摘要
目的提高酒女贞子配方颗粒质量标准控制。方法采用薄层色谱法,以女贞子对照药材作为对照进行定性鉴别;采用高效液相色谱法测定其特征成分特女贞苷含量。结果薄层色谱鉴别中,供试品溶液色谱在与对照药材溶液色谱相应的位置上,显相同颜色的荧光斑点。特女贞苷在进样量为0. 766 46~15. 329 19μg范围内与峰面积线性关系良好(r2=0. 999 8);平均回收率为91. 70%,RSD为1. 14%(n=6)。结论该方法简便、准确,重复性良好,完善和提高了酒女贞子配方颗粒的质量标准。
Objective To improve the quality control standard of Wine-Fried Ligustri Lucidi Fructus Dispensing Granules. Methods TLC method was used for qualitative identification with Ligustri Lucidi Fructus as the reference crude drug,and HPLC method was used for content determination of specnuezhenide in Wine-Fried Ligustri Lucidi Fructus Dispensing Granules. Results In TLC identification,the fluorescence spots in the chromatogram obtained with the test solution corresponded in position and color with the spots in the chromatogram obtained with the reference crude drug solution. The specnuezhenide showed good linear relationship with peak area in the range of 0. 766 46-15. 329 19 μg( r2 = 0. 999 8),the average recovery rate was 91. 70% and RSD was 1. 14%( n = 6). Conclusion The method is simple,accurate and reproducible,which can improve the quality standard of Wine-Fried Ligustri Lucidi Fructus Dispensing Granules.
引文
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