基于灰色关联分析的茜草醇提物抗氧化、抗肝癌谱效关系研究
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摘要
目的研究茜草特征图谱与其抗氧化、抗肝癌的相关性,探讨茜草抗氧化及抗肝癌活性的物质基础。方法采用高效液相色谱法建立不同批次茜草的指纹图谱;采用ABTS法评价茜草提取物的抗氧化活性,MTT法检测茜草提取物对肝肿瘤细胞的影响;采用灰色关联度分析方法进行"谱-效"关联分析。结果建立茜草特征指纹图谱,标定了26个色谱峰;药效实验结果表明不同茜草样品抗氧化及抑制肝肿瘤细胞(HepG2)增殖活性存在差异;灰色关联分析结果表明7个色谱峰与抗氧化活性密切相关(γ>0.90),分别是1、5、6、9、23、24、26号色谱峰;9个色谱峰与抗肿瘤细胞活性密切相关(γ> 0.90),分别是1、3、6、14、15、18、19、23、24。通过与对照品比对,14、18、19、26号峰为新橙皮苷、山柰酚、茜草素及大叶茜草素。结论茜草抗氧化、抗肿瘤的药效是多种成分共同作用的结果,明确了茜草抗氧化活性与抗肝癌活性的物质基础,为开展以药效为导向的茜草药材质量评价研究提供科学依据。
Objective To determine the correlation between the HPLC of Rubia cordifolia L. and anti-oxidation and anti-hepatocarcinoma of Rubia cordifolia L., and to provide a basis for clarifying the material basis of antiinflammatory and anti-hepatocarcinoma effects. Methods Fingerprints of Rubia cordifolia L. from different habitats were established by HPLC and the antioxidant activity was evaluated by the ABTS method, MTT was used to detect the inhibition rate on the liver tumor cells. Spectrum-effect correlation was determine by grey correlation analysis. Results The fingerprints of Rubia cordifolia L. were established with 26 calibrated peaks.The pharmacodynamics experiments showed different antioxidant activities and inhibition of HepG2 proliferation among the samples of Rubia cordifolia L.. The gray correlation analysis indicated that 7 peaks(No. 1, 5, 6, 9, 23,24 and 26) were closely related to the antioxidant activity(γ > 0.90), 9 peaks(No. 1, 3, 6, 14, 15, 18, 19, 23 and24) were closely related to the anti-tumor cell activity(γ > 0.90), and 4 chemical components(No. 14, 18, 19, 26)were identified: new hesperidin, kaempferol, alizarin and rabimaillin. Conclusion The anti-oxidation and antitumor effect of Rubia cordifolia L. may be the results of a combination of components. The material basis has been identified, which can lay a foundation for the quality control of Rubia cordifolia L. guided by pharmacodynamics.
Objective To determine the correlation between the HPLC of Rubia cordifolia L. and anti-oxidation and anti-hepatocarcinoma of Rubia cordifolia L., and to provide a basis for clarifying the material basis of antiinflammatory and anti-hepatocarcinoma effects. Methods Fingerprints of Rubia cordifolia L. from different habitats were established by HPLC and the antioxidant activity was evaluated by the ABTS method, MTT was used to detect the inhibition rate on the liver tumor cells. Spectrum-effect correlation was determine by grey correlation analysis. Results The fingerprints of Rubia cordifolia L. were established with 26 calibrated peaks.The pharmacodynamics experiments showed different antioxidant activities and inhibition of HepG2 proliferation among the samples of Rubia cordifolia L.. The gray correlation analysis indicated that 7 peaks(No. 1, 5, 6, 9, 23,24 and 26) were closely related to the antioxidant activity(γ > 0.90), 9 peaks(No. 1, 3, 6, 14, 15, 18, 19, 23 and24) were closely related to the anti-tumor cell activity(γ > 0.90), and 4 chemical components(No. 14, 18, 19, 26)were identified: new hesperidin, kaempferol, alizarin and rabimaillin. Conclusion The anti-oxidation and antitumor effect of Rubia cordifolia L. may be the results of a combination of components. The material basis has been identified, which can lay a foundation for the quality control of Rubia cordifolia L. guided by pharmacodynamics.
引文
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[24]倪翀,郭世昌,杜琰,等.饮食营养与炎症-癌症危险性的关系[J].第二军医大学学报,2015,36(10):1117-1122.
[25]郭海洁.氧自由基与妇科肿瘤关系的研究[J].北华大学学报(自然科学版),2008,9(6):518-521.
[2]王建壮,施玉旋,郑荣杰,等.茜草中总黄酮的超声提取工艺研究[J].中国现代中药,2013,15(11):984-986.
[3]张高娃,陈彩霞.茜草总黄酮的提取及其抗氧化作用的研究[J].内蒙古民族大学学报(蒙医药学版),2010,(1):29-32.
[3]田春莲,蒋凤开.茜草总黄酮提取工艺研究[J].食品科学,2011,32(24):60-63.
[4]李俊峰,舒建昌,杨钦河,等.大叶茜草素抑制大鼠CFSC-2G细胞活化和胶原合成[J].中国病理生理杂志,2017,33(12):2259-2263.
[5]Zengin G,Degirmenci NS,Alpsoy L. Evaluation of antioxidant,enzyme inhibition,and cytotoxic activity of three anthraquinones(alizarin,purpurin,and quinizarin)[J].Hum Exp Toxicol,2016,35(5):544-553.
[6]Nam W,Kim SP,Nam SH,et al. Structure-antioxidative and anti-inflammatory activity relationships of purpurin and related anthraquinones in chemical and cell assays[J]. Molecules,2017,22(2):265-279.
[7]Gong XP,Sun YY,Chen W,et al. Anti-diarrheal and anti-inflammatory activities of aqueous extract of the aerial part of Rubia cordifolia[J]. BMC Complement Altern Med,2017,17(1):20-29.
[8]陈毅,王海丽,薛露,等.茜草的研究进展[J].中草药,2017,48(13):2771-2779.
[9]杨红红,符悦冠,肖烽,等.中药茜草根乙酸乙酯萃取物的分离及抗氧化活性研究[J].武汉工程大学学报,2010,32(12):1-5.
[10]王金梅,姚辰,李昌勤,等.基于DPPH、ABTS和FRAP法的红花抗氧化谱效关系研究[J].中国药学杂志,2017,52(10):825-831.
[11]孙广利,许旭东,杨峻山,等.华钩藤中黄酮类化学成分的分离和结构鉴定[J].中国药学杂志,2012,47(3):177-179.
[12]张茂婷.茜草地上部分的化学成分和质量研究[D].合肥:安徽中医药大学,2017.
[13]王佳佳.河南产茜草化学成分与生物活性研究[D].郑州:河南大学,2014.
[14]李鹏,胡正海.茜草的生物学及化学成分与生物活性研究进展[J].中草药,2013,44(14):2009-2014.
[15]赵峰,王素娟,吴秀丽,等.红大戟中的蒽醌类化学成分[J].中国中药杂志,2011,36(21):2980-2986.
[16]旷丽莎,江炜,侯爱君,等.水线草的化学成分研究[J].中草药,2009,40(7):1020-1024.
[17]杨亚滨,杨雪琼,丁中涛.白花蛇舌草化学成分的研究[J].云南大学学报(自然科学版),2007,29(2):187-189,212.
[18]王宏虹,刘华钢,黄慧学,等.两面针提取物抗肝癌谱-效关系研究[J].中草药,2014,45(8):1102-1109.
[19]李慧,孟宪生.中药茜草的本草考证[J/OL].中药材,2018,41(10):2212-2216.
[20]李海峰,肖凌云,张菊,等.茜草化学成分及其药理作用研究进展[J].中药材,20,39(6):1433-1436.
[21]Shen CH,Liu CT,Song XJ,et al. Evaluation of analgesic and anti-inflammatory activities of Rubia cordifolia L.by spectrum-effect relationships[J]. J Chromatogr B Analyt Technol Biomed Life Sci,2018,1090(15):73-80.
[22]ChandrashekarBS,PrabhakaraS,Mohan T,etal.Characterization of Rubia cordifolia L root extract and its evaluation of cardioprotective effect in wistar rat model[J].Indian J Pharmacol,2018,50(1):12-21.
[23]Wang Z,Li MY,Mi C,et al. Mollugin Has an anti-cancer therapeutic effect by inhibiting TNF-α-induced NF-κB activation[J]. Int J Mol Sci,2017,18(8):1619-1632.
[24]倪翀,郭世昌,杜琰,等.饮食营养与炎症-癌症危险性的关系[J].第二军医大学学报,2015,36(10):1117-1122.
[25]郭海洁.氧自由基与妇科肿瘤关系的研究[J].北华大学学报(自然科学版),2008,9(6):518-521.