杭白菊中多酚物质的提取及其抗氧化活性
|
摘要
以杭白菊为原料,以多酚得率为评价指标,在十二烷基磺酸钠(SDS)体积分数、液固比[每克(g)原料所用SDS溶液的体积(m L)]、超声时间、超声温度、超声功率单因素实验基础上,采用响应面法优化了杭白菊中多酚的提取工艺,并对杭白菊多酚组分进行了分析。测定了杭白菊多酚的总还原力以及对1,1-二苯基-2-苦基肼自由基(DPPH·)、HO·的清除能力。结果表明,SDS辅助超声波法提取杭白菊多酚的最佳工艺条件为:SDS的体积分数为1.75%、液固比为35 m L/g、超声时间为30 min、超声温度为65℃。在此条件下,多酚得率为78.023 mg/g,预测值为78.248 mg/g。杭白菊多酚主要成分为异绿原酸A,含量为40.87 mg/g,其次为绿原酸21.34 mg/g、木犀草苷8.65 mg/g。杭白菊多酚的总还原力、清除HO·的半抑制浓度(IC50)分别为0.0037、1.4650g/L,强于维生素C(VC)的0.1611、2.1850 g/L和2,6-二叔丁基-4-甲基苯酚(BHT)的2.0620、1.6360 g/L;杭白菊多酚清除DPPH·的IC50为0.0456 g/L,弱于VC强于BHT。
Response surface methodology was used to optimize the extraction process of polyphenols from Chrysanthemum morifolium Ramat.. The parameters such as volume fraction of SDS(sodium laurylsulfonate),liquid-solid ratio(the volume of SDS solution used per gram of raw material,the same below),ultrasonic time and ultrasonic temperature were optimized on the basis of extraction rate of polyphenols,and the composition of polyphenols were analyzed. Moreover,the antioxidant activity of polyphenols was measured using the total reducing power,DPPH·(l,1-diphenyl-2-picryhydrazyl radical) and HO·(hydroxyl radical) scavenging. The results showed that the optimal extraction conditions for polyphenols by SDS assisted ultrasonic method were as follows: volume fraction of SDS 1.75%,liquid-solid ratio 35 m L/g,ultrasonic time 30 min,and ultrasonic temperature 65℃. Under these conditions,an extraction rate of 78.023 mg/g for polyphenol from Chrysanthemum morifolium Ramat. was obtained,compared with the predictive value of 78.248 mg/g. The results of chromatography analysis with HPLC show that isochlorogenicacid A,chlorogenic acid,galuteolin,are the main components existing in polyphenols.The content of 3 respective components were 40.87,21.34 and 8.65 mg/g. The IC50 values of the total reducing power,scavenging OH radical were 0.0037,1.4650 g/L,stronger than that of VC(Vitamin C)(0.1611,2.1850 g/L) and BHT(butylate hydroxytoluene)(2.0620、1.6360 g/L).The scavenging DPPH radical(IC50 was 0.0456 g/L) was weaker than that of VC,but stronger than that of BHT. The extraction process was stable and reasonable,accurate and reliable,and it can be used to extract the polyphenol from Chrysanthemum morifolium Ramat..The result will provide basis for the further development andutilization of polyphenols of Chrysanthemum morifolium Ramat..
Response surface methodology was used to optimize the extraction process of polyphenols from Chrysanthemum morifolium Ramat.. The parameters such as volume fraction of SDS(sodium laurylsulfonate),liquid-solid ratio(the volume of SDS solution used per gram of raw material,the same below),ultrasonic time and ultrasonic temperature were optimized on the basis of extraction rate of polyphenols,and the composition of polyphenols were analyzed. Moreover,the antioxidant activity of polyphenols was measured using the total reducing power,DPPH·(l,1-diphenyl-2-picryhydrazyl radical) and HO·(hydroxyl radical) scavenging. The results showed that the optimal extraction conditions for polyphenols by SDS assisted ultrasonic method were as follows: volume fraction of SDS 1.75%,liquid-solid ratio 35 m L/g,ultrasonic time 30 min,and ultrasonic temperature 65℃. Under these conditions,an extraction rate of 78.023 mg/g for polyphenol from Chrysanthemum morifolium Ramat. was obtained,compared with the predictive value of 78.248 mg/g. The results of chromatography analysis with HPLC show that isochlorogenicacid A,chlorogenic acid,galuteolin,are the main components existing in polyphenols.The content of 3 respective components were 40.87,21.34 and 8.65 mg/g. The IC50 values of the total reducing power,scavenging OH radical were 0.0037,1.4650 g/L,stronger than that of VC(Vitamin C)(0.1611,2.1850 g/L) and BHT(butylate hydroxytoluene)(2.0620、1.6360 g/L).The scavenging DPPH radical(IC50 was 0.0456 g/L) was weaker than that of VC,but stronger than that of BHT. The extraction process was stable and reasonable,accurate and reliable,and it can be used to extract the polyphenol from Chrysanthemum morifolium Ramat..The result will provide basis for the further development andutilization of polyphenols of Chrysanthemum morifolium Ramat..
引文
[1]Zhong Aijiao(钟爱娇),Jiang Zhe(姜哲),Li Xuezheng(李雪征),et al.Research progress on chemical constituent and pharmacological activities of Chrysanthemi Flos[J].Drugs&Clinic(现代药物与临床),2014,29(7):824-830.
[2]Parzonko A,Naruszewicz M.Cardioprotective effects of Aronia melanocarpa anthocynanins.from laboratory experiments to clinical practice[J].Current Pharmaceutical Design,2016,22(2):174-179.
[3]Kang N J,Shin S H,Lee H J,et al.Polyphenols as small molecular inhibitors of signaling cascades in carcinogenesis[J].Pharmacology&Therapeutics,2011,130(3):310-324.
[4]Tang Hehua(汤鹤华).Extraction of the total polyphenolic compounds from Florist Chrysanthemum on by ultrasonic aid[J].Jiangxi Chemical Industry(江西化工),2007,(4):110-112.
[5]Gong Jinyan(龚金炎),Ge Qing(葛青),Cheng Lichun(陈丽春)et al.Optimization extraction of polyphenols from Hangzhou white Chrysanthemum[J].Food Science and Technology(食品科技),2013,38(10):224-227.
[6]Kuang Chunlin(邝春林).Antioxidant,antibacterial activity of component extracted from Chrysanthemum morifolium Ramat.and Coreopsis tinctoria Nuff.[D]Ya'an:Sichuan Agricultural University(四川农业大学),2016.
[7]Li Yu(黎彧).Studies of the surfactan assisted microwave extraction of the pigment from Begonia fimbristipula Hance[J].Journal of Instrumental Analysis(分析测试学报),2005,24(4):95-97.
[8]Wang Yongsheng(王永胜).Determination and analysis of active substances in surfactant[J].Popular Standardzation(大众标准化),2013,(2):60-61.
[9]Chemat F,Huma Z,Khan M K.Applications of ultrasound in food technology:Processing,preservation and extraction[J].Ultrasonics Sonochemistry,2011,18(4):813-835.
[10]Wang Youqiong(王有琼),Ma Liyi(马李一),Zhang Chongquan(张重权),et al.Antioxidant capacity and extraction technique of polyphenol from the leaf of Azadirachta indica[J].Fine Chemicals(精细化工),2015,32(1):64-68.
[11]Zu Yuangang(祖元刚),Hu Yan(胡艳),Jiang Shougang(姜守刚).Extraction and anti-inflammatory of Korean pine polyphenol[J].Bulletin of Botanical Research(植物研究),2016,36(4):634-640.
[12]Ebrahimzadeh M A,Nabavi S M,Nabavi S F,et al.Antioxidant and free radical scavenging activity of H.officinalis L.Var.angustifolius,V.odorata,B hyrcana and C.speciosum[J].Pakistan Journal of Agricultural Sciences,2010,23(1):29-34.
[13]Bi Shufeng(毕淑峰),Zhang lingjie(张铃杰),Gao hui(高慧),et al.Chemical constituents and antioxidant activities in vitro of essential oils from Osmanthus fragrans fragrans[J].Modern Food Science and Technology(现代食品科技),2014,30(6):238-243,145.
[14]Wang Zongcheng(王宗成),Jiang Yuren(蒋玉仁),Liu Xiaowen(刘小文),et al.Optimization of extraction of total flavonoids from Zingiber officinale roscoe stem and leaves using response surface methodology and evaluation of its antioxidant activity[J].Nat Prod Res Dev(天然产物研究与开发),2015,27(9):1582-1588.
[15]Wu Hao(兀浩).Study on the extraction of dioscin with the surfactant and its hydrolysis process from Dioscorea Zingiberensis C.H.wright[D].Xi'an:Shaanxi University of Science and Technology(陕西科技大学),2013.
[16]Yang Huan(杨欢),Yan Zhinong(闫志农),Lu Xiaoli(卢晓黎).Surfactant assistant ultrasonic extraction of flavonoids from peanut[J].China Oils and Fats(中国油脂),2012,32(12):57-60.
[17]Yu Yali(于亚莉),Gao Feng(高峰),Liu Jingbo(刘静波),et al.Extraction of polyphenol from husk of earthnut by ultrasound[J].Food Science(食品科学),2007,28(1):257-260.
[18]Tang Haoguo(唐浩国).Study on flavanoids[M].Beijing:Science Press(科学出版社),2008:53.
[19]Prakash M J,Manikandan S,Mekala V.Modeling and optimization of betalain extration from Opuntiaficus-indica using Box-Behnken design with desirability function[J].Industrial Crops and Products,2013,49(8):304-311.
[20]Yu Shankai(于善凯),Zhang Ying(张英).Comparison of varieties of Chrysanthemum phenolic contents and free radical-scavenging capacity[J].Food Science(食品科学),2001,22(4):84-87.图2
[2]Parzonko A,Naruszewicz M.Cardioprotective effects of Aronia melanocarpa anthocynanins.from laboratory experiments to clinical practice[J].Current Pharmaceutical Design,2016,22(2):174-179.
[3]Kang N J,Shin S H,Lee H J,et al.Polyphenols as small molecular inhibitors of signaling cascades in carcinogenesis[J].Pharmacology&Therapeutics,2011,130(3):310-324.
[4]Tang Hehua(汤鹤华).Extraction of the total polyphenolic compounds from Florist Chrysanthemum on by ultrasonic aid[J].Jiangxi Chemical Industry(江西化工),2007,(4):110-112.
[5]Gong Jinyan(龚金炎),Ge Qing(葛青),Cheng Lichun(陈丽春)et al.Optimization extraction of polyphenols from Hangzhou white Chrysanthemum[J].Food Science and Technology(食品科技),2013,38(10):224-227.
[6]Kuang Chunlin(邝春林).Antioxidant,antibacterial activity of component extracted from Chrysanthemum morifolium Ramat.and Coreopsis tinctoria Nuff.[D]Ya'an:Sichuan Agricultural University(四川农业大学),2016.
[7]Li Yu(黎彧).Studies of the surfactan assisted microwave extraction of the pigment from Begonia fimbristipula Hance[J].Journal of Instrumental Analysis(分析测试学报),2005,24(4):95-97.
[8]Wang Yongsheng(王永胜).Determination and analysis of active substances in surfactant[J].Popular Standardzation(大众标准化),2013,(2):60-61.
[9]Chemat F,Huma Z,Khan M K.Applications of ultrasound in food technology:Processing,preservation and extraction[J].Ultrasonics Sonochemistry,2011,18(4):813-835.
[10]Wang Youqiong(王有琼),Ma Liyi(马李一),Zhang Chongquan(张重权),et al.Antioxidant capacity and extraction technique of polyphenol from the leaf of Azadirachta indica[J].Fine Chemicals(精细化工),2015,32(1):64-68.
[11]Zu Yuangang(祖元刚),Hu Yan(胡艳),Jiang Shougang(姜守刚).Extraction and anti-inflammatory of Korean pine polyphenol[J].Bulletin of Botanical Research(植物研究),2016,36(4):634-640.
[12]Ebrahimzadeh M A,Nabavi S M,Nabavi S F,et al.Antioxidant and free radical scavenging activity of H.officinalis L.Var.angustifolius,V.odorata,B hyrcana and C.speciosum[J].Pakistan Journal of Agricultural Sciences,2010,23(1):29-34.
[13]Bi Shufeng(毕淑峰),Zhang lingjie(张铃杰),Gao hui(高慧),et al.Chemical constituents and antioxidant activities in vitro of essential oils from Osmanthus fragrans fragrans[J].Modern Food Science and Technology(现代食品科技),2014,30(6):238-243,145.
[14]Wang Zongcheng(王宗成),Jiang Yuren(蒋玉仁),Liu Xiaowen(刘小文),et al.Optimization of extraction of total flavonoids from Zingiber officinale roscoe stem and leaves using response surface methodology and evaluation of its antioxidant activity[J].Nat Prod Res Dev(天然产物研究与开发),2015,27(9):1582-1588.
[15]Wu Hao(兀浩).Study on the extraction of dioscin with the surfactant and its hydrolysis process from Dioscorea Zingiberensis C.H.wright[D].Xi'an:Shaanxi University of Science and Technology(陕西科技大学),2013.
[16]Yang Huan(杨欢),Yan Zhinong(闫志农),Lu Xiaoli(卢晓黎).Surfactant assistant ultrasonic extraction of flavonoids from peanut[J].China Oils and Fats(中国油脂),2012,32(12):57-60.
[17]Yu Yali(于亚莉),Gao Feng(高峰),Liu Jingbo(刘静波),et al.Extraction of polyphenol from husk of earthnut by ultrasound[J].Food Science(食品科学),2007,28(1):257-260.
[18]Tang Haoguo(唐浩国).Study on flavanoids[M].Beijing:Science Press(科学出版社),2008:53.
[19]Prakash M J,Manikandan S,Mekala V.Modeling and optimization of betalain extration from Opuntiaficus-indica using Box-Behnken design with desirability function[J].Industrial Crops and Products,2013,49(8):304-311.
[20]Yu Shankai(于善凯),Zhang Ying(张英).Comparison of varieties of Chrysanthemum phenolic contents and free radical-scavenging capacity[J].Food Science(食品科学),2001,22(4):84-87.图2