笔管草质量标准研究
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摘要
目的制定笔管草的质量标准,提供其质量控制的依据。方法观察笔管草药材性状和显微鉴别特征,以笔管草对照药材进行笔管草的薄层色谱鉴别。采用高效液相色谱(HPLC)法测定药材中山柰酚的含量。色谱柱为Thermo BETASIL C18;流动相为乙腈-0.4%磷酸溶液(32∶68,V/V);检测波长为365 nm;柱温为30℃;进样量10μL。结果笔管草的性状和显微鉴别特征显著;薄层色谱斑点清晰,分离良好; HPLC法测定笔管草中山柰酚的含量在1.22～381.00μg·m L~(-1)的范围内呈良好的线性关系(Y=40.178 2X+27.948 4,r=0.999 9),测得5批笔管草的山柰酚含量平均值为1.5 mg·g~(-1),加样回收率平均值为94.6%,RSD=3.12%(n=6)。结论所建立的质量标准控制方法简便准确,重现性好,可用于笔管草药材的质量控制。
OBJECTIVE To establish the quality standard for the Equisetum ramosissimum subsp. debile and provide the basis for its quality control. METHODS The characteristics and microscopic identification of the medicinal herbs were observed. The TLC identification of the herbs was compared with the reference crude herb. Herbs were determined by HPLC method in kaempferiagalanga phenol content: Chromatographic column for Thermo BETASIL C18.Mobile phase of acetonitrile-0.4% phosphoric acid solution( 32 ∶68,V/V).Detection wavelength of 365 nm.The column temperature was 30 ℃.Sample quantity 10 μL. RESULTS The characteristics and microscopic identification of the herbs were remarkable. Thin layer chromatographic spots were clear,separation was good.The Equisetum ramosissimum subsp. debilewas determined by HPLC method in kaempferiagalanga phenol,A good linear relation-ship was obtained within the range of 1.22-381. 00 μg·m L~(-1)( Y = 40.178 2 X+ 27.948 4,r = 0.999 9).The average value of kaempferiagalanga phenol content could be measured in the 5 batchs of Equisetum ramosissimum subsp. debile was 1.5 mg·g~(-1),the average value of the adding standard recoveries was 94.6%,RSD = 3.12%( n = 6). CONCLUSION The quality standard control method is simple and accurate,reproducible,and can be used as the quality control of the Equisetum ramosissimum subsp. debile.
OBJECTIVE To establish the quality standard for the Equisetum ramosissimum subsp. debile and provide the basis for its quality control. METHODS The characteristics and microscopic identification of the medicinal herbs were observed. The TLC identification of the herbs was compared with the reference crude herb. Herbs were determined by HPLC method in kaempferiagalanga phenol content: Chromatographic column for Thermo BETASIL C18.Mobile phase of acetonitrile-0.4% phosphoric acid solution( 32 ∶68,V/V).Detection wavelength of 365 nm.The column temperature was 30 ℃.Sample quantity 10 μL. RESULTS The characteristics and microscopic identification of the herbs were remarkable. Thin layer chromatographic spots were clear,separation was good.The Equisetum ramosissimum subsp. debilewas determined by HPLC method in kaempferiagalanga phenol,A good linear relation-ship was obtained within the range of 1.22-381. 00 μg·m L~(-1)( Y = 40.178 2 X+ 27.948 4,r = 0.999 9).The average value of kaempferiagalanga phenol content could be measured in the 5 batchs of Equisetum ramosissimum subsp. debile was 1.5 mg·g~(-1),the average value of the adding standard recoveries was 94.6%,RSD = 3.12%( n = 6). CONCLUSION The quality standard control method is simple and accurate,reproducible,and can be used as the quality control of the Equisetum ramosissimum subsp. debile.
引文
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[19]隋长惠,张皛,商凤春,等.木贼及其混淆品的鉴别[J].沈阳药科大学学报,1996(4):39-44.
[2]吴国土,薛玲,黄自强.笔管草醇提物的调节血脂作用[J].齐齐哈尔医学院报,2004(2):121-123.
[3]吴国土,薛玲,黄自强.笔管草水提物对大鼠及高脂家兔的降脂作用[J].福建医科大学学报,2004(1):59-63.
[4]陈志奎.笔管草活性成分ED-I调血脂作用及机制研究[D].福州:福建医科大学,2005.
[5]Awan A B. A pharmacological evidence for the presence of antihistaminic and anticholinergic activities in Equisetum debile Roxb[J].Indian J Pharmacol,2017,49(1):98-101.
[6]周荣汉,于荣敏.木贼科植物化学成分研究概况[J].中药通报,1985,10(3):5-9.
[7]佟丽华,赵艳,王晓丹.不同产地木贼有效成分的研究[J].黑龙江中医药,1992,1(5):43-44.
[8]周荣汉,于荣敏.木贼科植物化学成分的研究概况[J].沈阳药学院学报,1984(1):72-80.
[9]许小红,阮宝强,蒋山好,等.笔管草中Megastigmane及黄酮苷类化学成分[J].中国天然药物,2005,3(2):93-96.
[10]梁晓华,徐成东,王陆琴.云南13种蕨类植物中总黄酮的测定[J].安徽农业科学,2008,36(19):7965-7966.
[11]黄有霖.福建省中药材标准[M].福州:海风出版社,2006:211-212.
[12]中国科学院中国植物志编辑委员会.中国植物志(第6卷第3分册)[M].北京:科学出版社,2004:236-238.
[13]隋长惠,王玉洁,张建超,等.木贼混淆品———笔管草的显微鉴定[J].现代应用药学,1997(2):12-13.
[14]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015:57.
[15]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015:1502,377.
[16]刘华钢,梁美艳,宁带连,等.壮药笔管草的质量标准研究[J].广西中医药,2013,36(2):57-59.
[17]刘振启,刘杰.木贼与混乱品的鉴别[J].首都食品与医药,2015,22(13):55.
[18]于荣敏,李铣,周荣汉.中国木贼科的形态组织学与分类学研究[J].沈阳药学院学报,1986(4):279-284.
[19]隋长惠,张皛,商凤春,等.木贼及其混淆品的鉴别[J].沈阳药科大学学报,1996(4):39-44.