UPLC-UV法同时测定葛酮通络胶囊中的6种活性成分
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摘要
【目的】应用超高液相色谱—紫外(UPLC-UV)法同时测定葛酮通络胶囊中6种活性成分。【方法】采用WatersACQUITY UPLC系统,BEH Shielded RP-C18色谱柱(2.1 mm×100 mm,1.7μm),柱温35℃,流动相采用乙腈和0.1%甲酸水,流速为0.2 mL·min-1。紫外检测波长为250 nm。【结果】所选6种活性成分可以同时实现分离并定量,所有6种成分的标准曲线的相关系数都大于0.998。6种成分的平均加样回收率及相对标准偏差(s R)分别为:3’-羟基葛根素,100.29,1.21%;葛根素,100.11,1.40%;大豆苷,99.73,1.15%;染料木苷,99.93,1.24%;大豆苷元,100.99,1.31%;染料木素,99.68,1.04%。【结论】本方法准确、快速、重复性好,可以作为全面控制葛酮通络胶囊质量的新方法。
Objective To establish a method for the simultaneous determination of 6 kinds of effective compoundsin Getong Tongluo Capsules by ultra-performance liquid chromatography-ultraviolet(UPLC-UV). Methods The chromatography was performed with Waters ACQUITY UPLC system on a BEH shielded RP-C18 column(2.1 mm × 100 mm,1.7 μm) at temperature of 35 ℃. The mobile phase consisted of acetonitrile and 0.1% formicacid with the flow rate of 0.2 mL·min-1. The UV detection wavelength was 250 nm. Results All of 6 kinds of activecompounds were simultaneously separated and quantified,and their correlation coefficients in calibration curveswere more than 0.998.Their average recovery and relative standard deviation(RSD,s R) of the 6 kinds of activecompounds were listed as follows:3'-hydroxy puerarin,100.29,1.21%;puerarin,100.11,1.40%;daidzin,99.73,1.15%;genistin,99.93,1.24%;daidzein,100.99,1.31%;genistein,99.68,1.04%. Conclusion The validated UPLC-UV is a new method for the quality control of Getong Tongluo capsules,which is accurate,rapid,and reproducible.
Objective To establish a method for the simultaneous determination of 6 kinds of effective compoundsin Getong Tongluo Capsules by ultra-performance liquid chromatography-ultraviolet(UPLC-UV). Methods The chromatography was performed with Waters ACQUITY UPLC system on a BEH shielded RP-C18 column(2.1 mm × 100 mm,1.7 μm) at temperature of 35 ℃. The mobile phase consisted of acetonitrile and 0.1% formicacid with the flow rate of 0.2 mL·min-1. The UV detection wavelength was 250 nm. Results All of 6 kinds of activecompounds were simultaneously separated and quantified,and their correlation coefficients in calibration curveswere more than 0.998.Their average recovery and relative standard deviation(RSD,s R) of the 6 kinds of activecompounds were listed as follows:3'-hydroxy puerarin,100.29,1.21%;puerarin,100.11,1.40%;daidzin,99.73,1.15%;genistin,99.93,1.24%;daidzein,100.99,1.31%;genistein,99.68,1.04%. Conclusion The validated UPLC-UV is a new method for the quality control of Getong Tongluo capsules,which is accurate,rapid,and reproducible.
引文
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[3]邬淼林,陈红林,蔡荣华.葛酮通络胶囊治疗椎动脉型颈椎病的效果研究[J].按摩与康复医学,2017,8(18):62.
[4]李静,夏成凯,方成武. HPLC法测定葛酮通络胶囊中葛根素含量[J].广州化工,2016,44(4):78.
[5]牛海军,李晓亮,李光辉,等.葛酮通络胶囊HPLC指纹图谱研究[J].安徽医药,2016,20(2):249.
[6]郭利民,钟世华,苏巧玲.离子络合紫外分光光度法测定葛酮通络胶囊中葛根素的含量[J].中国现代药物应用,2009,3(1):36.
[7] Guerra M C,Speroni E,Broccoli M,et al. Comparison between Chinese medical herb Pueraria lobata crude extract and its main isoflavone puerarin:Antioxidant properties and effects on rat liver CYP-catalysed drug metabolism[J]. Life Sciences, 2000, 67(24):2997.
[8]蔡少青,王璇,尚明英,等.中药“显效理论”或有助于阐释并弘扬中药特色优势[J].中国中药杂志,2015,40(17):3435.