槲皮素的制备及其体外抗细胞损伤作用初探
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摘要
目的:探讨槲皮素对对乙酰氨基酚诱导的人肝细胞LO2的抗氧化作用及可能的凋亡机制。方法:采用碱沉淀酸水解法从槐米提取分离出槲皮素,通过CCK8法测定0-160mM的槲皮素对人正常肝细胞(LO2细胞)增殖的影响,采用Western blot检测槲皮素对APAP诱导LO2细胞损伤模型凋亡蛋白Caspase-9的表达的影响,结果:槲皮素在实验浓度范围内对LO2细胞没有细胞毒性,槲皮素各剂量组的Caspase-9表达呈现下调趋势。结论:槲皮素对APAP氧化损伤的肝细胞具有保护作用,其机制可能与下调的Caspase-9表达有关。
Objective: To investigate the antioxidant effect and the possible apoptosis mechanism of quercetin on human norma hepatocyte LO2 cells injured by APAP. Methods: Injury model with LO2 cells was established and induced by APAP, and investigated the effect within 160 mM quercetin, the proliferation of the cells was determined by CCK8 assay. The expression of the apoptotic protein caspase-9 was detected by Western blot. Results: Quercetin did not exert cytotoxic effects on LO2 cells in the concentration range of 0-160 m M. Compared with the model group, the expression of caspase-9 in the quercetin group was downregulated. Conclusion: Quercetin has protective effects on APAP oxidatively injured LO2 cells, and its mechanism might be related to down-regulated expression of caspase-9.
Objective: To investigate the antioxidant effect and the possible apoptosis mechanism of quercetin on human norma hepatocyte LO2 cells injured by APAP. Methods: Injury model with LO2 cells was established and induced by APAP, and investigated the effect within 160 mM quercetin, the proliferation of the cells was determined by CCK8 assay. The expression of the apoptotic protein caspase-9 was detected by Western blot. Results: Quercetin did not exert cytotoxic effects on LO2 cells in the concentration range of 0-160 m M. Compared with the model group, the expression of caspase-9 in the quercetin group was downregulated. Conclusion: Quercetin has protective effects on APAP oxidatively injured LO2 cells, and its mechanism might be related to down-regulated expression of caspase-9.
引文
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[2]马纳,李亚静,范吉平.槲皮素药理作用研究进展[J].辽宁中医药大学学报,2018,20(8):221-224
[3]沈钦海,秦召敏,刘丽,等.槲皮素对肝细胞氧化应激损伤的保护作用[J].天津医药,2015,43(10):1100-1103.
[4]吴黉坦,张友珍,黄立森,等.金边桑叶多酚提取物抗氧化及肝保护作用研究[J].天然产物研究与开发,2018,30(6):944-950.
[5]杨志刚,潘龙银,王心睿.苦瓜甾醇对对乙酰氨基酚致小鼠肝损伤的保护作用[J].天然药物研究与开发,2015,27(12):2031-2034.
[6]Deshi,Dong,Lina,Xu,Xu,et al.Effects of the total saponins from Rosa laevigata Michx fruit against acetaminophen-induced liver damage in mice via induction of autophagy and suppression of inflammation and apoptosis[J].Molecules(Basel,Switzerland),2014,19(6):7189-206.
[7]石元英,邓立东,饶伟文,等.牡荆素对人肝细胞癌SMMC-7721细胞的增殖抑制作用及其机制的影响[J].中国医院药学杂志,2016,36(5):366-371.
[8]赵文彬,刘金荣,樊莲莲,刘桂花.从槐米中提取槲皮素方法的研究[J].石河子大学学报(自然科学版),2006(3):302-304.