电针对功能性消化不良大鼠胃窦AMPKα及mTOR的影响
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摘要
目的观察电针足三里对功能性消化不良(FD)大鼠腺苷酸激活蛋白激酶(AMPKα)、雷帕霉素靶蛋白(m TOR)表达的影响。方法将50只大鼠按随机数字表法分为正常组及造模组,其中正常组10只。将除正常组外的大鼠采用夹尾刺激法配合不规则饮食构建FD大鼠模型,将造模成功的大鼠再随机分为模型组和电针组,各10只。正常组、模型组大鼠与电针组一样束缚固定,但不进行电针干预。电针组给予电针足三里进行干预,每日1次,共10 d。观察各组大鼠日常表现,采用q RT-PCR检测各组大鼠胃窦组织中AMPKα和m TOR mRNA的表达;用Western blotting检测各组大鼠胃窦AMPKα、m TOR蛋白的表达。结果与正常组比较,模型组大鼠胃窦AMPKαmRNA以及蛋白的表达显著降低(P <0.05),mTOR m RNA以及蛋白的表达显著升高(P <0.05)。与模型组比较,电针组胃窦中AMPKαm RNA以及蛋白的表达明显升高(P <0.05),mTOR mRNA以及蛋白的表达明显降低(P <0.05)。结论电针足三里可改善FD大鼠的胃肠消化,增加FD大鼠胃窦AMPKαmRNA以及蛋白的表达水平,降低FD大鼠胃窦mTOR m RNA以及蛋白的表达水平。
Objective: To observe the effect of electroacupuncture at Zusanli on the expression of AMPKα and mTOR in rats with functional dyspepsia(FD). Methods: 50 rats were randomly divided into the normal group and the model group. Rats,except the normal group,were cultured with the tail-stimulation method and irregular diet to construct the FD rat model. The rats with successful modeling were randomly divided into the model group and EA group,10 rats in each. The normal group and the model group were restrained and fixed as EA group,but no EA intervention was performed. EA group was given EA at Zusanli for once/day for 10 days. The daily performance of each group was observed. The expression of AMPKα and m TOR mRNA in gastric antrum was detected by qRT-PCR. The expression of AMPKα and mTOR protein in gastric antrum of each group were detected by Western blot. Results: Compared with the normal group,the expression of AMPKα mRNA and protein in the gastric antrum of the model group was significantly decreased(P < 0.05),and the expression of m TOR m RNA and protein expression was significantly increased(P < 0.05). Compared with the model group,the expression of AMPKα m RNA and protein in the gastric antrum of the electroacupuncture group was significantly increased(P <0.05),and the expression of mTOR mRNA and protein was significantly decreased(P < 0.05). Conclusion: EA at Zusanli can improve the gastrointestinal digestion of FD rats,increase the expression of AMPKα mRNA and protein in gastric antrum of FD rats,and decrease the expression of m TOR m RNA and protein in gastric antrum of FD rats.
Objective: To observe the effect of electroacupuncture at Zusanli on the expression of AMPKα and mTOR in rats with functional dyspepsia(FD). Methods: 50 rats were randomly divided into the normal group and the model group. Rats,except the normal group,were cultured with the tail-stimulation method and irregular diet to construct the FD rat model. The rats with successful modeling were randomly divided into the model group and EA group,10 rats in each. The normal group and the model group were restrained and fixed as EA group,but no EA intervention was performed. EA group was given EA at Zusanli for once/day for 10 days. The daily performance of each group was observed. The expression of AMPKα and m TOR mRNA in gastric antrum was detected by qRT-PCR. The expression of AMPKα and mTOR protein in gastric antrum of each group were detected by Western blot. Results: Compared with the normal group,the expression of AMPKα mRNA and protein in the gastric antrum of the model group was significantly decreased(P < 0.05),and the expression of m TOR m RNA and protein expression was significantly increased(P < 0.05). Compared with the model group,the expression of AMPKα m RNA and protein in the gastric antrum of the electroacupuncture group was significantly increased(P <0.05),and the expression of mTOR mRNA and protein was significantly decreased(P < 0.05). Conclusion: EA at Zusanli can improve the gastrointestinal digestion of FD rats,increase the expression of AMPKα mRNA and protein in gastric antrum of FD rats,and decrease the expression of m TOR m RNA and protein in gastric antrum of FD rats.
引文
[1]中华医学会消化病学分会胃肠动力学组,中华医学会消化病学分会胃肠功能性疾病协作组.中国功能性消化不良专家共识意见(2015年,上海)[J].中华消化杂志,2016,36(4):217-229.
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[5]魏艳菊,张汉平.电针四肢腧穴为主治疗功能性消化不良临床对照研究[J].上海针灸杂志,2017,36(6):679-683.
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[9]王煜姣,凌江红,张钰琴,等.复合病因造模法制备功能性消化不良大鼠模型[J].世界华人消化杂志,2014,22(2):210-214.
[10]林文注,王佩.实验针灸学[M].上海:上海科学技术出版社,1999:88-89.
[11]王柳,申国明,王浩,等.电针胃俞募穴对功能性消化不良大鼠胃运动及迷走神经背核N-甲基-D-天冬氨酸和血清一氧化氮表达的影响[J].中国针灸,2018,38(3):285-290.
[12]杨云,徐派的,辛玉,等.神经降压素介导的脑-肠轴在电针治疗功能性消化不良大鼠中的作用[J].针刺研究,2016,41(1):35-39,50.
[13]宋晓红,刘明明,仉红刚.m TOR信号通路与相关疾病的研究进展[J].微循环学杂志,2018,28(3):64-70.
[14]曾毅,张红星.m TOR激动剂及其抑制剂在电针治疗功能性消化不良中的作用[J].辽宁中医杂志,2018,45(2):405-409.
[15]Hardie DG,Ross FA,Hawley SA.AMPK:a nutrient and energy sensor that maintains energy homeostasis[J].Nat Rev Mol Cell Biol,2012,13(4):251-262.
[16]Xiao B,Sanders MJ,Underwood E,et al.Structure of mammalian AMPK and its regulation by ADP[J].Nature,2011,472(7342):230-233.
[17]李知行,蓝丹纯,张弘弢,等.电针对胰岛素抵抗大鼠肝脏AMPK及ACC蛋白表达的影响[J].辽宁中医杂志,2017,44(4):864-867.
[18]龚美蓉,陈凤丽,曹晨,等.电针通过AMPK/PGC-1信号途径改善肥胖大鼠胰岛素抵抗和线粒体功能的研究[J].时珍国医国药,2017,28(5):1253-1255.
[19]Chiang PC,Lin SC,Pan S.Antroquinonol displays anticancer potential against human hepatocellular carcinoma cells:a crucial role of AMPK and m TOR pathways[J].Biochem Pharmacol,2010,79(2):162-171.
[20]聂容荣,潘泓华,文辉,等.电针刺激天枢穴对急性重症胰腺炎大鼠肺组织AQP-1表达的影响[J].山东医药,2017,57(22):35-37.
[2]Tack J,Talley NJ,Camilleri M,et al.Functional gastroduodenal disorders[J].Gastroenterology,2016,130(5):1466-1479.
[3]张国山,刘密,邱冉冉,等.艾灸与针刺调节功能性消化不良大鼠胃肠动力障碍的对比研究[J].时珍国医国药,2016,27(11):2796-2798.
[4]严开伟,赵凌,杨洁,等.针刺治疗功能性消化不良机制研究进展[J].中国针灸,2015,35(9):973-976.
[5]魏艳菊,张汉平.电针四肢腧穴为主治疗功能性消化不良临床对照研究[J].上海针灸杂志,2017,36(6):679-683.
[6]康朝霞,张红星,徐派的,等.电针对功能性消化不良大鼠胃窦ghrelin及mTOR的影响[J].世界华人消化杂志,2017,25(17):1553-1557.
[7]Xu G,Li Y,An W,et al.Gastric mammalian target of rapamycin signaling regulates ghrelin production and food intake[J].Endocrinology,2009,150(8):3673-3644.
[8]林静,夏有兵,卢明香,等.电针对食源性肥胖大鼠p AMP-Kα表达的影响[J].新中医,2012,44(2):105-107.
[9]王煜姣,凌江红,张钰琴,等.复合病因造模法制备功能性消化不良大鼠模型[J].世界华人消化杂志,2014,22(2):210-214.
[10]林文注,王佩.实验针灸学[M].上海:上海科学技术出版社,1999:88-89.
[11]王柳,申国明,王浩,等.电针胃俞募穴对功能性消化不良大鼠胃运动及迷走神经背核N-甲基-D-天冬氨酸和血清一氧化氮表达的影响[J].中国针灸,2018,38(3):285-290.
[12]杨云,徐派的,辛玉,等.神经降压素介导的脑-肠轴在电针治疗功能性消化不良大鼠中的作用[J].针刺研究,2016,41(1):35-39,50.
[13]宋晓红,刘明明,仉红刚.m TOR信号通路与相关疾病的研究进展[J].微循环学杂志,2018,28(3):64-70.
[14]曾毅,张红星.m TOR激动剂及其抑制剂在电针治疗功能性消化不良中的作用[J].辽宁中医杂志,2018,45(2):405-409.
[15]Hardie DG,Ross FA,Hawley SA.AMPK:a nutrient and energy sensor that maintains energy homeostasis[J].Nat Rev Mol Cell Biol,2012,13(4):251-262.
[16]Xiao B,Sanders MJ,Underwood E,et al.Structure of mammalian AMPK and its regulation by ADP[J].Nature,2011,472(7342):230-233.
[17]李知行,蓝丹纯,张弘弢,等.电针对胰岛素抵抗大鼠肝脏AMPK及ACC蛋白表达的影响[J].辽宁中医杂志,2017,44(4):864-867.
[18]龚美蓉,陈凤丽,曹晨,等.电针通过AMPK/PGC-1信号途径改善肥胖大鼠胰岛素抵抗和线粒体功能的研究[J].时珍国医国药,2017,28(5):1253-1255.
[19]Chiang PC,Lin SC,Pan S.Antroquinonol displays anticancer potential against human hepatocellular carcinoma cells:a crucial role of AMPK and m TOR pathways[J].Biochem Pharmacol,2010,79(2):162-171.
[20]聂容荣,潘泓华,文辉,等.电针刺激天枢穴对急性重症胰腺炎大鼠肺组织AQP-1表达的影响[J].山东医药,2017,57(22):35-37.