来自安徽不同地区黄瓜绿斑驳花叶病毒(CGMMV)cp基因的分子进化分析
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摘要
为了揭示黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)安徽分离物分子变异及系统进化情况,从安徽省5个地区采集感染CGMMV的葫芦科样本。提取感病样本总RNA,经RT-PCR扩增、克隆和测序,获得17个CGMMV分离物的cp基因序列。序列比对发现,17个CGMMV安徽分离物的cp基因核苷酸序列相似性极高,达98.4%~100%,与中国及东亚国家和地区的各个CGMMV分离物cp基因核苷酸序列相似性也非常高,达98.6%~100%,而与CGMMV西班牙分离物和俄罗斯分离物cp基因核苷酸序列相似性相对较低,为90.7%~92.6%。从构建的系统关系树可以看出,17个CGMMV安徽分离物与中国及东亚国家和地区的各个CGMMV分离物形成1个单独的分支,而CGMMV俄罗斯与西班牙分离物聚成另1个分支,说明来源于中国和东亚的CGMMV亲缘关系较近。
In order to reveal the molecular variation and phylogenetic evolution of Cucumber green mottle mosaic virus(CGMMV) isolated from Anhui Province, Cucurbitaceae plant samples infected with CGMMV were collected from five regions in Anhui province. Total RNA was extracted from the samples, and cp genes of 17 CGMMV isolates were obtained by RT-PCR amplification, cloning and sequencing. Sequence comparison revealed that cp genes of 17 CGMMV isolates of Anhui province shared a extremely high sequence similarity(98.4%-100%), and also had very high sequence similarity(98.6%-100%) with cp genes of CGMMV isolates from China and other countries and regions of East Asia, and had relatively lower sequence similarity(90.7%-92.6%) with cp genes of CGMMV isolates from Spain and Russia. It was indicated from the phylogenetic tree that 17 CGMMV isolates of Anhui province and CGMMV isolates from China and other countries and regions of East Asia clustered into a separate branch, and CGMMV isolates from Spain and Russia clustered into another branch. It was illustrated that the CGMMV from China and East Asia had a very close relationship.
In order to reveal the molecular variation and phylogenetic evolution of Cucumber green mottle mosaic virus(CGMMV) isolated from Anhui Province, Cucurbitaceae plant samples infected with CGMMV were collected from five regions in Anhui province. Total RNA was extracted from the samples, and cp genes of 17 CGMMV isolates were obtained by RT-PCR amplification, cloning and sequencing. Sequence comparison revealed that cp genes of 17 CGMMV isolates of Anhui province shared a extremely high sequence similarity(98.4%-100%), and also had very high sequence similarity(98.6%-100%) with cp genes of CGMMV isolates from China and other countries and regions of East Asia, and had relatively lower sequence similarity(90.7%-92.6%) with cp genes of CGMMV isolates from Spain and Russia. It was indicated from the phylogenetic tree that 17 CGMMV isolates of Anhui province and CGMMV isolates from China and other countries and regions of East Asia clustered into a separate branch, and CGMMV isolates from Spain and Russia clustered into another branch. It was illustrated that the CGMMV from China and East Asia had a very close relationship.
引文
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[12]杜江,成媛媛,牛二波,等.黄瓜绿斑驳花叶病毒山西西瓜分离物外壳蛋白基因序列分析[J].植物保护,2015,41(2):142-145.
[13]TETSUO M,RYOITI K,TAKESHI O,et al.Nucleotide sequence of the coat protein cistron and the 3′noncoding region of Cucumber green mottle mosaic virus(watermelon strain)RNA[J].Virology,1983,127(1):54-64.
[14]ALI M E,YUTAKA T,KAPPEI K,et al.Molecular analysis of transgenic melon plants showing virus resistance conferred by direct repeat of movement gene of Cucumber green mottle mosaic virus[J].Plant Cell Rep,2012,31:1371-1377.
[2]冯兰香,谢丙炎,杨宇红,等.检疫性黄瓜绿斑驳花叶病毒的检测和防疫控制[J].中国蔬菜,2007(9):34-38.
[3]秦碧霞,蔡健和,刘志明,等.侵染观赏南瓜的黄瓜绿斑驳花叶病毒的初步鉴定[J].植物检疫,2005,19(4):198-200.
[4]王峰,任春梅,季英华,等.黄瓜绿斑驳花叶病毒海南分离物基因组测定与毒源分析[J].植物保护,2014,40(6):75-81.
[5]宋顺华,吴萍,宫国义,等.黄瓜绿斑驳花叶病的发生及防控策略[J].植物保护,2014(8):105-108.
[6]周玲玲,吴元华,赵秀香,等.黄瓜绿斑驳花叶病毒生物学特性及对西瓜生长的影响[J].沈阳农业大学学报,2008,39(4):417-422.
[7]YOON J Y,MIN B E,CHOI S H,et al.Completion of nucleotide sequence and generation of highly infec tious transcripts to cucurbits from full-length c DNA clone of Kyuri green mottle mosaic virus[J].Arch Virol,2001,146(11):2085-2096.
[8]LIU H W,LUO L X,LI J Q,et al.Pollen and seed transmission of Cucumber green mottle mosaic virus in cucumber[J].Plant Pathol,2014,63(1):72-77.
[9]田永蕾,刘冬梅,张永江,等.黄瓜绿斑驳花叶病毒北京和山东分离物生物学测定及其基因组比较[J].植物检疫,2009,23(6):1-6.
[10]钟敏,赵绪生,何乙坤,等.黄瓜绿斑驳病毒河北分离物外壳蛋白基因序列分析及其分组[J].河北大学学报,2015,38(1):92-97.
[11]陈红运,林石明,陈青,等.黄瓜绿斑驳花叶病毒辽宁分离物全基因组序列测定[J].病毒学报,2009,25(1):68-72.
[12]杜江,成媛媛,牛二波,等.黄瓜绿斑驳花叶病毒山西西瓜分离物外壳蛋白基因序列分析[J].植物保护,2015,41(2):142-145.
[13]TETSUO M,RYOITI K,TAKESHI O,et al.Nucleotide sequence of the coat protein cistron and the 3′noncoding region of Cucumber green mottle mosaic virus(watermelon strain)RNA[J].Virology,1983,127(1):54-64.
[14]ALI M E,YUTAKA T,KAPPEI K,et al.Molecular analysis of transgenic melon plants showing virus resistance conferred by direct repeat of movement gene of Cucumber green mottle mosaic virus[J].Plant Cell Rep,2012,31:1371-1377.