DLX1基因在人牙齿发育中的表达
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摘要
目的:研究DLX1基因在人牙齿发育中的表达模式,确定DLX1转录子亚型。方法:提取人牙胚总RNA,利用巢式RT-PCR技术克隆DLX1cDNA序列;制备地高辛标记的RNA探针,检测人磨牙发育不同时期DLX1的表达情况。结果:人牙胚中表达的DLX1为转录子亚型1。在表达模式上,11周、14周、17周和19周4个不同发育时期磨牙均有DLX1表达,11周和14周少量表达于牙上皮和间充质,17周依然在牙上皮和间充质中表达,但表达增强,19周表达主要集中在内釉上皮细胞。结论:DLX1在人牙齿发育的各个时期均有表达,表明DLX1可能是人类牙齿发育中的一个重要调节因子。
Objective:To examine the expression pattern and determine the transcript variant of DLX1 gene in the human embryonic tooth germ.Methods:DLX1cDNA fragment was cloned from the human developing tooth germ by nested RT-PCR.The expression patterns of DLX1 gene at multiple molar developing stages(11W,14W,17W and 19W)were examined using biotin-labelled in situ hybridization.Results:DLX1 gene expressed at human tooth germ is transcript variant 1.Weak expression of DLX1was detected in the dental epithelium and the dental mesenchyme at 11W and 14W,which increased at 17W.At 19W,expression of DLX1 was only restricted in the inner enamel epithelium.Conclusion:Expression of DLX1 was detected in multiple developing stages of molar,indicating that DLX1 may be a critical regulation factor in human tooth development.
Objective:To examine the expression pattern and determine the transcript variant of DLX1 gene in the human embryonic tooth germ.Methods:DLX1cDNA fragment was cloned from the human developing tooth germ by nested RT-PCR.The expression patterns of DLX1 gene at multiple molar developing stages(11W,14W,17W and 19W)were examined using biotin-labelled in situ hybridization.Results:DLX1 gene expressed at human tooth germ is transcript variant 1.Weak expression of DLX1was detected in the dental epithelium and the dental mesenchyme at 11W and 14W,which increased at 17W.At 19W,expression of DLX1 was only restricted in the inner enamel epithelium.Conclusion:Expression of DLX1 was detected in multiple developing stages of molar,indicating that DLX1 may be a critical regulation factor in human tooth development.
引文
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[2]Zerucha T,and M Ekker.Distal-less-related homeobox genes of vertebrates:evolution,function,and regulation[J].Biochem Cell Biol,2000,78(5)∶593-601
[3]Lezot F,Thomas B,Greene SR,et al.Physiological implications of DLX homeoproteins in enamel formation[J].J Cell Physiol,2008,216(3)∶688-97
[4]Qiu M,Bulfone A,Ghattas I,et al.Role of the Dlx homeobox genes in proximodistal patterning of the branchial arches:mutations of Dlx-1,Dlx-2,and Dlx-1and-2alter morphogenesis of proximal skeletal and soft tissue structures derived from the first and second arches[J].Dev Biol,1997,185(2)∶165-84
[5]Park BK,Sperber SM,Choudhury A,et al.Intergenic enhancers with distinct activities regulate Dlx gene expression in the mesenchyme of the branchial arches[J].Developmental biology,2004,268(2)∶532-545
[6]McKinsey GL,Lindtner S,Trzcinski B,et al.Dlx1&2-dependent expression of Zfhx1b(Sip1,Zeb2)regulates the fate switch between cortical and striatal interneurons[J].Neuron,2013,77(1)∶83-98
[7]Ghosh AK,Shankar DB,Shackleford GM,et al.Molecular cloning and characterization of human FGF8alternative messenger RNA forms[J].Cell Growth Differ,1996,7(10)∶1425-34
[8]Zhang Y,Zhao X,Hu Y,et al.Msx1is required for the induction of Patched by Sonic hedgehog in the mammalian tooth germ[J].Dev Dyn,1999,215(1)∶45-53
[9]Cohen SM,G Jürgens,Proximal-distal pattern formation in Drosophila:cell autonomous requirement for Distal-less gene activity in limb development[J].The EMBO journal,1989,8(7)∶2045
[10]孙昊,姜文辉,王旭东.Dlx1/Dlx2基因对小鼠第一鳃弓发育调控机制的研究进展[J].中华口腔医学研究杂志(电子版),2010,4(1)∶56-59
[11]Hamilton SP,Woo JM,Carlson EJ,et al.Analysis of four DLX homeobox genes in autistic probands[J].BMC Genet,2005,6∶52