted to the left in the spectrum, and the chemical shift in the high field nuclear magnetic field also changed. Conclusion This experiment clarified the mechanism of the effect of presence of components on extraction and separation behavior of mangiferin and provided technical support for studying the decocting method and mechanism of Chinese herbal compound.
引文
[1]崔景朝,赵自明.中药配方颗粒研究进展(II)--中药单煎与合煎对比研究概况[J].中国实验方剂学杂志,2011,17(4):240-245.
[2]邓轶渊,高文远,陈海霞,等.中药复方合煎与分煎的差异性研究进展[J].中草药,2005,36(12):1909-1911.
[3]彭国平.中药制药化学[M].北京:中国中医药出版社,2016.
[4]姜苏,陈亚军,郑云枫,等.双参平肺方中药味配伍对知母芒果苷提取率的影响与成分作用探讨[J].南京中医药大学学报,2016,32(6):581-584.
[5]Shao J H,Deng Y M,Song L,et al.Investigation the effects of protein hydration states on the mobility water and fat in meat batters by LF-NMR technique[J].LWT-Food Sci Technol,2016,66:1-6.
[6]段文娟,李月,崔莉,等.低场核磁共振及成像技术分析白芍炮制过程中水分变化规律[J].中国中药杂志,2017,42(11):2092-2096.
[7]Fundo J F,Amaro A L,Madureira A R,et al.Fresh-cut melon quality during storage:An NMR study of water transverse relaxation time[J].J Food Eng,2015,167:71-76.
[8]邓树强,陈彩瑜.HPLC法测定知柏地黄丸中芒果苷的含量[J].中国药房,2013,24(32):3054-3055.
[9]李存玉.超微孔过滤原理的研究与应用[D].南京:南京中医药大学,2013.
[10]Fullerton G D,Potter J L,Dornbluth N C.NMRrelaxation of protons in tissues and other macromolecular water solutions[J].Magn Reson Imag,1982,1(4):209-226.
[11]栗进才,黄鹏.天然产物白藜芦醇的1H-NMR图谱中顺反式结构分析[J].广州化工,2016,44(3):109-110.
[12]蓝蓉,陶炳.分子动力学模拟研究温度对甲醇缔合体系中氢键和弱氢键作用的影响[J].中南民族大学学报:自然科学版,2008,27(1):1-4.
[13]史晗,常宗明,尹花,等.高浓酿造后稀释啤酒中氢键缔合体系的初步研究[J].食品与发酵工业,2013,39(11):6-11.
[2]邓轶渊,高文远,陈海霞,等.中药复方合煎与分煎的差异性研究进展[J].中草药,2005,36(12):1909-1911.
[3]彭国平.中药制药化学[M].北京:中国中医药出版社,2016.
[4]姜苏,陈亚军,郑云枫,等.双参平肺方中药味配伍对知母芒果苷提取率的影响与成分作用探讨[J].南京中医药大学学报,2016,32(6):581-584.
[5]Shao J H,Deng Y M,Song L,et al.Investigation the effects of protein hydration states on the mobility water and fat in meat batters by LF-NMR technique[J].LWT-Food Sci Technol,2016,66:1-6.
[6]段文娟,李月,崔莉,等.低场核磁共振及成像技术分析白芍炮制过程中水分变化规律[J].中国中药杂志,2017,42(11):2092-2096.
[7]Fundo J F,Amaro A L,Madureira A R,et al.Fresh-cut melon quality during storage:An NMR study of water transverse relaxation time[J].J Food Eng,2015,167:71-76.
[8]邓树强,陈彩瑜.HPLC法测定知柏地黄丸中芒果苷的含量[J].中国药房,2013,24(32):3054-3055.
[9]李存玉.超微孔过滤原理的研究与应用[D].南京:南京中医药大学,2013.
[10]Fullerton G D,Potter J L,Dornbluth N C.NMRrelaxation of protons in tissues and other macromolecular water solutions[J].Magn Reson Imag,1982,1(4):209-226.
[11]栗进才,黄鹏.天然产物白藜芦醇的1H-NMR图谱中顺反式结构分析[J].广州化工,2016,44(3):109-110.
[12]蓝蓉,陶炳.分子动力学模拟研究温度对甲醇缔合体系中氢键和弱氢键作用的影响[J].中南民族大学学报:自然科学版,2008,27(1):1-4.
[13]史晗,常宗明,尹花,等.高浓酿造后稀释啤酒中氢键缔合体系的初步研究[J].食品与发酵工业,2013,39(11):6-11.