蒙药复方协日嘎-4在正常和前列腺炎症模型大鼠血清中总生物碱的含量比较研究
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摘要
通过灌胃给予正常组和完全弗氏佐剂致前列腺炎症模型组大鼠相同剂量的蒙药复方协日嘎-4提取液,采用紫外可见分光光度法,以小檗碱为对照品,在419nm波长处测定大鼠血清中总生物碱吸光度,计算并比较蒙药复方协日嘎-4在正常和完全弗氏佐剂致前列腺炎症模型大鼠血清中总生物碱的含量.结果显示3个批次正常组大鼠血清中总生物碱的平均含量分别是0.0639mg.mL-1、0.0658mg.mL-1、0.0673mg.mL-1;3个批次完全弗氏佐剂致前列腺炎症模型组大鼠血清中总生物碱的平均含量分别是0.0546mg.mL-1、0.0569mg.mL-1、0.0594mg.mL-1.说明蒙药复方协日嘎-4中总生物碱类成份对非细菌性前列腺炎症大鼠有一定的调控作用,为蒙药复方协日嘎-4治疗非细菌性前列腺炎提供一定的科学依据.
The normal group and complete Frederian adjuvant-induced prostatein flammation model group rats were gavaged with the same dose of Mongolian medicine compound Xieriga-4 extract.The absorbance of total terpene in rat serum at 419 nm wavelength was monitored using UVvis spectrophotometer with the berberine as the standard.The contents of total alkaloids in the serum of normal and complete Frederian adjuvant-induced prostate inflammation model rats gavaged with Mongolian medicine compound Xieriga-4 were calculated.The results showed that the contents of total alkaloids in the serum of three batches of normal rats were determined to be 0.0639,0.0658,and 0.0673 mg·mL-1 while the contents of total alkaloids in serum of Complete Frederian adjuvantinduced prostate inflammation model rats were estimated to be 0.0546,0.0569,and0.0594 mg·mL-1.The results proved that the total alkaloids of the Mongolian medicine compound Xieriga-4 could adjust the non-bacterial prostatitis.It provides a scientific basis for the treatment of non-bacterial prostatitis with Mongolian medicine compound Xieriga-4.
The normal group and complete Frederian adjuvant-induced prostatein flammation model group rats were gavaged with the same dose of Mongolian medicine compound Xieriga-4 extract.The absorbance of total terpene in rat serum at 419 nm wavelength was monitored using UVvis spectrophotometer with the berberine as the standard.The contents of total alkaloids in the serum of normal and complete Frederian adjuvant-induced prostate inflammation model rats gavaged with Mongolian medicine compound Xieriga-4 were calculated.The results showed that the contents of total alkaloids in the serum of three batches of normal rats were determined to be 0.0639,0.0658,and 0.0673 mg·mL-1 while the contents of total alkaloids in serum of Complete Frederian adjuvantinduced prostate inflammation model rats were estimated to be 0.0546,0.0569,and0.0594 mg·mL-1.The results proved that the total alkaloids of the Mongolian medicine compound Xieriga-4 could adjust the non-bacterial prostatitis.It provides a scientific basis for the treatment of non-bacterial prostatitis with Mongolian medicine compound Xieriga-4.
引文
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[20] Fukuda K,Hibiya Y,Mutoh M,et al.Inhibition by berberine of cyclooxygenase-2transcriptional activity in human colon cancer cells[J].Journal of Ethnopharmacology,1999,66(2):227-233.
[2]阿拉腾其木格,金亮.蒙药协日嘎-4味汤散的研究进展[J].北方药学,2016,13(1):94-96.
[3]高建华,李斌鑫,白凤,等.蒙药复方协日嘎四味化学成分的研究[J].北京中医药大学学报,2016,39(9):755-758.
[4]陈阳峰,钟晓红.黄柏的药理作用及其活性成分提取[J].作物研究,2015,29(5):564-568.
[5]王季.黄柏有效部位的化学成分的研究[J].黑龙江医药,2014,27(2):292-296.
[6]廉莲,咸晓燕,楚冬海,等.川黄柏的化学成分研究[J].中国实验方剂学杂志,2013,19(19):149-152.
[7]李春娜,范冰舵,刘洋洋,等.蒺藜茎叶的化学成分研究[J].中华中医药杂志,2015,30(9):3294-3297.
[8]赵百岁,宝音图,田吉.蒙药材蒺藜的化学成分研究进展[J].中国民族医药杂志,2008,14(11):73-76.
[9]杨婷.蒙药复方协日嘎-4有效物质基础研究[D].呼和浩特:内蒙古医科大学,2013.
[10]周莉,王永,骆永伟,等.免疫性非细菌性前列腺炎大鼠内环境因素关联性研究[J].中国临床药理学与治疗学,2014,19(9):995-1000.
[11]孙继红,胡梅,巫凤娟,等.花川保列颗粒对大鼠非菌性前列腺炎模型的实验研究[J].中国实验方剂学杂志,2010,16(4):135-138.
[12]杨小荣,刘芳,谢文杰,等.大鼠非细菌性前列腺炎模型的复制[J].实验与检验医学,2010,28(4):347-348.
[13] Xu Y,Ventura S.Extracts of bark from the traditional Chinese herb Phellodendron amurense,inhibit contractility of the isolated rat prostate gland[J].Journal of Ethnopharmacology,2010,127(1):196-199.
[14]陈伟中.刺蒺藜治疗前列腺炎作用的探讨[J].新中医,2012,44(7):201-203.
[15]孙森凤,张颖颖,褚万春.黄柏药理作用的研究进展[J].山东化工,2017,46(14):99-100.
[16] Park E K,Rhee H I,Jung H S,et al.Antiinflammatory effects of a combined herbal preparation(RAH13)of Phellodendron amurense and Coptis chinensis in animal models of inflammation[J].Phytotherapy Research Ptr,2010,21(8):746-750.
[17]李丹丹,江培,杨书美,等.黄柏的化学成分、药理作用及临床应用研究进展[J].黑龙江医药,2014,27(3):601-605.
[18]周松,刘永刚,张国祥,等.黄柏化学成分及质量控制研究进展[J].中国药房,2012,23(39):3740-3742.
[19]张冠英,董瑞娟,廉莲.川黄柏、关黄柏的化学成分及药理活性研究进展[J].沈阳药科大学学报,2012,29(10):812-821.
[20] Fukuda K,Hibiya Y,Mutoh M,et al.Inhibition by berberine of cyclooxygenase-2transcriptional activity in human colon cancer cells[J].Journal of Ethnopharmacology,1999,66(2):227-233.