反向毛细管电泳-激光诱导荧光快速分析面粉中的荧光增白剂
|
摘要
建立了反向毛细管电泳-激光诱导荧光法同时分析面粉中的荧光增白剂VBL和荧光增白剂FBA351的方法。探讨了电泳介质的种类、浓度、添加剂、pH值及分离电压等因素。在分离电压为-19.0 kV,激发波长为405 nm,发射波长为430 nm,缓冲液为90 mmol·L~(-1) Na_2B_4O_7-0.2mmol·L~(-1) CTAB-15%乙腈(pH=9.2)的条件下,可在7 min内实现两种荧光染料的分离检测。线性范围均为120.0~950.0 ng·mL~(-1),检出限分别为55.4和67.5 ng·mL~(-1),回收率分别为103.4%和97.3%。可用于面粉中两种荧光染料的分离检测。
A method was established for the simultaneous separation and determination of fluorescent brightener VBL and fluorescent brightener FBA in flour by reversed capillary electrophoresis with laser induced fluorescence detection. The influence factors of the tape, concentration, additive and pH of buffer, the separation voltage on the separation and detection were researched and the best analysis conditions were obtained. In this study, fluorescent brightener VBL and fluorescent brightener FBA 351 were separated and detected within 7 min, in running medium of 90 mmol·L~(-1) Na_2B_4O_7-0.2 mmol·L~(-1) CTAB-15% acetonitrile(pH= 9.2) at the separation voltage of-19.0 kV with the detection wavelength was set as 405/430 nm(λex/λem). Linearity ranges of both fluorescent brightener VBL and fluorescent brightener FBA 351 were found between 120.0 ~ 950.0 ng·mL~(-1), the limit of detection(S/N = 3) of the fluorescent brightener were found to be 55.4 and 67.5 ng·mL~(-1). The recoveries were 103.4% and 97.3%, respectively. The method could be used for the determination of the two fluorescent brightener in flour samples.
A method was established for the simultaneous separation and determination of fluorescent brightener VBL and fluorescent brightener FBA in flour by reversed capillary electrophoresis with laser induced fluorescence detection. The influence factors of the tape, concentration, additive and pH of buffer, the separation voltage on the separation and detection were researched and the best analysis conditions were obtained. In this study, fluorescent brightener VBL and fluorescent brightener FBA 351 were separated and detected within 7 min, in running medium of 90 mmol·L~(-1) Na_2B_4O_7-0.2 mmol·L~(-1) CTAB-15% acetonitrile(pH= 9.2) at the separation voltage of-19.0 kV with the detection wavelength was set as 405/430 nm(λex/λem). Linearity ranges of both fluorescent brightener VBL and fluorescent brightener FBA 351 were found between 120.0 ~ 950.0 ng·mL~(-1), the limit of detection(S/N = 3) of the fluorescent brightener were found to be 55.4 and 67.5 ng·mL~(-1). The recoveries were 103.4% and 97.3%, respectively. The method could be used for the determination of the two fluorescent brightener in flour samples.
引文
[1]熊培芳.中国食品安全问题现状成因及对策研究[J].农业与技术,2016,36(7):175-176.
[2]蒋治国,吴冬梅,堵燕钰,等.食用菌中荧光增白剂的风险评估[J].环境与可持续发展,2010,35(5):47-49.
[3]陈礼英.隐藏的白色杀手——荧光增白剂[J].化工管理,2014(19):92-97.
[4]Li J M,Zhai H Y,Chen Z G,et al.Preparation and evalution of a novel molecularly imprinted SPE monolithic capillary column for the determination of auramine O in shrimp[J].J Sep Sci,2013,36(21-22):3608-3614.
[5]欧盟严格限制食品中禁用染料含量[J].食品工业科技,2005(8):192.
[6]程春梅,彭进,董刘敏.高效液相色谱法同时检测果汁饮料中的六种合成着色剂[J].食品安全与检测,2011,36(10):301-304.
[7]伊雄海,邓晓军,杨慧琴,等.液相色谱-串联质谱法检测食品中的多种易滥用着色剂[J].色谱,2011,29(11):1062-1069.
[8]高文惠,裴红,杨贵君.毛细管电泳在食品添加剂检测中的应用[J].食品与生物技术学报,2010,29(3):326-330.
[9]吕双,蒲爱军.荧光增白剂VBL的高效液相色谱法定量分析[J].染料与染色,2015,52(4):36-37.
[10]田益玲,陈冠华,祝彦忠.高效毛细管电泳在食品添加剂中应用的研究进展[J].河北农业大学学报,2002,25(5):227-229.
[11]郭东山,陈冠华,董明珠,等.毛细管电泳-量子点间接激光诱导荧光检测5钟食品防腐剂[J].分析化学,2012,40(9):1379-1384.
[12]洪晓明,刘斌,张世伟,等.毛细管-激光诱导荧光免疫分析在食品安全领域的应用与研究展望[J].安徽农业科学,2013,41(8):3644-3646.
[13]余长柱.毛细管电泳激光诱导荧光检测方法及应用研究[D].安徽,中国科学技术大学,2008.
[14]王立卫,闫正,邢超,等.高效毛细管电泳法测定葡萄糖中苋菜红及亮蓝[J].化学分析计量,2013,22(6):43-45.
[15]赵新颖,贾丽,周晓晶,等.毛细管电泳法同时测定糖果中5种人工合成色素的含量[J].现代仪器,2008,14(4):58-60.
[16]杨桂君,高文惠.毛细管电泳法测定食品中8中添加剂[J].食品科学,2010,31(18):377-380.
[17]Kuo T Y,Wang S P,Lin C H.Online identification of the fluorescent whitening agent 4,4-bis(2-sulfostyrol)biphenyl using a sweeping technique combined with capillary electrophoresis/77 K fluorescence spectroscopy[J].Electrophoresis,2005,26(12):4355-4359.
[18]Zhai H Y,Yuan K S,Yu X,et al.A simple and compact fluorescence detection system for capillary electrophoresis and its application to food analysis[J].Electrophoresis,2015,36(20):2509-2519.
[2]蒋治国,吴冬梅,堵燕钰,等.食用菌中荧光增白剂的风险评估[J].环境与可持续发展,2010,35(5):47-49.
[3]陈礼英.隐藏的白色杀手——荧光增白剂[J].化工管理,2014(19):92-97.
[4]Li J M,Zhai H Y,Chen Z G,et al.Preparation and evalution of a novel molecularly imprinted SPE monolithic capillary column for the determination of auramine O in shrimp[J].J Sep Sci,2013,36(21-22):3608-3614.
[5]欧盟严格限制食品中禁用染料含量[J].食品工业科技,2005(8):192.
[6]程春梅,彭进,董刘敏.高效液相色谱法同时检测果汁饮料中的六种合成着色剂[J].食品安全与检测,2011,36(10):301-304.
[7]伊雄海,邓晓军,杨慧琴,等.液相色谱-串联质谱法检测食品中的多种易滥用着色剂[J].色谱,2011,29(11):1062-1069.
[8]高文惠,裴红,杨贵君.毛细管电泳在食品添加剂检测中的应用[J].食品与生物技术学报,2010,29(3):326-330.
[9]吕双,蒲爱军.荧光增白剂VBL的高效液相色谱法定量分析[J].染料与染色,2015,52(4):36-37.
[10]田益玲,陈冠华,祝彦忠.高效毛细管电泳在食品添加剂中应用的研究进展[J].河北农业大学学报,2002,25(5):227-229.
[11]郭东山,陈冠华,董明珠,等.毛细管电泳-量子点间接激光诱导荧光检测5钟食品防腐剂[J].分析化学,2012,40(9):1379-1384.
[12]洪晓明,刘斌,张世伟,等.毛细管-激光诱导荧光免疫分析在食品安全领域的应用与研究展望[J].安徽农业科学,2013,41(8):3644-3646.
[13]余长柱.毛细管电泳激光诱导荧光检测方法及应用研究[D].安徽,中国科学技术大学,2008.
[14]王立卫,闫正,邢超,等.高效毛细管电泳法测定葡萄糖中苋菜红及亮蓝[J].化学分析计量,2013,22(6):43-45.
[15]赵新颖,贾丽,周晓晶,等.毛细管电泳法同时测定糖果中5种人工合成色素的含量[J].现代仪器,2008,14(4):58-60.
[16]杨桂君,高文惠.毛细管电泳法测定食品中8中添加剂[J].食品科学,2010,31(18):377-380.
[17]Kuo T Y,Wang S P,Lin C H.Online identification of the fluorescent whitening agent 4,4-bis(2-sulfostyrol)biphenyl using a sweeping technique combined with capillary electrophoresis/77 K fluorescence spectroscopy[J].Electrophoresis,2005,26(12):4355-4359.
[18]Zhai H Y,Yuan K S,Yu X,et al.A simple and compact fluorescence detection system for capillary electrophoresis and its application to food analysis[J].Electrophoresis,2015,36(20):2509-2519.