芒果苷对自发性高血压大鼠交感神经形态学及MCP-1/CCR2信号通路的影响
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摘要
目的 :通过观察芒果苷对自发性高血压大鼠交感神经形态学及单核细胞趋化蛋白-1(MCP-1)及其受体CCR2表达量的影响,探讨自发性高血压大鼠是否存在交感神经炎症损伤以及芒果苷可能的抗炎药效及机制。方法 :以8只10周龄正常血压对照大鼠(WKY)作为空白组,以40只同龄SHR大鼠作为研究对象,随机分为模型组、苯那普利组、芒果苷高剂量组、芒果苷中剂量组和芒果苷低剂量组。各组动物按每天给药10 m L/(kg·d)连续灌胃,灌胃给药2个月后停药,杀鼠前禁食12 h。结果 :各组大鼠交感神经均未见明显病理形态学改变;免疫组化检测结果显示:与空白对照组比较,模型组交感神经MCP-1、CCR2水平显著升高(P<0.01);与模型组比较,各组给药大鼠交感神经组织中MCP-1、CCR2含量均有不同程度下降(P<0.05,P<0.01)。结论 :自发性高血压大鼠存在交感神经炎症损伤。芒果苷对自发性高血压大鼠交感神经组织病理形态学无显著影响,但可下调大鼠交感神经组织异常升高的炎症因子MCP-1、CCR2水平,对交感神经炎症损伤具有一定的保护作用。
Objective :By observing the mangiferin on sympathetic nerve morphology in spontaneously hypertensive rats and the expression of monocyte chemoattractant protein-1(MCP-1)and its receptor CCR2,to investigate the existence of spontaneously hypertensive rats and mechanism of anti-inflammatory effect of sympathetic nerve injury and inflammation of mangiferin may.Methods :By using 8 normal blood pressure at 10 weeks of age WKY rats served as normal controls.40 spontaneously hypertensive rats(SHR)were randomly divided into model group,benazepril group,the mangiferin-high dose group,the mangiferin middle dose group,the mangiferin low dose group.Animals in each group were given 10 m L/(kg·d)for days,and the rats were fed for a period of 2 months.Results :There were no obvious pathological changes in the sympathetic nerves of the rats in each group,the results of immunohistochemistry showed that :compared with the blank control group,the levels of MCP-1 and CCR2 were significantly increased in the model group(P<0.01);compared with the model group,the contents of MCP-1 and CCR2 were decreased in different groups(P<0.05,P<0.01).Conclusion :There are sympathetic nerve injury in spontaneously hypertensive rats.Mangiferin has no significant effect on sympathetic nerve tissue and pathological morphology of spontaneously hypertensive rats,but can decrease the rat sympathetic nerve tissue abnormal elevation of inflammatory cytokines MCP-1 and CCR2,has a protective effect on sympathetic nerve injury.
Objective :By observing the mangiferin on sympathetic nerve morphology in spontaneously hypertensive rats and the expression of monocyte chemoattractant protein-1(MCP-1)and its receptor CCR2,to investigate the existence of spontaneously hypertensive rats and mechanism of anti-inflammatory effect of sympathetic nerve injury and inflammation of mangiferin may.Methods :By using 8 normal blood pressure at 10 weeks of age WKY rats served as normal controls.40 spontaneously hypertensive rats(SHR)were randomly divided into model group,benazepril group,the mangiferin-high dose group,the mangiferin middle dose group,the mangiferin low dose group.Animals in each group were given 10 m L/(kg·d)for days,and the rats were fed for a period of 2 months.Results :There were no obvious pathological changes in the sympathetic nerves of the rats in each group,the results of immunohistochemistry showed that :compared with the blank control group,the levels of MCP-1 and CCR2 were significantly increased in the model group(P<0.01);compared with the model group,the contents of MCP-1 and CCR2 were decreased in different groups(P<0.05,P<0.01).Conclusion :There are sympathetic nerve injury in spontaneously hypertensive rats.Mangiferin has no significant effect on sympathetic nerve tissue and pathological morphology of spontaneously hypertensive rats,but can decrease the rat sympathetic nerve tissue abnormal elevation of inflammatory cytokines MCP-1 and CCR2,has a protective effect on sympathetic nerve injury.
引文
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[10]胡小勤,邓家刚,杜正彩,等.芒果苷联合葛根素对自发性高血压大鼠心脏炎性损伤的协同保护作用[J].中国实验方剂学杂志,2017,23(9):115-120.
[11]胡小勤,杜正彩,李琦,等.芒果苷联合葛根素对自发性高血压大鼠肾脏炎性损伤的协同保护作用[J].科学技术与工程,2016(32):196-204.
[12]张迎怡,陈树涛,丛洪良.MCP-1及其基因多态性与原发性高血压的关系[J].山东医药,2015(39):93-94.
[13]何军,陈仪,肖恒怡,等.醋柳黄酮对自发性高血压大鼠主动脉内MCP-1表达的影响[J].四川大学学报:医学版,2009(3):481-485.
[14]董玉林,曹德利,张志军,等.小鼠杏仁核脑区(Ce A)中单核细胞趋化因子(MCP-1)及其受体CCR2在慢性炎症性疼痛过程中的作用[J].复旦学报:医学版,2015(1):57-61,100.
[2]王春乐,陈炜,唐晓鸿.交感神经功能的评价方法[J].中华高血压杂志,2014(9):888-891.
[3]杨慧美,晁满香,赵雅茹,等.交感神经过度兴奋对慢性肾脏病伴随高血压作用机制研究进展[J].神经解剖学杂志,2015(4):520-522.
[4]陈志雄,杨天伦,陈美芳,等.高血压病患者血管内皮功能和单核细胞趋化蛋白1变化的临床意义[J].中国动脉硬化杂志,2007(2):125-128.
[5]张玮,曾群英,郑东诞,等.老年高血压病患者血浆MCP-1、GMP-140、v WF含量的研究[J].实用医学杂志,2006(2):152-153.
[6]蔡智刚,唐新征,张毅,等.MCP-1/CCR2在老年高血压不同证型中的表达[J].临床合理用药杂志,2013,6(23):88-89.
[7]吴进寿,洪华山.高血压与炎症关系的研究进展[J].中国临床药理学与治疗学,2006(2):136-140.
[8]Jordan J.Sympathetic genes,baroreflexes,and hypertension[J].Clin Auton Res,2004,14(6):358-359.
[9]贺若曦,苏晓丽,向永红,等.不同低氧模式对大鼠交感神经活性的影响机制及其与血压变化的关系[J].中南大学学报:医学版,2011(10):1003-1007.
[10]胡小勤,邓家刚,杜正彩,等.芒果苷联合葛根素对自发性高血压大鼠心脏炎性损伤的协同保护作用[J].中国实验方剂学杂志,2017,23(9):115-120.
[11]胡小勤,杜正彩,李琦,等.芒果苷联合葛根素对自发性高血压大鼠肾脏炎性损伤的协同保护作用[J].科学技术与工程,2016(32):196-204.
[12]张迎怡,陈树涛,丛洪良.MCP-1及其基因多态性与原发性高血压的关系[J].山东医药,2015(39):93-94.
[13]何军,陈仪,肖恒怡,等.醋柳黄酮对自发性高血压大鼠主动脉内MCP-1表达的影响[J].四川大学学报:医学版,2009(3):481-485.
[14]董玉林,曹德利,张志军,等.小鼠杏仁核脑区(Ce A)中单核细胞趋化因子(MCP-1)及其受体CCR2在慢性炎症性疼痛过程中的作用[J].复旦学报:医学版,2015(1):57-61,100.