高效液相色谱法测定3种剂型黄芪中黄芪甲苷和毛蕊异黄酮葡萄糖苷含量
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摘要
目的比较3种剂型黄芪中有效成分的含量。方法采用高效液相色谱蒸发光散射检测法测定黄芪甲苷含量,采用高效液相色谱紫外光检测法测定毛蕊异黄酮葡萄糖苷含量。结果黄芪饮片、黄芪配方颗粒、黄芪最细粉中黄芪甲苷的平均含量分别为(0. 074 6±0. 003 5)%,(0. 056±0. 004 5)%,(0. 129±0. 005 1)%,毛蕊异黄酮葡萄糖苷的平均含量分别为(0. 031±0. 002 1)%,(0. 022±0. 002 4)%,(0. 025±0. 001 9)%。结论 3种剂型黄芪中黄芪甲苷和毛蕊异黄酮葡萄糖苷含量均达到2015年版《中国药典(一部)》规定标准。黄芪最细粉中黄芪甲苷的含量最高,黄芪饮片中毛蕊异黄酮葡萄糖苷含量最高。本研究中建立的方法操作简便,可用于3种黄芪剂型中有效成分的含量测定。
Objective To compare the contents of active ingredients in three dosage forms of Astragalus membranaceus. Methods The content of astragaloside Ⅳ was determined by HPLC-ELSD method,and the content of calycosin glycoside was determined by HPLC-UV method. Results The average content of astragaloside Ⅳ in decoction pieces of Astragalus membranaceus, dispensing granules of Astragalus membranaceus and the finest powder of Astragalus membranaceus was( 0. 074 6 ± 0. 003 5) %,( 0. 056 ± 0. 004 5) % and( 0. 129 ± 0. 005 1) %. The average content of calycosin glycoside in decoction pieces of Astragalus membranaceus,dispensing granules of Astragalus membranaceus and the finest powder of Astragalus membranaceus was( 0. 031 ± 0. 002 1) %,( 0. 022 ± 0. 002 4) % and( 0. 025 ± 0. 001 9) %. Conclusion The content of astragaloside Ⅳ and calycosin glycoside in three dosage forms of Astragalus membranaceus meets the standard of Chinese Pharmacopoeia( Volume Ⅰ,2015 edition). The content of astragaloside Ⅳ in the finest powder of Astragalus membranaceus is the highest,and the content of calycosin glycoside in the decoction pieces of Astragalus membranaceus is the highest. The established method is simple and can be used for the content determination of active ingredients in three dosage forms of Astragalus preparations.
Objective To compare the contents of active ingredients in three dosage forms of Astragalus membranaceus. Methods The content of astragaloside Ⅳ was determined by HPLC-ELSD method,and the content of calycosin glycoside was determined by HPLC-UV method. Results The average content of astragaloside Ⅳ in decoction pieces of Astragalus membranaceus, dispensing granules of Astragalus membranaceus and the finest powder of Astragalus membranaceus was( 0. 074 6 ± 0. 003 5) %,( 0. 056 ± 0. 004 5) % and( 0. 129 ± 0. 005 1) %. The average content of calycosin glycoside in decoction pieces of Astragalus membranaceus,dispensing granules of Astragalus membranaceus and the finest powder of Astragalus membranaceus was( 0. 031 ± 0. 002 1) %,( 0. 022 ± 0. 002 4) % and( 0. 025 ± 0. 001 9) %. Conclusion The content of astragaloside Ⅳ and calycosin glycoside in three dosage forms of Astragalus membranaceus meets the standard of Chinese Pharmacopoeia( Volume Ⅰ,2015 edition). The content of astragaloside Ⅳ in the finest powder of Astragalus membranaceus is the highest,and the content of calycosin glycoside in the decoction pieces of Astragalus membranaceus is the highest. The established method is simple and can be used for the content determination of active ingredients in three dosage forms of Astragalus preparations.
引文
[1]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:302-303.
[2]贺爱玲.中药配方颗粒临床应用现状分析及展望[J].中国药事,2017,31(10):1205-1209.
[3]潘昆,金浪.我国中药配方颗粒存在的质量问题及其监管体系探究[J].卫生软科学,2017,31(9):46-48.
[4]刘芳,傅超美,李小红,等.中药饮片的研究与应用进展分析[J].中国实验方剂学杂志,2016,22(2):222-225.
[5]张火旺,蔡勇科,吴志国,等.高效液相色谱-蒸发光散射检测法测定养阴生津片中黄芪甲苷含量[J].中国药业,2016,25(20):53-55.
[6]宋成英,封加福. HPLC同时测定黄芪药材中毛蕊异黄酮葡萄糖苷和黄芪甲苷[J].中国实验方剂学杂志,2013,19(11):115-117.
[7]刘志勤.三种中药饮片的比较[J].中外医学研究,2011,9(10):96.
[8]邓雯,杨泽锐,程翔燕,等.不同粒径黄芪超微细粉理化性质的研究[J].中国现代中药,2017,19(8):1187-1192.
[9]窦霞,靳子明,甄小龙.党参超微粉与细粉在大鼠体内的药动学比较研究[J].中国医院药学杂志,2016,36(20):1749-1752.
[10]钟瑞坤.中药配方颗粒优劣浅析[J].北方药学,2011,8(6):73-74.
[11]李亚玲,曾超,叶云,等.中药传统饮片与中药配方颗粒治疗老年功能性便秘的临床疗效[J].中国医院药学杂志,2017,37(23):2378-2382.
[12]王晓琳,张平.黄芪饮片与黄芪配方颗粒有效成分含量比较[J].西部中医药,2017,30(2):25-27.
[13]俞路宁,徐筱芳,邵深深,等.传统煎法中药与配方颗粒比较[J].长春中医药大学学报,2015,26(5):784-785.
[14]范羽丰,谭蓉,胡晓波.黄连饮片极细粉化形式的考察[J].抗感染药学,2007,4(3):114-115.
[15]四川省食品药品监督管理局.四川省中药饮片炮制规范[M].成都:四川科学技术出版社,2015:199-201.
[2]贺爱玲.中药配方颗粒临床应用现状分析及展望[J].中国药事,2017,31(10):1205-1209.
[3]潘昆,金浪.我国中药配方颗粒存在的质量问题及其监管体系探究[J].卫生软科学,2017,31(9):46-48.
[4]刘芳,傅超美,李小红,等.中药饮片的研究与应用进展分析[J].中国实验方剂学杂志,2016,22(2):222-225.
[5]张火旺,蔡勇科,吴志国,等.高效液相色谱-蒸发光散射检测法测定养阴生津片中黄芪甲苷含量[J].中国药业,2016,25(20):53-55.
[6]宋成英,封加福. HPLC同时测定黄芪药材中毛蕊异黄酮葡萄糖苷和黄芪甲苷[J].中国实验方剂学杂志,2013,19(11):115-117.
[7]刘志勤.三种中药饮片的比较[J].中外医学研究,2011,9(10):96.
[8]邓雯,杨泽锐,程翔燕,等.不同粒径黄芪超微细粉理化性质的研究[J].中国现代中药,2017,19(8):1187-1192.
[9]窦霞,靳子明,甄小龙.党参超微粉与细粉在大鼠体内的药动学比较研究[J].中国医院药学杂志,2016,36(20):1749-1752.
[10]钟瑞坤.中药配方颗粒优劣浅析[J].北方药学,2011,8(6):73-74.
[11]李亚玲,曾超,叶云,等.中药传统饮片与中药配方颗粒治疗老年功能性便秘的临床疗效[J].中国医院药学杂志,2017,37(23):2378-2382.
[12]王晓琳,张平.黄芪饮片与黄芪配方颗粒有效成分含量比较[J].西部中医药,2017,30(2):25-27.
[13]俞路宁,徐筱芳,邵深深,等.传统煎法中药与配方颗粒比较[J].长春中医药大学学报,2015,26(5):784-785.
[14]范羽丰,谭蓉,胡晓波.黄连饮片极细粉化形式的考察[J].抗感染药学,2007,4(3):114-115.
[15]四川省食品药品监督管理局.四川省中药饮片炮制规范[M].成都:四川科学技术出版社,2015:199-201.