马氏珠母贝(Pinctada fucata martensii)近交家系与其杂交子代DNA甲基化差异分析
|
摘要
2012年4月,利用马氏珠母贝全同胞家系进行家系内与家系间交配组合构建了近交家系(A与D)与杂交家系(B与C)。贝龄2龄时,比较家系生长性状差异,利用甲基化敏感多态性扩增技术(MSAP)检测了4个家系甲基化水平的差异,估计了甲基化比例与生长性状平均值相关性系数。结果表明,家系杂交子代的平均壳长、平均壳高、平均壳宽与平均体重均值大于近交家系,性状优势率为8.5%~24.7%;近交家系与杂交家系的总条带数和甲基化比例均存在显著性差异(p<0.05)。近交家系A和D获得的总条带数分别729.0±19.6与717.3±28.2,甲基化水平分别为(10.0±0.9)%与(8.5±0.4)%;杂交家系B和C获得的总条带数分别为703.0±10.7与726.3±18.8,甲基化比例分别为(5.9±0.7)%与(5.7±0.9)%;家系的平均壳长、平均壳高、平均壳宽与平均体重性状与甲基化均存在明显负相关,相关系数分别为-0.849、-0.751、-0.674和-0.652。
In April of 2012,inbred families(A and D) and hybrid families(B and C) were constructed by two full-sib families of pearl oyster Pinctada fucata martensii.At the age of 2 years,the differences in growth traits of the four families were compared.The methylation levels' differentiation of the four families was evaluated by methylation-sensitive amplification polymorphism(MSAP) technology.The correlation between the methylation ratio and mean values of growth trait were estimated.The results showed that the mean values of shell length,height,width and total weight of hybrid families were larger than inbred families.Heterosis values of shell length,height,width and total weight were 8.5%~24.7%.There existed significant differences in methylated site number and methylated site percentage among the four families(p<0.05).The numbers of total bands of inbred families(A and D) were 729.0 ±19.6 与 717.3 ±28.2 and the methylation levels were(10.0 ±0.9)% and(5.7 ±0.9)%.The numbers of total bands of hybrid families(B and C) were 703.0±10.7 and 726.3±18.8 and the methylation levels were(5.9±0.7)% and(5.7±0.9)%.There existed negative correlation coefficients between mean values of shell length,shell width,shell height and body weight and methylation level,with the coefficients of-0.849,-0.751,-0.674 and-0.652,respectively.
In April of 2012,inbred families(A and D) and hybrid families(B and C) were constructed by two full-sib families of pearl oyster Pinctada fucata martensii.At the age of 2 years,the differences in growth traits of the four families were compared.The methylation levels' differentiation of the four families was evaluated by methylation-sensitive amplification polymorphism(MSAP) technology.The correlation between the methylation ratio and mean values of growth trait were estimated.The results showed that the mean values of shell length,height,width and total weight of hybrid families were larger than inbred families.Heterosis values of shell length,height,width and total weight were 8.5%~24.7%.There existed significant differences in methylated site number and methylated site percentage among the four families(p<0.05).The numbers of total bands of inbred families(A and D) were 729.0 ±19.6 与 717.3 ±28.2 and the methylation levels were(10.0 ±0.9)% and(5.7 ±0.9)%.The numbers of total bands of hybrid families(B and C) were 703.0±10.7 and 726.3±18.8 and the methylation levels were(5.9±0.7)% and(5.7±0.9)%.There existed negative correlation coefficients between mean values of shell length,shell width,shell height and body weight and methylation level,with the coefficients of-0.849,-0.751,-0.674 and-0.652,respectively.
引文
Cao Z.M.,a nd Yang J.,2009,Analysis of the methylation in genome DNA from different tissues of Anodonta woodiana,Shengtai Huanjing Xuebao(Ecology and Environmen),18(6):2011-2016(曹哲明,杨健,2009,背角无齿蚌不同组织的基因组DNA甲基化分析,生态环境学报,18(6):2011-2016)
Deng Y.W.,Liu X.,Wu F.C.,and Zhang G.F.,2008,Experimental evaluation of heterobeltiosis and heterosis between two populations of Pacific abalone Haliotis discus hannai Ino,Acta Oceanologica Sinica,27(4):112-119
Deng Y.W.,L iu X.,Zhang G.F.,and Wu F.C.,2010,Heterosis and combining ability:a diallel cross of three geographically isolated populations of Pacific abalone Haliotis discus hannai Ino,Chinese Journal of Oceanology and Limnology,28(6):1195-1199
Hong Z.,Shi J.S.,Zheng R.H.,Yang L.W.,Chen X.C.,Weng Y.Z.,and Huang J.H.,2009,Epigenetic inheritance and variation of DNA methylation in Chinese Fir(Cunninghamia lanceolata)in traspecific hybrids,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),7(3):591-598(洪舟,施季森,郑仁华,杨立伟,陈孝丑,翁玉榛,黄金华,2009,杉木亲本自交系及其杂交种DNA甲基化和表观遗传变异,分子植物育种,7(3):591-598)
Jiang C.D.,2004,Relationship between DNA methylation and hybrid performances and pig genetic diversity analysis,Dissertation for Ph.D.,Huazhong Agricultural University,Su pervisor:Deng C.Y.,Xiong Y.Z.,pp.34-36(蒋曹德,2004,猪DNA甲基化与杂种表现的关系及遗传多样性分析,博士学位论文,华中农业大学,导师:邓昌彦,熊远著,pp.34-36)
Jiang Q.,Yu H.,Kong L.F.,and Li Q.,2014,Analysis of DNAmethylation in different tissues of the Pacific oyster(Crassostrea gigas)with the fluorescence-labeled methylationsensitive amplified polymorphism(F-MSAP),Zhongguo Shuichan Kexue(Journal of Fishery Sciences of China),21(4):676-683(姜群,于红,孔令锋,李琪,2014,太平洋牡蛎不同组织DNA甲基化的F-MSAP分析,中国水产科学,21(4):676-683)
Li J.H.,Luo Y.Q.,Zhong Y.S.,Huang Y.,Huang R.L.,Wang Q.H.,and Deng Y.W.,2017,Effects of inbreeding on growth traits,genetic diversity and biomineraliazation gene expression of Pinctada fuctada martensii,Nanfang Nongye Xuebao(Journal of Southern Agriculture),48(1):132-138(李俊辉,罗燕秋,钟雅诗,黄怡,黄荣莲,王庆恒,邓岳文,2017,近交对马氏珠母贝生长性状、遗传多样性及矿化基因表达的影响,南方农业学报,48(1):132-138)
Liu X.L.,Chang Y.Q.,Xiang J.H.,Liu X.J.,Li F.H.,and Liu B.Z.,2003,The medium-term growth and development of hybrid between Chinese and Japanese populations of Chlamys farreri,Shuichan Xuebao(Journal of Fisheries of China),27(3):193-199(刘小林,常亚青,相建海,刘宪杰,李富花,刘保忠,2003,栉孔扇贝中国种群与日本种群杂交一代的中期生长发育,水产学报,27(3):193-199)
Luo S.J.,Deng Y.W.,Zheng Z.,Jiao Y.,and Wang Q.H.,2016,Analysis of genomic DNA methylation on different regions of mantle tissue from Pinctada martensii by methylation-sensitive amplification polymorphism,Zhongguo Shuichan Kexue(Journal of Fishery Sciences of China),23(6):1227-1235(罗少杰,邓岳文,郑哲,焦钰,王庆恒,2016,马氏珠母贝外套膜不同区域基因组DNA甲基化MSAP分析,中国水产科学,23(6):1227-1235)
Luo S.J.,Zhang P.F.,Jiao Y.,Wang Q.H.,Du X.D.,and Deng Y.W.,2017,Comparison of DNA methylation polymorphisms between inbred and hybrid families of Pinctada fucata martensii,Guangdong Haiyang Daxue Xuebao(Journal of Guangdong Ocean University),36(6):9-15(罗少杰,张鹏飞,焦钰,王庆恒,杜晓东,邓岳文,2016,马氏珠母贝近交与杂交家系的DNA甲基化多态性比较,广东海洋大学报,36(6):9-15)
Lv J.,Hou R.,Li N.,Wang C.,Zhang L.L.,Hu X.L.,Wang S.,and Bao Z.M.,2013,Application of MSAP technique for investigation of genome-wide DNA methylation level in scallops,Zhongguo Haiyang Daxue Xuebao(Periodical of Ocean University of China),43(10):48-53(吕佳,侯睿,李宁,王宸,张玲玲,胡晓丽,王师,包振民,2013,应用MSAP技术研究扇贝全基因组DNA甲基化水平,中国海洋大学学报,43(10):48-53)
Sun D.X.,Wang D.,Zhang Y.,Yu Y.,Xu G.Y.,and Li J.Y.,2004,Differentially expressed genes between chicken hybrids and their parents and their relationship with heterosis,Nongye Shengwu Jishu Xuebao(Journal of Agricultura l Biotechnology),12(5):548-551(孙东晓,王栋,张沅,俞英,徐桂云,李俊英,2004,杂种鸡与亲本之间差异表达基因及其与杂种优势的关系,农业生物技术学报,12(5):548-551)
Xu C.L.,Sun X.M.,and Zhang S.G.,2013,Mechanism on differential gene expression and heterosis formation,Yichuan(Hereditas),35(6):714-726(许晨璐,孙晓梅,张守攻,2013,基因差异表达与杂种优势形成机制探讨,遗传,35(6):714-726)
Yu T.,Yang A.G.,Wu B.,and Zhou L.Q.,2010,Analysis of Chlamys farreri,Patinopecten yessoensis and their offspring using methylation-sensitive amplification polymorphism(MSAP),Shuichan Xuebao(Journal of Fisheries of China),34(9):1335-1342(于涛,杨爱国,吴彪,周丽青,2010,栉孔扇贝、虾夷扇贝及其杂交子代的MSAP分析,水产学报,34(9):1335-1342)
Wang A.M.,Wang Y.,Gu Z.F.,Li M.,Shi Y.H.,and Li S.F.,2010,Heterosis and genetic variation of hybrids from two geographical populations of pearl oyster,Pinctada martenii,Haiyang Yu Huzhao(Oceanologia et Limnologia Sinica),41(1):140-147(王爱民,王嫣,顾志峰,黎明,石耀华,李思发,2010,马氏珠母贝Pinctada martensii 2个地理群体杂交子代的杂种优势和遗传变异,海洋与湖沼,41(1):140-147)
Wang X.L.,Wang Q.,Shu J.H.,Cai Y.M.,Liu B.,and Wu J.H.,2015,Analysis of nitrogen stress on DNA methylation by MSAP in tall fescue,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(11):2362-2371(王小利,王茜,舒键虹,蔡一鸣,刘彬,吴佳海,2015,氮胁迫下高羊茅基因组DNA甲基化的MSAP分析,基因组学与应用生物学,34(11):2362-2371)
Zhang G.F.,Wang J.H.,Zhao H.E.,Que H.Y.,Liu X.,2002,The RAPD marker of self-bred and hybrid progeny between Chinese and Japanese populations of Haliotis discus hannai Ino,Haiyang Yu Huzao(Oceanologia et Limnologia Sinica),33(5):484-491(张国范,王继红,赵洪恩,阙华勇,刘晓,2002,皱纹盘鲍中国群体和日本群体的自交与杂交F1的RAPD标记,海洋与湖沼,33(5):484-491)
Deng Y.W.,Liu X.,Wu F.C.,and Zhang G.F.,2008,Experimental evaluation of heterobeltiosis and heterosis between two populations of Pacific abalone Haliotis discus hannai Ino,Acta Oceanologica Sinica,27(4):112-119
Deng Y.W.,L iu X.,Zhang G.F.,and Wu F.C.,2010,Heterosis and combining ability:a diallel cross of three geographically isolated populations of Pacific abalone Haliotis discus hannai Ino,Chinese Journal of Oceanology and Limnology,28(6):1195-1199
Hong Z.,Shi J.S.,Zheng R.H.,Yang L.W.,Chen X.C.,Weng Y.Z.,and Huang J.H.,2009,Epigenetic inheritance and variation of DNA methylation in Chinese Fir(Cunninghamia lanceolata)in traspecific hybrids,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),7(3):591-598(洪舟,施季森,郑仁华,杨立伟,陈孝丑,翁玉榛,黄金华,2009,杉木亲本自交系及其杂交种DNA甲基化和表观遗传变异,分子植物育种,7(3):591-598)
Jiang C.D.,2004,Relationship between DNA methylation and hybrid performances and pig genetic diversity analysis,Dissertation for Ph.D.,Huazhong Agricultural University,Su pervisor:Deng C.Y.,Xiong Y.Z.,pp.34-36(蒋曹德,2004,猪DNA甲基化与杂种表现的关系及遗传多样性分析,博士学位论文,华中农业大学,导师:邓昌彦,熊远著,pp.34-36)
Jiang Q.,Yu H.,Kong L.F.,and Li Q.,2014,Analysis of DNAmethylation in different tissues of the Pacific oyster(Crassostrea gigas)with the fluorescence-labeled methylationsensitive amplified polymorphism(F-MSAP),Zhongguo Shuichan Kexue(Journal of Fishery Sciences of China),21(4):676-683(姜群,于红,孔令锋,李琪,2014,太平洋牡蛎不同组织DNA甲基化的F-MSAP分析,中国水产科学,21(4):676-683)
Li J.H.,Luo Y.Q.,Zhong Y.S.,Huang Y.,Huang R.L.,Wang Q.H.,and Deng Y.W.,2017,Effects of inbreeding on growth traits,genetic diversity and biomineraliazation gene expression of Pinctada fuctada martensii,Nanfang Nongye Xuebao(Journal of Southern Agriculture),48(1):132-138(李俊辉,罗燕秋,钟雅诗,黄怡,黄荣莲,王庆恒,邓岳文,2017,近交对马氏珠母贝生长性状、遗传多样性及矿化基因表达的影响,南方农业学报,48(1):132-138)
Liu X.L.,Chang Y.Q.,Xiang J.H.,Liu X.J.,Li F.H.,and Liu B.Z.,2003,The medium-term growth and development of hybrid between Chinese and Japanese populations of Chlamys farreri,Shuichan Xuebao(Journal of Fisheries of China),27(3):193-199(刘小林,常亚青,相建海,刘宪杰,李富花,刘保忠,2003,栉孔扇贝中国种群与日本种群杂交一代的中期生长发育,水产学报,27(3):193-199)
Luo S.J.,Deng Y.W.,Zheng Z.,Jiao Y.,and Wang Q.H.,2016,Analysis of genomic DNA methylation on different regions of mantle tissue from Pinctada martensii by methylation-sensitive amplification polymorphism,Zhongguo Shuichan Kexue(Journal of Fishery Sciences of China),23(6):1227-1235(罗少杰,邓岳文,郑哲,焦钰,王庆恒,2016,马氏珠母贝外套膜不同区域基因组DNA甲基化MSAP分析,中国水产科学,23(6):1227-1235)
Luo S.J.,Zhang P.F.,Jiao Y.,Wang Q.H.,Du X.D.,and Deng Y.W.,2017,Comparison of DNA methylation polymorphisms between inbred and hybrid families of Pinctada fucata martensii,Guangdong Haiyang Daxue Xuebao(Journal of Guangdong Ocean University),36(6):9-15(罗少杰,张鹏飞,焦钰,王庆恒,杜晓东,邓岳文,2016,马氏珠母贝近交与杂交家系的DNA甲基化多态性比较,广东海洋大学报,36(6):9-15)
Lv J.,Hou R.,Li N.,Wang C.,Zhang L.L.,Hu X.L.,Wang S.,and Bao Z.M.,2013,Application of MSAP technique for investigation of genome-wide DNA methylation level in scallops,Zhongguo Haiyang Daxue Xuebao(Periodical of Ocean University of China),43(10):48-53(吕佳,侯睿,李宁,王宸,张玲玲,胡晓丽,王师,包振民,2013,应用MSAP技术研究扇贝全基因组DNA甲基化水平,中国海洋大学学报,43(10):48-53)
Sun D.X.,Wang D.,Zhang Y.,Yu Y.,Xu G.Y.,and Li J.Y.,2004,Differentially expressed genes between chicken hybrids and their parents and their relationship with heterosis,Nongye Shengwu Jishu Xuebao(Journal of Agricultura l Biotechnology),12(5):548-551(孙东晓,王栋,张沅,俞英,徐桂云,李俊英,2004,杂种鸡与亲本之间差异表达基因及其与杂种优势的关系,农业生物技术学报,12(5):548-551)
Xu C.L.,Sun X.M.,and Zhang S.G.,2013,Mechanism on differential gene expression and heterosis formation,Yichuan(Hereditas),35(6):714-726(许晨璐,孙晓梅,张守攻,2013,基因差异表达与杂种优势形成机制探讨,遗传,35(6):714-726)
Yu T.,Yang A.G.,Wu B.,and Zhou L.Q.,2010,Analysis of Chlamys farreri,Patinopecten yessoensis and their offspring using methylation-sensitive amplification polymorphism(MSAP),Shuichan Xuebao(Journal of Fisheries of China),34(9):1335-1342(于涛,杨爱国,吴彪,周丽青,2010,栉孔扇贝、虾夷扇贝及其杂交子代的MSAP分析,水产学报,34(9):1335-1342)
Wang A.M.,Wang Y.,Gu Z.F.,Li M.,Shi Y.H.,and Li S.F.,2010,Heterosis and genetic variation of hybrids from two geographical populations of pearl oyster,Pinctada martenii,Haiyang Yu Huzhao(Oceanologia et Limnologia Sinica),41(1):140-147(王爱民,王嫣,顾志峰,黎明,石耀华,李思发,2010,马氏珠母贝Pinctada martensii 2个地理群体杂交子代的杂种优势和遗传变异,海洋与湖沼,41(1):140-147)
Wang X.L.,Wang Q.,Shu J.H.,Cai Y.M.,Liu B.,and Wu J.H.,2015,Analysis of nitrogen stress on DNA methylation by MSAP in tall fescue,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(11):2362-2371(王小利,王茜,舒键虹,蔡一鸣,刘彬,吴佳海,2015,氮胁迫下高羊茅基因组DNA甲基化的MSAP分析,基因组学与应用生物学,34(11):2362-2371)
Zhang G.F.,Wang J.H.,Zhao H.E.,Que H.Y.,Liu X.,2002,The RAPD marker of self-bred and hybrid progeny between Chinese and Japanese populations of Haliotis discus hannai Ino,Haiyang Yu Huzao(Oceanologia et Limnologia Sinica),33(5):484-491(张国范,王继红,赵洪恩,阙华勇,刘晓,2002,皱纹盘鲍中国群体和日本群体的自交与杂交F1的RAPD标记,海洋与湖沼,33(5):484-491)