基于ICP-MS法对不同产地酸枣仁中微量元素的主成分分析
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摘要
比较河北、山东、河南、辽宁、陕西等13个不同产地酸枣仁中Mg、Mn、Fe、Zn、Cu、Cd、Hg和Pb微量元素的含量。采用微波消解技术处理样品,采用电感耦合等离子体质谱法(inductively coupled plasma mass spectrometry,ICPMS)测定13个不同产地酸枣仁中8种微量元素的含量变化;并利用SPSS统计软件中主成分分析对测定的结果进行综合分析。通过添加标准品进行回收试验,结果表明该方法的回收率在91.85%～99.67%之间,相对标准偏差(relative standard deviation,RSD)均小于2.24%。不同产地酸枣仁中对人体有益的元素Mg、Mn、Fe、Zn含量普遍较丰富;有害的重金属元素Cu、Cd、Hg、Pb含量均在限量标准范围内。主成分分析结果显示:13个不同产地酸枣仁可分为4类,前两个主成分的总贡献率达到72.14%。ICP-MS法具有同时进行多种元素测定、准确度高等优点,该测定结果为评价市售酸枣仁品种特征提供一定的数据支撑。
To compare the contents of trace elements in Zizyphi spinosae semen from thirteen different growing areas,including Hebei,Shandong,Henan,Liaoning,and Shaanxi Province. Microwave Digestion-ICP-MS method was used for the determination of eight trace elements(Mg,Mn,Fe,Zn,Cu,Cd,Hg,and Pb)and their data were analyzed by the principal components analysis in combination with SPSS statistical software. The results showed that the relative standard deviation(RSD)was less than 2.24 % for all the elements,and the recovery was between 91.85 % and 99.67 % by addition standard recovery experiment. The Zizyphi spinosae semen of different areas had different trace elements content. The content of trace elements(Mg,Mn,Fe and Zn)beneficial to the human body was rich,and the content of the heavy metal trace elements(Cu,Cd,Hg and Pb)were within the limits of the standard range. The results of principal components analysis was consistent.The principal components analysis results showed that 72.14 % of the contribution rate contributed to the two principal components(Mg,Mn). ICP-MS could satisfy the sample examination request and provide scientific rationale for determining inorganic elements in Zizyphi spinosae semen.
To compare the contents of trace elements in Zizyphi spinosae semen from thirteen different growing areas,including Hebei,Shandong,Henan,Liaoning,and Shaanxi Province. Microwave Digestion-ICP-MS method was used for the determination of eight trace elements(Mg,Mn,Fe,Zn,Cu,Cd,Hg,and Pb)and their data were analyzed by the principal components analysis in combination with SPSS statistical software. The results showed that the relative standard deviation(RSD)was less than 2.24 % for all the elements,and the recovery was between 91.85 % and 99.67 % by addition standard recovery experiment. The Zizyphi spinosae semen of different areas had different trace elements content. The content of trace elements(Mg,Mn,Fe and Zn)beneficial to the human body was rich,and the content of the heavy metal trace elements(Cu,Cd,Hg and Pb)were within the limits of the standard range. The results of principal components analysis was consistent.The principal components analysis results showed that 72.14 % of the contribution rate contributed to the two principal components(Mg,Mn). ICP-MS could satisfy the sample examination request and provide scientific rationale for determining inorganic elements in Zizyphi spinosae semen.
引文
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[2]陈莺,陈少玫.酸枣仁汤临床应用及药理实验研究进展[J].山西中医,2014,30(12):41-43
[3]张巧月,史贺,杨浩天,等.药食两用酸枣仁研究近况与开发前景[J].时珍国医国药,2016(6):1476-1479
[4]康阿龙,汤迎爽.酸枣仁及混伪品的鉴别[J].陕西中医杂志,1998(9):420
[5]曲彩红,章钧,黎小妍,等.高效液相色谱-串联质谱法测定酸枣仁中酸枣仁皂苷A[J].中成药,2013,35(10):2209-2212
[6]于雁灵,汪学昭,王运革.酸枣根与酸枣仁中部分微量元素的比较研究[J].广东微量元素科学杂志,1999,6(12):50-52
[7]陈雯,黄世敬.酸枣仁化学成分及药理作用研究进展[J].时珍国医国药,2011,22(7):1726-1728
[8]陈科先,赵丽梅,嵇长久,等.酸枣仁中的黄酮碳苷类成分研究[J].中国中药杂志,2015,40(8):1503-1507
[9]袁珂,薛月芹,桂仁意,等.微波消解-原子吸收光谱法测定不同产地淡竹叶中微量元素的含量[J].光谱学与光谱分析,2010,30(3):804-808
[10]舒薇,高秋,周芥锋,等.火焰原子吸收光谱法测定中药莪术及姜黄中的微量元素含量[J].微量元素与健康研究,2015,31(6):34-35
[11]付娟,张海弢,杨素德,等.基于ICP-MS法分析九味熄风颗粒中25种重金属及微量元素[J].中草药,2015,46(21):3185-3189
[12]张勇仓,刘兰,达娃,等.原子吸收光谱法测定二十五味珊瑚丸中微量元素铁锌的含量[J].微量元素与健康研究,2017(1):32-34
[13]郭鹏然,潘佳钏,雷永乾,等.微波等离子体原子发射光谱新技术同时测定环境水样中多种元素[J].分析化学,2015,43(5):748-753
[14]高加龙,章超桦,梁家辉,等.湿法消化-石墨炉原子吸收法测定贝肉中镉含量的条件优化[J].现代食品科技,2012,28(11):1585-1589
[15]杨雁芳,张友波,杨秀伟.基于微波消解的ICP-OES和ICP-MS法测定中药独活中的24种微量元素[J].药物分析杂志,2016(11):2004-2008
[16]黄长友.中药微量元素的主成分分析[J].广东微量元素科学杂志,2007,14(4):39-42
[17]申明金,曹洪斌.降脂中药微量元素的主成分分析和聚类分析[J].化学分析计量,2016,25(1):26-29