重组鸡Ex-FABP蛋白对福美双诱导的TD肉鸡血清中Bcl-2含量的影响
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摘要
为研究细胞外脂肪酸结合蛋白Ex-FABP对胫骨软骨发育不良(TD)肉鸡血清中凋亡因子B细胞淋巴瘤因子2(Bcl-2)的影响,试验采用连续2 d饲喂含有福美双的日粮来诱导构建肉鸡TD模型,共设置4组正常日粮组(健康组)、4组添加福美双日粮组(福美双组)和1组空白对照组,然后注射Ex-FABP蛋白。结果表明,福美双组和健康组分别注射重组Ex-FABP蛋白后,血清中Bcl-2蛋白浓度相比福美双对照组、健康对照组和空白对照组显著升高。重组鸡Ex-FABP蛋白可能通过刺激机体血清中Bcl-2蛋白的表达,从而抑制软骨生长板细胞凋亡,以发挥治疗TD的作用。研究结果可为TD肉鸡的治疗提供新的思路和方法。
To study the effect of extracellular fatty acid binding protein(Ex-FABP)on the expression of apoptotic factor B-cell lymphoma gene 2(Bcl-2)in tibial dyschondroplasia(TD)broilers, in this experiment, a diet containing thiram was fed for two days to induce the TD model. 4 normal diet groups(healthy groups), 4 thiram-added diet groups(thiram groups)and one control group were set up in this study. The healthy groups and thiram groups were injected with Ex-FABP protein. The results showed that the concentrations of Bcl-2 protein in the thiram-fed group and the healthy group were significantly higher than that in the thiram-fed control group, healthy control group and blank control group after injection of recombinant Ex-FABP protein. The test indicated that the recombinant chicken Ex-FABP protein may inhibit the apoptosis of chondrocyte growth plate cells by stimulating the expression of Bcl-2 protein in the serum,and it may participate in the treatment of TD. The results will provide a new idea for the treatment of TD.
To study the effect of extracellular fatty acid binding protein(Ex-FABP)on the expression of apoptotic factor B-cell lymphoma gene 2(Bcl-2)in tibial dyschondroplasia(TD)broilers, in this experiment, a diet containing thiram was fed for two days to induce the TD model. 4 normal diet groups(healthy groups), 4 thiram-added diet groups(thiram groups)and one control group were set up in this study. The healthy groups and thiram groups were injected with Ex-FABP protein. The results showed that the concentrations of Bcl-2 protein in the thiram-fed group and the healthy group were significantly higher than that in the thiram-fed control group, healthy control group and blank control group after injection of recombinant Ex-FABP protein. The test indicated that the recombinant chicken Ex-FABP protein may inhibit the apoptosis of chondrocyte growth plate cells by stimulating the expression of Bcl-2 protein in the serum,and it may participate in the treatment of TD. The results will provide a new idea for the treatment of TD.
引文
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[2]张宁,李欣,宁官保,等.福美双诱导的肉鸡胫骨软骨发育不良软骨细胞凋亡[J].畜牧兽医学报,2017(12):2421-2428.
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[9] RATH N C,HUFF W E,HUFF G R. Thiram-induced changes in the expression of genes relating to vascularization and tibial dyschondroplasia[J]. Poultry Science,2007,86(11):2390-2395.
[10]吴国瑞.低氧微环境对脑星形胶质细胞凋亡及对BCL-2表达的影响[D].昆明:昆明医科大学,2015.
[11] DESCALZI C F,MANDUCA P,TACCHETTI C,et al. Developmentally regulated synthesis of a low molecular weight protein(Ch21)by differentiating chondrocytes[J]. Journal of Cell Biology,1988,107(6):2455-2463.
[12]BEDARD P A,YANNONI Y,SIMMONS D L,et al. Rapid repression of quiescence-specific gene expression by epidermal growth factor,insulin,and pp60v-src[J]. Molecular and Cellular Biology,1989,9(3):1371-1375.
[13] CORRENTI C,CLIFTON M C,ABERGEL R J,et al. Galline Ex-FABP is an antibacterial siderocalin and a lysophosphatidic acid sensor functioning through dual ligand specificities[J]. Structure,2011,19(12):1796-1806.
[14] CANCEDDA F D,DOZIN B,ROSSI F,et al. The Ch21 protein,developmentally regulated in chick embryo,belongs to the superfamily of lipophilic molecule carrier proteins[J]. Journal of Biological Chemistry,1990,265(31):19060-19064.
[15] TIAN W X,LI J K,QIN P,et al. Screening of differentially expressed genes in the growth plate of broiler chickens with tibial dyschondroplasia by microarray analysis[J]. BMC Genomics,2013,14:276.
[16] CERMELLI S,ZEREGA B,CARLEVARO M,et al. Extracellular fatty acid binding protein(Ex-FABP)modulation by inflammatory agents:"physiological"acute phase response in endochondral bone formation[J]. European Journal of Cell Biology,2000,79(3):155-164.
[17]张宁,李桢,李娜娜,等.重组鸡GSTA3蛋白对福美双诱导的TD肉鸡血清中Bax含量的影响[J].山西农业科学,2017,45(11):1849-1852.
[18] WANG C X,NIU S,JAHEJO A R,et al. Identification of apoptosis-related genes in erythrocytes of broiler chickens and their response to thiram-induced tibial dyschondroplasia and recombinant glutathione-S-transferase A3 protein[J]. Research in Veterinary Science,2018,120:11-16.
[2]张宁,李欣,宁官保,等.福美双诱导的肉鸡胫骨软骨发育不良软骨细胞凋亡[J].畜牧兽医学报,2017(12):2421-2428.
[3]田文霞.肉鸡胫骨软骨发育不良的研究[M].北京:中国农业科学技术出版社,2011.
[4]孙卫东,王小龙.饮水高氯诱发肉鸡胫骨软骨发育不良的试验研究[J].畜牧兽医学报,2000,31(4):331-336.
[5] RIDDELL C. The development of tibial dyschondroplasia in broiler chickens[J]. Avian Diseases,1975,19(3):443.
[6]田文霞,李家奎,毕丁仁,等.福美双对肉鸡生长性能和胫骨软骨发育不良组织病理学变化的影响[J].畜牧兽医学报,2008,39(6):733-738.
[7]田文霞.福美双诱发肉鸡胫骨软骨发育不良差异表达基因的研究[D].武汉:华中农业大学,2008.
[8] RATH N C,HUFF W E,BALOG J M,et al. Comparative efficacy of different dithiocarbamates to induce tibial dyschondroplasia in poultry[J]. Poultry Science,2004,83(2):266-274.
[9] RATH N C,HUFF W E,HUFF G R. Thiram-induced changes in the expression of genes relating to vascularization and tibial dyschondroplasia[J]. Poultry Science,2007,86(11):2390-2395.
[10]吴国瑞.低氧微环境对脑星形胶质细胞凋亡及对BCL-2表达的影响[D].昆明:昆明医科大学,2015.
[11] DESCALZI C F,MANDUCA P,TACCHETTI C,et al. Developmentally regulated synthesis of a low molecular weight protein(Ch21)by differentiating chondrocytes[J]. Journal of Cell Biology,1988,107(6):2455-2463.
[12]BEDARD P A,YANNONI Y,SIMMONS D L,et al. Rapid repression of quiescence-specific gene expression by epidermal growth factor,insulin,and pp60v-src[J]. Molecular and Cellular Biology,1989,9(3):1371-1375.
[13] CORRENTI C,CLIFTON M C,ABERGEL R J,et al. Galline Ex-FABP is an antibacterial siderocalin and a lysophosphatidic acid sensor functioning through dual ligand specificities[J]. Structure,2011,19(12):1796-1806.
[14] CANCEDDA F D,DOZIN B,ROSSI F,et al. The Ch21 protein,developmentally regulated in chick embryo,belongs to the superfamily of lipophilic molecule carrier proteins[J]. Journal of Biological Chemistry,1990,265(31):19060-19064.
[15] TIAN W X,LI J K,QIN P,et al. Screening of differentially expressed genes in the growth plate of broiler chickens with tibial dyschondroplasia by microarray analysis[J]. BMC Genomics,2013,14:276.
[16] CERMELLI S,ZEREGA B,CARLEVARO M,et al. Extracellular fatty acid binding protein(Ex-FABP)modulation by inflammatory agents:"physiological"acute phase response in endochondral bone formation[J]. European Journal of Cell Biology,2000,79(3):155-164.
[17]张宁,李桢,李娜娜,等.重组鸡GSTA3蛋白对福美双诱导的TD肉鸡血清中Bax含量的影响[J].山西农业科学,2017,45(11):1849-1852.
[18] WANG C X,NIU S,JAHEJO A R,et al. Identification of apoptosis-related genes in erythrocytes of broiler chickens and their response to thiram-induced tibial dyschondroplasia and recombinant glutathione-S-transferase A3 protein[J]. Research in Veterinary Science,2018,120:11-16.