蒙药复方协日嘎-4体内外化学成分分析
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摘要
目的建立蒙药复方协日嘎-4体内外HPLC特征图谱,归属并鉴定其体内外化学成分。方法取SD大鼠,灌胃一定量的蒙药复方协日嘎-4制备体内含药血清和代谢尿液,通过HPLC法进行测定,比对含药血清和尿液中有效组分,建立蒙药复方协日嘎-4体内外HPLC特征图谱。结果分析蒙药复方协日嘎-4体外HPLC特征图谱,共发现了53个峰;蒙药复方协日嘎-4体内给药后出现了35个入血成分,25个为体外成分直接入血,推测另外10个为新生代谢产物。7、9、12、14、15、17、26、27、30、33、51、53号峰来源于姜黄,18、19号峰来源于黄柏,1、2、3、10、16号峰来源于栀子,8、18、31、32号峰来源于蒺藜。通过体内外化学成分比对鉴定出4个入血化学成分,其中3个与代谢中成分一致,推测其可能以原型成分发挥作用并排泄。结论入血成分及代谢产物可能是复方发挥药效的有效成分,为进一步探究蒙药复方协日嘎-4治疗疾病的机制提供依据。
Objective To establish the in vitro and in vivo HPLC profiles of Mongolian drug compound Xieriga-4,and assign and identify its chemical composition in vitro and in vivo. Methods To take SD rats and intragastrically administer a certain amount of Mongolian medicine compound Xieriga-4 to prepare in vivo serum and metabolic urine,and measure with HPLC. comparing the effective components in drug-containing serum and urine,an in vitro and in vivo HPLC profile of Mongolian drug compound Xieriga-4 was established. Results The in vitro HPLC profiles of Mongolian medicine compound Xieriga-4 were analyzed,and a total of 53 peaks were found. There were 35 blood components after in vivo administration of Mongolian Medicine Compound Xieriga-4,and 25 blood components were directly injected into the blood. The other 10 were considered new metabolites. Peaks 7,9,12,14,15,17,26,27,30,33,51,53 originated from turmeric,Peaks 18 and 19 came from Cork; the peaks 1,2,3,10,and 16 from lice; the peaks 8,18,31, and 32 from earthworms. Four incoming chemical constituents were identified through in vitro and in vivo chemical composition comparisons. Three of them were identical to those in metabolism and it was assumed that they may act as prototype components and be excreted. Conclusion Blood components and metabolites may be effective components of compound effects. This article speculates that the composition of blood components of the compound into the blood to speculate the mechanism of its treatment,in order to provide further evidence to explore the mechanism of Mongolian medicine compound Xieriga-4 treatment of diseases.
Objective To establish the in vitro and in vivo HPLC profiles of Mongolian drug compound Xieriga-4,and assign and identify its chemical composition in vitro and in vivo. Methods To take SD rats and intragastrically administer a certain amount of Mongolian medicine compound Xieriga-4 to prepare in vivo serum and metabolic urine,and measure with HPLC. comparing the effective components in drug-containing serum and urine,an in vitro and in vivo HPLC profile of Mongolian drug compound Xieriga-4 was established. Results The in vitro HPLC profiles of Mongolian medicine compound Xieriga-4 were analyzed,and a total of 53 peaks were found. There were 35 blood components after in vivo administration of Mongolian Medicine Compound Xieriga-4,and 25 blood components were directly injected into the blood. The other 10 were considered new metabolites. Peaks 7,9,12,14,15,17,26,27,30,33,51,53 originated from turmeric,Peaks 18 and 19 came from Cork; the peaks 1,2,3,10,and 16 from lice; the peaks 8,18,31, and 32 from earthworms. Four incoming chemical constituents were identified through in vitro and in vivo chemical composition comparisons. Three of them were identical to those in metabolism and it was assumed that they may act as prototype components and be excreted. Conclusion Blood components and metabolites may be effective components of compound effects. This article speculates that the composition of blood components of the compound into the blood to speculate the mechanism of its treatment,in order to provide further evidence to explore the mechanism of Mongolian medicine compound Xieriga-4 treatment of diseases.
引文
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[2]H Iwama,S Amagaya,Y Ogihara. Effect of shosaikoto,a Japanese and Chinese traditional herbal medicinal mixture,on the mitogenic activity of lipopolys-accharide:A new pharmacological testing method[J]. Journal of Ethnopharmacology,1987,21(1):45-53.
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[4]陈卓,潘明佳,邢雪飞,等.血清药物化学在中药及其复方药效物质基础研究中的进展[J].药物评价研究,2016,39(1):143-147.Chen Z,Pan MJ,Xing XF,et al. Progress in the study of serum drug chemistry in the basic research of traditional Chinese medicine and its pharmacodynamics[J]. Drug Evaluation Research,2016,39(1):143-147.
[5]张灵娜,林兵,宋洪涛.中药血清药理学、血清药物化学的研究概况及展望[J].中草药,2015,46(17):2662-2666.Zhang LN,Lin B,Song HT. Summary and prospect of studies on serum pharmacology and serum medicinal chemistry of Chinese herbs[J]. Chinese Traditional and Herbal Drugs,2015,46(17):2662-2666.