醋延胡索饮片及其颗粒剂的谱-效关系
|
摘要
目的探讨醋延胡索饮片及其颗粒剂HPLC指纹图谱与镇痛药效之间的相关性,即谱-效关系。方法建立醋延胡索饮片及其颗粒剂HPLC指纹图谱,对两者的化学成分组成进行差异性分析。采用热板法和醋酸扭体法评价镇痛药效,采用灰色关联度分析法研究延胡索的谱-效关系。结果建立了不同批次醋延胡索饮片、颗粒剂的HPLC指纹图谱,其中饮片确定了15个共有峰,颗粒剂确定了14个共有峰。醋延胡索饮片及其颗粒剂均能提高小鼠痛阈值,以低剂量颗粒剂痛阈提高百分率最为显著。镇痛谱-效关联度从高到低依次为(峰号) 7> 6> 5> 11> 13> 14> 15> 12> 4> 10> 9> 3> 2> 1,并通过对照品指认了5个相关度较大的色谱峰。结论通过谱-效关系明确了延胡索镇痛作用是由原阿片碱、延胡索甲素、延胡索乙素、小檗碱和巴马汀等多种成分共同作用的结果。
AIM To study the relationship between the chemical constituents and the analgesic effect of Corydalis pieces and granules. METHODS HPLC fingerprints and chemical compositions of Corydalis pieces and granules was established and analyzed. The analgesic effect of Corydalis pieces and granules was observed by mice hot-board and acetic-acid-induced twisting experiments. The spectrum-effect relationship was studied by gray relational analysis after statistical analysis. RESULTS The HPLC fingerprints of different batches of Corydalis pieces and granules were established,fifteen common peaks were identified in the pieces,and fourteen common peaks were identified in the granules. The analgesic effect results showed that Corydalis pieces and granules could increase the pain threshold,with low dose Corydalis granules increased significantly. The analgesic spectrum-effect correlation result was as following order(peak) 7 > 6 > 5 > 11 > 13 > 14 > 15 > 12 > 4 > 10 > 9 > 3 > 2 > 1,and the top five peaks with strong contribution to analgesic effects were identified. CONCLUSION The analgesic effect of Corydalis pieces and granules is the result of combination of various components,such as protopine,tetrahydropalmatine,corydaline,berberine and palmatine.
AIM To study the relationship between the chemical constituents and the analgesic effect of Corydalis pieces and granules. METHODS HPLC fingerprints and chemical compositions of Corydalis pieces and granules was established and analyzed. The analgesic effect of Corydalis pieces and granules was observed by mice hot-board and acetic-acid-induced twisting experiments. The spectrum-effect relationship was studied by gray relational analysis after statistical analysis. RESULTS The HPLC fingerprints of different batches of Corydalis pieces and granules were established,fifteen common peaks were identified in the pieces,and fourteen common peaks were identified in the granules. The analgesic effect results showed that Corydalis pieces and granules could increase the pain threshold,with low dose Corydalis granules increased significantly. The analgesic spectrum-effect correlation result was as following order(peak) 7 > 6 > 5 > 11 > 13 > 14 > 15 > 12 > 4 > 10 > 9 > 3 > 2 > 1,and the top five peaks with strong contribution to analgesic effects were identified. CONCLUSION The analgesic effect of Corydalis pieces and granules is the result of combination of various components,such as protopine,tetrahydropalmatine,corydaline,berberine and palmatine.
引文
[1]国家药典委员会中华人民共和国药典:一部[S].北京:中国医药科技出版社,2015:139.
[2]鲁春梅,张春森,姜立勇.延胡索化学成分及药理作用研究进展[J].中国现代药物应用,2011,05(15):126.
[3]杨鑫宝,杨秀伟,刘建勋.延胡索物质基础研究[J].中国中药杂志,2014,39(1):20.
[4]张斐姝,蔡舒婷,舒忻,等.中药配方颗粒的临床运用概况与未来趋势[J].中国医药导报,2016,13(16):70.
[5]魏梅,杜兰哲,李慧,等.醋延胡索饮片、水煎液、配方颗粒高效液相色谱特征图谱相关性研究[J].中药材,2015,38(5):1066.
[6]WEI F,HU Q,HUANG J,et al. Screening active compounds from Corydalis yanhusuo by combining high expression VEGF receptor HEK293 cell membrane chromatography with HPLC-ESI-IT-TOFMSn method.[J]. J Pharmaceut Biomed,2017,136:134.
[7]国家药品监督管理局.中药注射剂指纹图谱研究的技术要求(暂行)[J].中成药,2000,22(10):671.
[8]林梦雅,张玉萍,李雅,等.基于灰色关联度分析的丹参提取物抗炎作用谱效关系研究[J].中草药,2017,48(16):3447.
[9]许俊洁,卢金清,李肖爽,等.神农香菊挥发油体外抗氧化活性的谱效关系研究[J].药物分析杂志,2016,(10):1823.
[2]鲁春梅,张春森,姜立勇.延胡索化学成分及药理作用研究进展[J].中国现代药物应用,2011,05(15):126.
[3]杨鑫宝,杨秀伟,刘建勋.延胡索物质基础研究[J].中国中药杂志,2014,39(1):20.
[4]张斐姝,蔡舒婷,舒忻,等.中药配方颗粒的临床运用概况与未来趋势[J].中国医药导报,2016,13(16):70.
[5]魏梅,杜兰哲,李慧,等.醋延胡索饮片、水煎液、配方颗粒高效液相色谱特征图谱相关性研究[J].中药材,2015,38(5):1066.
[6]WEI F,HU Q,HUANG J,et al. Screening active compounds from Corydalis yanhusuo by combining high expression VEGF receptor HEK293 cell membrane chromatography with HPLC-ESI-IT-TOFMSn method.[J]. J Pharmaceut Biomed,2017,136:134.
[7]国家药品监督管理局.中药注射剂指纹图谱研究的技术要求(暂行)[J].中成药,2000,22(10):671.
[8]林梦雅,张玉萍,李雅,等.基于灰色关联度分析的丹参提取物抗炎作用谱效关系研究[J].中草药,2017,48(16):3447.
[9]许俊洁,卢金清,李肖爽,等.神农香菊挥发油体外抗氧化活性的谱效关系研究[J].药物分析杂志,2016,(10):1823.