蛋白质含量对天然胶乳中橡胶粒子稳定性的影响
|
摘要
天然胶乳(NRL)中主要成分为橡胶烃、水和蛋白质、类脂物、无机盐等非胶组分,其中蛋白质对天然胶乳橡胶粒子形态和稳定性有重要影响。对天然胶乳进行离心及酶解处理,得到蛋白质含量不同的脱蛋白胶乳(DPNR)。研究蛋白质对胶乳粒径、zeta电位、胶膜凝胶含量和交联密度的影响。通过对比分析,离心及酶处理后,蛋白质含量降低,平均粒径增大,zeta电位降低,凝胶含量和交联密度均降低。结果表明随着蛋白质含量的降低,橡胶粒子发生团聚,粒子表面保护层完整性和分子网络结构被破坏,分子链间交联点减少,稳定性降低。
The main components of natural rubber latex(NRL) are rubber hydrocarbon, water and protein, lipids, inorganic salts and other non-rubber components, and protein has an important influence on the morphology and stability of natural rubber latex particles. In this paper, the deproteinized natural rubber latex(DPNR) with different protein contents treated by centrifugal and enzyme treatment were obtained. The effect of protein on rubber particle size, Zeta potential, gel content and crosslink density were investigated. Through comparative analysis, after centrifugation and enzyme treatment, the protein content decreased, the average particle size increased, the zeta potential decreased, and the gel content and crosslink density decreased. The results show that as the protein content decreases, the rubber particles agglomerate, the surface protective layer integrity and molecular network structure of the particles are destroyed, the cross-linking points between the molecular chains are reduced and the stability is lowered.
The main components of natural rubber latex(NRL) are rubber hydrocarbon, water and protein, lipids, inorganic salts and other non-rubber components, and protein has an important influence on the morphology and stability of natural rubber latex particles. In this paper, the deproteinized natural rubber latex(DPNR) with different protein contents treated by centrifugal and enzyme treatment were obtained. The effect of protein on rubber particle size, Zeta potential, gel content and crosslink density were investigated. Through comparative analysis, after centrifugation and enzyme treatment, the protein content decreased, the average particle size increased, the zeta potential decreased, and the gel content and crosslink density decreased. The results show that as the protein content decreases, the rubber particles agglomerate, the surface protective layer integrity and molecular network structure of the particles are destroyed, the cross-linking points between the molecular chains are reduced and the stability is lowered.
引文
[1] Wititsuwannakul R,Rukseree K,Kanokwiroon K.A rubber particle protein specific for Hevea latex lectin binding involved in latex coagulation[J].Phytochemistry,2008,69(5):1111-1118.
[2] 何映平.天然橡胶加工学[M].海南:海南出版社,2007.
[3] Woods J A,Lambert S,Platts-Mills T A E,et al.Natural rubber latex allergy:spectrum,diagnostic approach,and therapy[J].Journal of Emergency Medicine.1997,15(1):71-85.
[4] Sakdapipanich J.Current study on structural characterization and unique film formation of hevea brasiliensis natural rubber latex[J].Advanced Materials Research,2014,844:498-501.
[5] Sussman G L,Beezhold D H,Kurup V P.Allergens and natural rubber proteins[J].J Allergy Clin Immunol,2002,110(2):S33-S39.
[6] Michelle S B,Klaus E A,Carsten B J,et al.Decrease in the rate of sensitization and clinical allergy to natural rubber latex[J].Contact Dermatitis,2015,73(1):21-28.
[7] Steven L K,Joshua O P,Vassilios A D,et al.Natural rubber latex allergy [J].DM Diseasea- Month,2016,62(1):5-17.
[8] Franziska R.Sensitization and allergy to natural rubber latex[J].Encyclopedia of Medical Immunology,2015,105(1):615-619.
[9] Amnuaypornsri S,Sakdapipanich J,Toki S.Strain-induced crystallization of natural rubber:effect of proteins and phospholipids[J].Rubber Chemistry & Technology,2008,81(5):753-766.
[10] Cai Keping,Huang Shengliang,Li Puwang,et al.Performance and application of domestic deproteinized natural rubber[J].Rubber Technology,2013,11(11):25-28( in Chinese).蔡克平,黄胜良,李普旺,等.国产脱蛋白天然橡胶的性能及应用[J].橡胶科技,2013,11(11):25-28.
[11] Chen Yuanyuan,Zhao Tongjian,Fu Xin,et al.Study on the effect of protein on natural rubber and its properties[J].Elastomer,2008,18(6):58-60(in Chinese).陈媛媛,赵同建,符新,等.蛋白质对天然橡胶及其性能影响的研究[J].弹性体,2008,18(6):58-60.
[12] Liu Huali.New breakthrough in anti-protein allergy technology[J].Latex Industry,1995,(3):39-42( in Chinese).刘华力.抗蛋白质过敏技术的新突破[J].胶乳工业,1995,(3):39-42 .
[13] Yamamoto Y,Nghia,W Klinklai P T,et al.Removal of proteins from natural rubber with urea amd its application to continuous processes[J].Journal of Applied Polymer Science,2008,107(4):2329-2332.
[14] Lucksanaporn Tarachiwin,Jitladda Sakdapipanich.Structural characterization of alpha-terminal group of natural rubber 2 .Decomposition of branch-points by phospholipase and chemical treatments[J].Biomacromolecules,2005,6(4):1858-1863.
[15] 吴龙梅.天然胶乳橡胶粒子结构及分子链网络结构的研究[D].海口:海南大学,2015.
[16] Bai Xiaoying,Zhang Huifeng,Jin Linhe,et al.Effect of electrolyte and acidity and alkali on the electric properties of natural fresh latex[J].Materials review,2016,30(3):61-65(in Chinese).白晓莹,张会丰,金林赫,等.电解质和酸碱性对天然鲜胶乳电动特性的影响[J].材料导报,2016,30(3):61-65.
[17] Lei Genzhu,Ma Mingyue,Zhang Zhie,et al.Analysis of the difference in quality of natural concentrated latex at home and abroad[J].Polymer Bulletin,2018,4,79-87(in Chinese).雷根珠,马明月,张志娥,等.国内外天然浓缩胶乳品质差异性分析[J].高分子通报,2018,4:79-87.
[18] Amnuaypornsri S,Tarachiwin L,Sakdapipanich J T.Character of long-chain branching in highly purified natural rubber[J].Journal of Applied Polymer Science,2010,115 (6):3645-3650.
[19] Puskasa J E,Gautriauda E,Deffieuxb A.Natural rubber biosynthesis-A living carbocationic polymerization[J].Progress in Polymer Science,2006,31 (6):533-548.
[20] Tuampoemsab S,Sakdapipanich J,Tanaka Y.Influence of some Non-Rubber Components on Aging Behavior of Purified Natural Rubber[J].Rubber Chemistry & Technology,2007,80 (1):159-168.
[21] Nawamat K,Sakdapipanich J T,Ho C C.Effect of deproteinized methods on the proteins and properties of natural rubber latex during storage[J].Macromelcular Symposia,2010,288(1):95-103.
[22] Suchiva K,Kowitteerawut T,Srichantamit L.Structure properties of purified natural rubber[J].Journal of Applied Polymer Science,2015,78(8):1495-1504.
[2] 何映平.天然橡胶加工学[M].海南:海南出版社,2007.
[3] Woods J A,Lambert S,Platts-Mills T A E,et al.Natural rubber latex allergy:spectrum,diagnostic approach,and therapy[J].Journal of Emergency Medicine.1997,15(1):71-85.
[4] Sakdapipanich J.Current study on structural characterization and unique film formation of hevea brasiliensis natural rubber latex[J].Advanced Materials Research,2014,844:498-501.
[5] Sussman G L,Beezhold D H,Kurup V P.Allergens and natural rubber proteins[J].J Allergy Clin Immunol,2002,110(2):S33-S39.
[6] Michelle S B,Klaus E A,Carsten B J,et al.Decrease in the rate of sensitization and clinical allergy to natural rubber latex[J].Contact Dermatitis,2015,73(1):21-28.
[7] Steven L K,Joshua O P,Vassilios A D,et al.Natural rubber latex allergy [J].DM Diseasea- Month,2016,62(1):5-17.
[8] Franziska R.Sensitization and allergy to natural rubber latex[J].Encyclopedia of Medical Immunology,2015,105(1):615-619.
[9] Amnuaypornsri S,Sakdapipanich J,Toki S.Strain-induced crystallization of natural rubber:effect of proteins and phospholipids[J].Rubber Chemistry & Technology,2008,81(5):753-766.
[10] Cai Keping,Huang Shengliang,Li Puwang,et al.Performance and application of domestic deproteinized natural rubber[J].Rubber Technology,2013,11(11):25-28( in Chinese).蔡克平,黄胜良,李普旺,等.国产脱蛋白天然橡胶的性能及应用[J].橡胶科技,2013,11(11):25-28.
[11] Chen Yuanyuan,Zhao Tongjian,Fu Xin,et al.Study on the effect of protein on natural rubber and its properties[J].Elastomer,2008,18(6):58-60(in Chinese).陈媛媛,赵同建,符新,等.蛋白质对天然橡胶及其性能影响的研究[J].弹性体,2008,18(6):58-60.
[12] Liu Huali.New breakthrough in anti-protein allergy technology[J].Latex Industry,1995,(3):39-42( in Chinese).刘华力.抗蛋白质过敏技术的新突破[J].胶乳工业,1995,(3):39-42 .
[13] Yamamoto Y,Nghia,W Klinklai P T,et al.Removal of proteins from natural rubber with urea amd its application to continuous processes[J].Journal of Applied Polymer Science,2008,107(4):2329-2332.
[14] Lucksanaporn Tarachiwin,Jitladda Sakdapipanich.Structural characterization of alpha-terminal group of natural rubber 2 .Decomposition of branch-points by phospholipase and chemical treatments[J].Biomacromolecules,2005,6(4):1858-1863.
[15] 吴龙梅.天然胶乳橡胶粒子结构及分子链网络结构的研究[D].海口:海南大学,2015.
[16] Bai Xiaoying,Zhang Huifeng,Jin Linhe,et al.Effect of electrolyte and acidity and alkali on the electric properties of natural fresh latex[J].Materials review,2016,30(3):61-65(in Chinese).白晓莹,张会丰,金林赫,等.电解质和酸碱性对天然鲜胶乳电动特性的影响[J].材料导报,2016,30(3):61-65.
[17] Lei Genzhu,Ma Mingyue,Zhang Zhie,et al.Analysis of the difference in quality of natural concentrated latex at home and abroad[J].Polymer Bulletin,2018,4,79-87(in Chinese).雷根珠,马明月,张志娥,等.国内外天然浓缩胶乳品质差异性分析[J].高分子通报,2018,4:79-87.
[18] Amnuaypornsri S,Tarachiwin L,Sakdapipanich J T.Character of long-chain branching in highly purified natural rubber[J].Journal of Applied Polymer Science,2010,115 (6):3645-3650.
[19] Puskasa J E,Gautriauda E,Deffieuxb A.Natural rubber biosynthesis-A living carbocationic polymerization[J].Progress in Polymer Science,2006,31 (6):533-548.
[20] Tuampoemsab S,Sakdapipanich J,Tanaka Y.Influence of some Non-Rubber Components on Aging Behavior of Purified Natural Rubber[J].Rubber Chemistry & Technology,2007,80 (1):159-168.
[21] Nawamat K,Sakdapipanich J T,Ho C C.Effect of deproteinized methods on the proteins and properties of natural rubber latex during storage[J].Macromelcular Symposia,2010,288(1):95-103.
[22] Suchiva K,Kowitteerawut T,Srichantamit L.Structure properties of purified natural rubber[J].Journal of Applied Polymer Science,2015,78(8):1495-1504.