溶剂解吸-气相色谱法同时测定工作场所空气中1-溴丙烷和2-溴丙烷
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摘要
目的建立工作场所空气中1-溴丙烷(1-BP)和2-溴丙烷(2-BP)的溶剂解吸-气相色谱同时测定方法。方法采用活性炭管采集空气样品,以二硫化碳溶液解吸,DB-FFAP毛细管色谱柱分离,火焰离子化检测器测定。结果 1-BP和2-BP分别在37.24~2 979.00和16.38~1 310.00 mg/L范围内线性关系良好,相关系数均大于0.999 99。4℃冰箱中放置过夜时,1-BP和2-BP平均解吸效率分别为95.30%~96.14%、99.79%~100.76%,批内精密度分别为0.28%~0.68%和0.24%~0.38%,批间精密度分别为0.97%~1.68%和1.28%~1.61%。100 mg活性炭管的1-BP和2-BP平均穿透容量分别大于2.9、2.5 mg。1-BP和2-BP样品在室温下分别至少可保存1和16 d,在4℃冰箱中分别至少可保存10和45 d。结论本方法适用于工作场所空气中1-BP和2-BP的同时测定。
Objective To establish a solvent desorption-gas chromatography method for simultaneous determination of1-bromopropane( 1-BP) and 2-bromopropane( 2-BP) in the workplace air. Methods Samples containing 1-BP and 2-BP in the air were collected with charcoal tubes and desorbed with carbon disulfide solution,then separated with DB-FFAP capillary column and detected with flame ionization detector. Results The linear ranges were 37. 24-2 979. 00 mg / L[correlation coefficient( r) > 0. 999 99] for 1-BP and 16. 38-1 310. 00 mg/L( r > 0. 999 99) for 2-BP. The average desorption efficiencies( at 4 ℃ overnight) were 95. 30%-96. 14% and 99. 79%-100. 76%,the intraassay precisions were0. 28%-0. 68% and 0. 24%-0. 38%,the intraassay precisions were 0. 97%-1. 68% and 1. 28%-1. 61% for 1-BP and2-BP,respectively. The average penetration capacity of 1-BP and 2-BP of 100 mg activated carbon tube were greater than2. 9,2. 5 mg,respectively. The samples of 1-BP and 2-BP could be stored for 1 and 16 days at room temperature,and 10 and 45 days in refrigerator at the temperature of 4 ℃,respectively. Conclusion This method was appropriately applied for simultaneous determination of 1-BP and 2-BP in the workplace air.
Objective To establish a solvent desorption-gas chromatography method for simultaneous determination of1-bromopropane( 1-BP) and 2-bromopropane( 2-BP) in the workplace air. Methods Samples containing 1-BP and 2-BP in the air were collected with charcoal tubes and desorbed with carbon disulfide solution,then separated with DB-FFAP capillary column and detected with flame ionization detector. Results The linear ranges were 37. 24-2 979. 00 mg / L[correlation coefficient( r) > 0. 999 99] for 1-BP and 16. 38-1 310. 00 mg/L( r > 0. 999 99) for 2-BP. The average desorption efficiencies( at 4 ℃ overnight) were 95. 30%-96. 14% and 99. 79%-100. 76%,the intraassay precisions were0. 28%-0. 68% and 0. 24%-0. 38%,the intraassay precisions were 0. 97%-1. 68% and 1. 28%-1. 61% for 1-BP and2-BP,respectively. The average penetration capacity of 1-BP and 2-BP of 100 mg activated carbon tube were greater than2. 9,2. 5 mg,respectively. The samples of 1-BP and 2-BP could be stored for 1 and 16 days at room temperature,and 10 and 45 days in refrigerator at the temperature of 4 ℃,respectively. Conclusion This method was appropriately applied for simultaneous determination of 1-BP and 2-BP in the workplace air.
引文
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[10]李宏玲,宋向荣,蒋柳权,等.1-溴丙烷吸入对大鼠肝和血液毒性研究[J].中国职业医学,2014,41(4):367-373.
[11]罗巧,宋向荣,李宏玲,等.1-溴丙烷吸入染毒对Wistar大鼠睾丸毒性研究[J].中国职业医学,2015,42(3):268-272.
[12]National Toxicology Program.Report on carcinogens monograph on1-bromopropane[J].Rep Carcinog Monogr,2013,(13-5982):1-168.
[13]Easley CA 4th,Bradner JM,Moser A,et al.Assessing reproductive toxicity of two environmental toxicants with a novel in vitro human spermatogenic model[J].Stem Cell Res,2015,14(3):347-355.
[14]Park J,Shin KS,Kim Y.Occupational reproductive function abnormalities and bladder cancer in Korea[J].J Korean Med Sci,2010,25(Suppl):S41-S45.
[2]Chalupka S.Reducing workplace exposure to 1-bromopropane[J].Workplace Health Saf,2014,62(3):128.
[3]National Institute for Occupational Safety and Health.Registry of toxic effects of chemical substances:RTECS#:TX4110000[EB/OL].(2009-02)[2015-06-25].http://www.cdc.gov/nioshrtecs/tx3eb6b0.html.
[4]Occupational Safety and Health Adiministration.Sampling and analytical methods:1-bromopropane,method PV2061[EB/OL].(1999-03-10)[2015-06-23].http://www.osha.gov/dts/sltc/methods/partial/pv2061/2061.html.
[5]Occupational Safety and Health Adiministration.Sampling and analytical methods:2-bromopropane,method PV2062[EB/OL].(1999-03-10)[2015-06-23].http://www.osha.gov/dts/sltc/methods/partial/pv2062/2062.html.
[6]中华人民共和国卫生部.GBZ/T 210.4—2008职业卫生标准制定指南第4部分:工作场所空气中化学物质测定方法[S].北京:人民卫生出版社,2008.
[7]中华人民共和国卫生部.GBZ 159—2004工作场所空气中有害物质监测的采样规范[S].北京:人民卫生出版社,2006.
[8]National Institute for Occupational Safety and Health.NIOSH manual of analytical methods(NMAM):1-and 2-bromopropane,method1025[EB/OL].(2003-03-15)[2015-06-23].http://www.cdc.gov/niosh/docs/2003-154/pdfs/1025.pdf.
[9]Huang Z,Ichihara S,Oikawa S,et al.Hippocampal phosphoproteomics of F344 rats exposed to 1-bromopropane[J].Toxicol Appl Pharmacol,2015,282(2):151-160.
[10]李宏玲,宋向荣,蒋柳权,等.1-溴丙烷吸入对大鼠肝和血液毒性研究[J].中国职业医学,2014,41(4):367-373.
[11]罗巧,宋向荣,李宏玲,等.1-溴丙烷吸入染毒对Wistar大鼠睾丸毒性研究[J].中国职业医学,2015,42(3):268-272.
[12]National Toxicology Program.Report on carcinogens monograph on1-bromopropane[J].Rep Carcinog Monogr,2013,(13-5982):1-168.
[13]Easley CA 4th,Bradner JM,Moser A,et al.Assessing reproductive toxicity of two environmental toxicants with a novel in vitro human spermatogenic model[J].Stem Cell Res,2015,14(3):347-355.
[14]Park J,Shin KS,Kim Y.Occupational reproductive function abnormalities and bladder cancer in Korea[J].J Korean Med Sci,2010,25(Suppl):S41-S45.