复方黄连灌肠液的总黄酮测定方法研究
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摘要
目的建立复方黄连灌肠液中总黄酮含量测定方法。方法采用紫外-可见分光光度法测定。比较直接测定、AlCl_3-CH_3COONa显色体系、NaNO_2-Al(NO_3)_3-NaOH显色体系等3种不同显色方法,分析以槲皮素、芦丁、橙皮苷为对照品的波长扫描图谱,探讨最佳测定条件。结果最佳显色方案为采用NaNO_2-Al(NO_3)_3-NaOH显色法,以芦丁为对照品,在371 nm波长下进行测定;芦丁在2.488～12.44μg/ml范围内线性关系良好(r=0.9995),加样回收率平均值为100.43%(RSD=1.33%)。结论 NaNO_2-Al(NO_3)_3-NaOH显色法适用于复方黄连灌肠液总黄酮的含量测定,该法简便、快速、稳定可靠。
Objective To establish a method for the determination of the content of total flavonoids in compound Huanglian clysis.Methods Three reference substances and the three coloration methods were studied to find the ideal coloration method and determination conditions by spectrophotometry.Results The sample was detected at 371 nm wavelength by NaNO_2-Al(NO_3)_3-NaOH reaction with rutin as reference substance.A good linear relationship was observed in the concentration range of 2.488～12.44μg/ml for querceetin(r=0.9993),and the average recovery rate was 100.43% with RSD of 1.33%.Conclusion The NaNO_2-Al(NO_3)_3-NaOH reaction was simple,quick,stable and reliable,which was proved to be applicable for the determination of the content of total flavonoids in compound Huanglian clysis.
Objective To establish a method for the determination of the content of total flavonoids in compound Huanglian clysis.Methods Three reference substances and the three coloration methods were studied to find the ideal coloration method and determination conditions by spectrophotometry.Results The sample was detected at 371 nm wavelength by NaNO_2-Al(NO_3)_3-NaOH reaction with rutin as reference substance.A good linear relationship was observed in the concentration range of 2.488～12.44μg/ml for querceetin(r=0.9993),and the average recovery rate was 100.43% with RSD of 1.33%.Conclusion The NaNO_2-Al(NO_3)_3-NaOH reaction was simple,quick,stable and reliable,which was proved to be applicable for the determination of the content of total flavonoids in compound Huanglian clysis.
引文
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[2] 孙莲,阿合买提江,马晓丽,等.HPLC法同时测定新疆药桑叶中芦丁、异槲皮苷、槲皮素与绿原酸的含量[J].中国药房,2011,22(23):2177-2179.
[3] 范世明,谢心月,曾繁天,等.三叶青叶化学成分鉴定及其总黄酮含量测定研究[J].药物分析杂志,2017,37(8):1481-1488.
[4] 杨志欣,王海威,张文君.苦参总黄酮含量测定方法的优化[J].中成药,2017,39(5):946-951.
[5] 赵美,曾亚,周晓英.阿尔泰金莲花中总黄酮含量测定的方法比较研究[J].新疆医科大学学报,2017,40(10):1342-1345.
[6] 李艳杰,鲁文慧,罗伙军.草果配方颗粒中总黄酮含量测定[J].吉林中医药,2017,37(5):517-519.
[7] 江冬英,金建辉,袁海燕.常舒茶中总黄酮的测定方法[J].数理医药学杂志,2015,28(11):1674-1676.
[8] 韦瑀龙,黄小鸥,蓝晓庆.固本补肾口服液的总黄酮含量测定方法研究[J].实用药物与临床,2016,19(10):1287-1289.
[9] 宋琳琳,胡晓艳,胡晓冬,等.双参口服液中总黄酮和总皂苷的含量测定[J].黑龙江医药科学,2017,40(6):11-13.
[10] 刘基,赵宁,王小双,等.几种云南核桃不同部位总黄酮含量的测定[J].山东农业科学,2017,49(12):135-138.
[11] 热孜亚·吾甫尔,艾尼瓦尔·塔力甫,买迪娜木·米吉提,等.紫外分光光度法测定维吾尔药复方艾皮提蒙合剂中总黄酮含量[J].中国民族医药杂志,2017,8(8):44-45.
[12] 常飞,吴文能,曹晖.白补药总黄酮含量测定方法的建立[J].天然产物研究与开发,2016,(28):71-75.