灵芝孢子粉对小鼠抗疲劳作用及生化机制初探
|
摘要
目的观察灵芝孢子粉对小鼠的抗疲劳作用并探讨其生化机制。方法 80只SPF级ICR小白鼠,按体质量随机分为对照组和灵芝孢子粉高剂量(500mg/kg)、中剂量(300mg/kg)、低剂量(100mg/kg)实验组,每组20只,雌雄各半。根据小鼠体质量和实验设计的剂量,每只小鼠按0.2 ml/10g的灌胃量于每天上午灌胃灵芝孢子粉溶液一次。实验周期为30d。末次灌胃30 min后,小鼠在30℃的温水浴中进行负重游泳实验;剖杀后采集小鼠肝脏和后腿肌肉组织,检测肝糖原和肌糖原含量,同时采集小鼠血清,检测血清中葡萄糖、尿素氮含量和乳酸脱氢酶(lactate dehydrogenase, LDH)、和碱性磷酸酶(alkaline phosphatase, AKP)等酶活性。结果灌胃不同剂量灵芝孢子粉30d后,各组小鼠的游泳时间明显长于对照组,高剂量组雌、雄鼠差异显著(5.53±1.44、16.90±5.98和10.65±1.26、20.61±3.31),分别提高了205.6%和93.5%;与对照组相比,三个剂量组小鼠肝糖原含量均显著升高(P<0.01);雌性小鼠肌糖原含量较对照组略有降低,雄性小鼠肌糖原含量较对照组略有升高,但各组间差异均不显著(P>0.05)。各剂量组小鼠血糖含量都高于对照组,且对照组与高剂量组差异显著(1.43±0.03、2.94±0.11,1.78±0.04、2.75±0.07,P<0.05);各剂量组小鼠血清尿素氮含量低于对照组。灌胃灵芝孢子粉各剂量组雌鼠血清中AKP活性均低于对照组,且在高剂量组显著或极显著降低(P<0.05,P<0.01)。与对照组相比,各剂量组小鼠血清LDH活性显著或极显著升高(P<0.05、P<0.01)。结论灌胃灵芝孢子粉可以延长小鼠游泳时间,提高小鼠抗疲劳能力。其生化特点表现在:一方面通过增加小鼠肝脏和肌肉中糖原的积累,提高了小鼠的能量储备;另一方面通过减缓尿素氮的累积和血糖水平的下降,使机体对负荷的适应性增强。[营养学报,2019,41(2):173-177]
Objective To observe the anti-fatigue effect of Ganoderma lucidum spore powder on mice and explore its biochemical mechanism. Methods Eighty SPF-class ICR mice were randomly divided into control group and Ganoderma lucidum spore powder high dose(500 mg/kg), medium dose(300 mg/kg), low dose(100 mg/kg) groups with 20 mice in each group, half male and half female. According to the body weight and the experimentally designed dose, each mouse was given to Ganoderma lucidum spore powder solutions once a day at a dose of 0.2 ml/10 g by intragastric administration for 30 days.After 30 minutes of the last gavage, the time of weight-bearing swimming in mice were tested in a warm water bath at 30℃.And the liver and hind leg muscles were collected and the contents of hepatic and muscle glycogen were measured.Furthermore, the serum was collected and the contents of glucose and urea nitrogen and the related enzyme activities were measured, such as lactate dehydrogenase and alkaline phosphatase. Results After 30 days of different treatments the swimming time of experimental groups was significantly longer than that of the control group,(5.53±1.44,16.90±5.98 and10.65±1.26,20.61±3.31, P<0.01), and increased by 205.6% and 93.5% respectively. The content of hepatic glycogen in three dose groups was significantly higher than that in control group(P<0.01). However, the content of muscle glycogen in male mice was slightly higher than that in control group, but there was no significant difference between different groups(P>0.05).Meanwhile, after the test of weight-bearing swimming, the blood glucose levels of the mice, fed with different doses of Ganoderma lucidum spore powders solution, were higher than those of the control group(1.43±0.03,2.94±0.11,1.78±0.04,2.75±0.07,P<0.05). In addition, the content of blood urea nitrogen was lower than that in the control group. The activities of AKP in the serum of the mice treated with Ganoderma lucidum spore powders were lower than those in the control group,and significantly decreased(P<0.05, P<0.01) in the high dose group. The serum LDH's activity of experimental groups was significantly higher than that of the control group(P<0.05, P<0.01). Conclusion The results showed that Ganoderma lucidum spore powders could prolong the swimming time and improve the anti-fatigue ability of mice. Ganoderma lucidum spore powders could improve energy reserve by increasing the mouse liver and muscle glycogen accumulation, and reduce the accumulation of urea nitrogen. [ACTA NUTRIMENTA SINICA, 2019, 41(2):173-177]
Objective To observe the anti-fatigue effect of Ganoderma lucidum spore powder on mice and explore its biochemical mechanism. Methods Eighty SPF-class ICR mice were randomly divided into control group and Ganoderma lucidum spore powder high dose(500 mg/kg), medium dose(300 mg/kg), low dose(100 mg/kg) groups with 20 mice in each group, half male and half female. According to the body weight and the experimentally designed dose, each mouse was given to Ganoderma lucidum spore powder solutions once a day at a dose of 0.2 ml/10 g by intragastric administration for 30 days.After 30 minutes of the last gavage, the time of weight-bearing swimming in mice were tested in a warm water bath at 30℃.And the liver and hind leg muscles were collected and the contents of hepatic and muscle glycogen were measured.Furthermore, the serum was collected and the contents of glucose and urea nitrogen and the related enzyme activities were measured, such as lactate dehydrogenase and alkaline phosphatase. Results After 30 days of different treatments the swimming time of experimental groups was significantly longer than that of the control group,(5.53±1.44,16.90±5.98 and10.65±1.26,20.61±3.31, P<0.01), and increased by 205.6% and 93.5% respectively. The content of hepatic glycogen in three dose groups was significantly higher than that in control group(P<0.01). However, the content of muscle glycogen in male mice was slightly higher than that in control group, but there was no significant difference between different groups(P>0.05).Meanwhile, after the test of weight-bearing swimming, the blood glucose levels of the mice, fed with different doses of Ganoderma lucidum spore powders solution, were higher than those of the control group(1.43±0.03,2.94±0.11,1.78±0.04,2.75±0.07,P<0.05). In addition, the content of blood urea nitrogen was lower than that in the control group. The activities of AKP in the serum of the mice treated with Ganoderma lucidum spore powders were lower than those in the control group,and significantly decreased(P<0.05, P<0.01) in the high dose group. The serum LDH's activity of experimental groups was significantly higher than that of the control group(P<0.05, P<0.01). Conclusion The results showed that Ganoderma lucidum spore powders could prolong the swimming time and improve the anti-fatigue ability of mice. Ganoderma lucidum spore powders could improve energy reserve by increasing the mouse liver and muscle glycogen accumulation, and reduce the accumulation of urea nitrogen. [ACTA NUTRIMENTA SINICA, 2019, 41(2):173-177]
引文
[1]林楠,钟琳琳,李娜,等.人参花提取物抗疲劳作用的研究[J].长春中医药大学学报,2010,26:336-337.
[2]麻懿馨,范文今,赵翠霞,等.抗疲劳中药对小鼠抗疲劳作用的实验研究[J].上海预防医学,2013,25:592-594.
[3]夏勇,赵硕,傅剑云.灵芝粉抗疲劳作用的实验研究[J].中国临床康复,2002,6:3618-3619.
[4]覃筱燕,唐丽,杨林,等.枸杞提取物对小鼠抗疲劳作用的实验研究[J].中医药学报,2009,37:8-11.
[5]关力.大脑疲劳的研究与进展[J].国外医学生物医学工程分册,1996,19:9-13.
[6]何来英,严卫星,楼密密,等.保健食品抗疲劳作用实验方法研究[J].中国食品卫生杂志,1997,9:1-7
[7]杨国宇,王艳玲,王月影,等.肌肽对小鼠抗疲劳作用的实验研究[J].河南农业大学学报,2003,37:383-385.
[8]Allen DG,Lamb GD,Westerblad H.Skeletal muscle fatigue:cellular mechanisms[J].Physiol Rev,2008,88:287-290.
[9]Agius L.Role of glycogen phosphorylase in liver glycogen metabolism[J].Mol Aspects Med,2015,46:34-45.
[10]彭梅,张振东,杨娟.土党参多糖对小鼠的抗疲劳作用[J].食品科学,2011,32:224-226.
[11]Deng B,Sullivan MA,Chen C,et al.Molecular structure of human-liver glycogen[J].PLoS One,2016,11:e0150540.
[12]Jeukendrup AE.Carbohydrate and exercise performance:the role of multiple transportable carbohydrates[J].Curr Opin Clin Nutr,2010,13:452-457.
[13]张桂英,赵海军,信振江,等.枫糖对小鼠抗运动疲劳能力的影响[J].西安交通大学学报,2006,29:752-755.
[14]Cai AJ,Qi SW,Su ZW,et al.Method comparison and bias estimation of blood urea nitrogen(BUN),creatinine(Cr),and uric acid(UA)measurements between two analytical methods[J].Clin Lab,2017,63:73-77.
[15]Takaya Y,Yoshihara F,Yokoyama H,et al.Risk stratification of acute kidney injury using the blood urea nitrogen/creatinine ratio in patients with acute decompensated heart failure[J].Circ J,2015,79:1520-1525.
[16]董书清.血清肌酐、尿素氮的检测值及其比值与肾脏损害的探讨[J].中国医药指南,2011,9:136-137.
[17]刘亚林,王敬义,徐海,等.中药熏剂对运动机体血乳酸的影响[J].中国临床康复,2002,6:109-110.
[18]陈婷,李绪稳.运动对机体血清中乳酸脱氢酶活性的影响[J].体育世界(学术版),2015(4):142-143.
[19]Adeyemi OT,Osilesi O,Adebawo O,et al.Alkaline phosphatase(ALP),aspartate aminotransferase(AST)and alanine aminotransferase(ALT)activities in selected tissues of mice fed on processed atlantic horse mackerel(trachurus trachurus)[J].Adv Biosci Biotechnol,2015,6:139-152.
[20]Gurung RB,Purbe B,Gyawali P,et al.The Ratio of aspartate aminotransferase to alanine aminotransferase(AST/ALT):the corelation of value with underlying severity of alcoholic liver disease[J].Kathmandu Univ Med J,2013,43:233-236.
[2]麻懿馨,范文今,赵翠霞,等.抗疲劳中药对小鼠抗疲劳作用的实验研究[J].上海预防医学,2013,25:592-594.
[3]夏勇,赵硕,傅剑云.灵芝粉抗疲劳作用的实验研究[J].中国临床康复,2002,6:3618-3619.
[4]覃筱燕,唐丽,杨林,等.枸杞提取物对小鼠抗疲劳作用的实验研究[J].中医药学报,2009,37:8-11.
[5]关力.大脑疲劳的研究与进展[J].国外医学生物医学工程分册,1996,19:9-13.
[6]何来英,严卫星,楼密密,等.保健食品抗疲劳作用实验方法研究[J].中国食品卫生杂志,1997,9:1-7
[7]杨国宇,王艳玲,王月影,等.肌肽对小鼠抗疲劳作用的实验研究[J].河南农业大学学报,2003,37:383-385.
[8]Allen DG,Lamb GD,Westerblad H.Skeletal muscle fatigue:cellular mechanisms[J].Physiol Rev,2008,88:287-290.
[9]Agius L.Role of glycogen phosphorylase in liver glycogen metabolism[J].Mol Aspects Med,2015,46:34-45.
[10]彭梅,张振东,杨娟.土党参多糖对小鼠的抗疲劳作用[J].食品科学,2011,32:224-226.
[11]Deng B,Sullivan MA,Chen C,et al.Molecular structure of human-liver glycogen[J].PLoS One,2016,11:e0150540.
[12]Jeukendrup AE.Carbohydrate and exercise performance:the role of multiple transportable carbohydrates[J].Curr Opin Clin Nutr,2010,13:452-457.
[13]张桂英,赵海军,信振江,等.枫糖对小鼠抗运动疲劳能力的影响[J].西安交通大学学报,2006,29:752-755.
[14]Cai AJ,Qi SW,Su ZW,et al.Method comparison and bias estimation of blood urea nitrogen(BUN),creatinine(Cr),and uric acid(UA)measurements between two analytical methods[J].Clin Lab,2017,63:73-77.
[15]Takaya Y,Yoshihara F,Yokoyama H,et al.Risk stratification of acute kidney injury using the blood urea nitrogen/creatinine ratio in patients with acute decompensated heart failure[J].Circ J,2015,79:1520-1525.
[16]董书清.血清肌酐、尿素氮的检测值及其比值与肾脏损害的探讨[J].中国医药指南,2011,9:136-137.
[17]刘亚林,王敬义,徐海,等.中药熏剂对运动机体血乳酸的影响[J].中国临床康复,2002,6:109-110.
[18]陈婷,李绪稳.运动对机体血清中乳酸脱氢酶活性的影响[J].体育世界(学术版),2015(4):142-143.
[19]Adeyemi OT,Osilesi O,Adebawo O,et al.Alkaline phosphatase(ALP),aspartate aminotransferase(AST)and alanine aminotransferase(ALT)activities in selected tissues of mice fed on processed atlantic horse mackerel(trachurus trachurus)[J].Adv Biosci Biotechnol,2015,6:139-152.
[20]Gurung RB,Purbe B,Gyawali P,et al.The Ratio of aspartate aminotransferase to alanine aminotransferase(AST/ALT):the corelation of value with underlying severity of alcoholic liver disease[J].Kathmandu Univ Med J,2013,43:233-236.