肉桂油对致痤疮菌抑制作用的研究
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摘要
目的研究肉桂油对致痤疮菌的抑制作用。方法采用纸片扩散法测定抑菌环大小和通过微量肉汤稀释法分别测定肉桂油对于4种致痤疮菌的最小抑菌浓度(MIC)。结果 4种致痤疮菌对肉桂油的敏感程度均属于极敏;肉桂油对痤疮丙酸杆菌的MIC为0.156μL/mL,对表皮葡萄球菌和金黄色葡萄球菌的MIC为0.625μL/mL,对糠秕马拉色菌的MIC为0.313μL/mL。结论结果表明了肉桂油对致痤疮菌具有较好的抑制效果,本实验的测定结果可为肉桂油作为一种高效、不易使微生物产生耐药性、低成本、绿色的药物应用于痤疮治疗提供实验依据。
Objective To study the bacteriostatic effect of cinnamon oil on acne-inducing bacteria.Methods The size of inhibitory ring was determined by K-B disc diffusion method and the minimum inhibitory concentration(MIC) of cinnamon oil against four acne-causing bacteria was determined by broth dilution method.Results The sensitivity of four acne-causing bacteria(Propionibacterium acnes,Staphylococcus epidermidis,Staphylococcus aureus and Malassezia furfur) to cinnamon oil was extremely sensitive.The MIC of cinnamon oil on Propionibacterium acnes,Malassezia furfur,Staphylococcus epidermidis and Staphylococcus aureus were 0.156 μL/mL,0.313 μL/mL,0.625 μL/mL and 0.625 μL/mL,respectively.Conclusion Cinnamon oil exhibits a good inhibitory effect on acne bacteria,which may provide a reference for use of cinnamon oil as a highly effective,low-cost and green drug for acne treatment.
Objective To study the bacteriostatic effect of cinnamon oil on acne-inducing bacteria.Methods The size of inhibitory ring was determined by K-B disc diffusion method and the minimum inhibitory concentration(MIC) of cinnamon oil against four acne-causing bacteria was determined by broth dilution method.Results The sensitivity of four acne-causing bacteria(Propionibacterium acnes,Staphylococcus epidermidis,Staphylococcus aureus and Malassezia furfur) to cinnamon oil was extremely sensitive.The MIC of cinnamon oil on Propionibacterium acnes,Malassezia furfur,Staphylococcus epidermidis and Staphylococcus aureus were 0.156 μL/mL,0.313 μL/mL,0.625 μL/mL and 0.625 μL/mL,respectively.Conclusion Cinnamon oil exhibits a good inhibitory effect on acne bacteria,which may provide a reference for use of cinnamon oil as a highly effective,low-cost and green drug for acne treatment.
引文
[1]吴贇,吉杰,张玲琳,等.微生物在痤疮发病中的作用[J].中国皮肤性病学杂志,2016,30(3):311-314.
[2]陈述文,何国强,蔡常辉.痤疮皮损涂片镜检细菌的临床应用[J].实验与检验医学,2015,33(3):331-333.
[3]RIGOPOULOS D,LARIOS G,KATSAMBAS A D,et al.The role of isotretinoin in acne therapy:why not as first--line therapy facts and controversies[J].Clin Dermatol,2010,28(1):24-30.
[4]尹兴平,夏隆庆.痤疮患者痤疮丙酸杆菌耐药性的研究进展[J].国外医学.皮肤性病学分册,2004,30(3):152-154.
[5]谢朝云,熊芸,孙静,等.表皮葡萄球菌与金黄色葡萄球菌感染分布及耐药性分析[J].中华医院感染学杂志,2015,25(22):5083-5085.
[6]黄晓晖,陈思东,李庭杰.有机物对肉桂油杀菌效果的影响[J].中国预防医学杂志,2013,14(10):748-750.
[7]黄晓晖,陈思东,李庭杰,等.肉桂油水溶液及其乳液杀菌作用研究[J].中国消毒学杂志,2014,31(6):582-584.
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[12]陈立平,张慧萍,陈光,等.肉桂油成分分析及肉桂醛体外抗肿瘤活性研究[J].中国微生态学杂志,2012,24(4):327-330.
[13]李唯佳,王绪平,俞忠明,等.肉桂挥发油对糖尿病大鼠血糖、血脂的影响[J].中国中医药科技,2012,19(1):37-38.
[14]牟海栋,屠鹏飞,姜勇,等.基于网络药理学的肉桂温经通脉的作用机制研究[J].药学学报,2016,51(11):1724-1733.
[15]黄敬群,罗晓星,王四旺,等.桂皮醛对抗血小板聚集和血栓形成的特点[J].中国临床康复,2006,10(31):34-36.
[16]周本杰,李亚冰.退热止痛散解热镇痛活性部位气相色谱-质谱分析[J].时珍国医国药,2011,22(10):2457-2458.
[17]吴凡,贾汝汉.肉桂提取物的药理作用研究进展[J].医药导报,2012,31(7):882-885
[18]任顺成.黄连、肉桂不同溶剂萃取物的抑菌性和抗氧化性[C]//Intelligent Information Technology Application Association.Agricultural and natural resources engineering(ANRE 2011ABE V3).Intelligent Information Technology Application Association,2011.
[19]顾仁勇,傅伟昌,李佑稷,等.肉桂精油抑菌及抗氧化作用的研究[J].食品研究与开发,2008,29(10):29-32.
[20]MOUNIA O,STPHANE C,LINDA S,et al.Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria:E.coli O157:H7,Salmonella typhimurium,Staphylococcus aureus and Listeria monocytogenes[J].Food Control,2015,18(5):414-420.
[21]李品艾,丁素君.肉桂挥发油的抗氧化作用研究[J].安徽农业科学,2010,38(24):13547-13548,13551.
[22]DING yuanyuan,ZHAO gangtao,YANG Fan,et al.Influence of oleum cinnamomion coxsackievirus B-3-Induced viral myocarditisin mice by inhibiting TLR4-NF-k-b signal pathway[J].Chin Pharmac,2010,45(5):348.
[23]刘晓艳,白卫东.肉桂油抑菌及抗氧化作用的研究进展[J].食品与机械,2010,26(5):169-172.
[24]唐裕芳,张妙玲,黄白飞,等.肉桂油的提取及其抑菌活性研究[J].天然产物研究与开发,2006,18(3):73-75.
[2]陈述文,何国强,蔡常辉.痤疮皮损涂片镜检细菌的临床应用[J].实验与检验医学,2015,33(3):331-333.
[3]RIGOPOULOS D,LARIOS G,KATSAMBAS A D,et al.The role of isotretinoin in acne therapy:why not as first--line therapy facts and controversies[J].Clin Dermatol,2010,28(1):24-30.
[4]尹兴平,夏隆庆.痤疮患者痤疮丙酸杆菌耐药性的研究进展[J].国外医学.皮肤性病学分册,2004,30(3):152-154.
[5]谢朝云,熊芸,孙静,等.表皮葡萄球菌与金黄色葡萄球菌感染分布及耐药性分析[J].中华医院感染学杂志,2015,25(22):5083-5085.
[6]黄晓晖,陈思东,李庭杰.有机物对肉桂油杀菌效果的影响[J].中国预防医学杂志,2013,14(10):748-750.
[7]黄晓晖,陈思东,李庭杰,等.肉桂油水溶液及其乳液杀菌作用研究[J].中国消毒学杂志,2014,31(6):582-584.
[8]中华人民共和国卫生部.消毒技术规范[M].北京:中华人民共和国卫生部,2002:83-84.
[9]Clinical And Laboratory Standards Institute.Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically:Approved standard[M].Ninth Edition.Wayne:2012:07-A9.
[10]COCKERILL F R.Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically:Approved standard[M].Ninth Edition.Wayne:CLSI,2012.
[11]《中国森林》编辑委员会.中国森林[M].北京:中国林业出版社,2000:113-118.
[12]陈立平,张慧萍,陈光,等.肉桂油成分分析及肉桂醛体外抗肿瘤活性研究[J].中国微生态学杂志,2012,24(4):327-330.
[13]李唯佳,王绪平,俞忠明,等.肉桂挥发油对糖尿病大鼠血糖、血脂的影响[J].中国中医药科技,2012,19(1):37-38.
[14]牟海栋,屠鹏飞,姜勇,等.基于网络药理学的肉桂温经通脉的作用机制研究[J].药学学报,2016,51(11):1724-1733.
[15]黄敬群,罗晓星,王四旺,等.桂皮醛对抗血小板聚集和血栓形成的特点[J].中国临床康复,2006,10(31):34-36.
[16]周本杰,李亚冰.退热止痛散解热镇痛活性部位气相色谱-质谱分析[J].时珍国医国药,2011,22(10):2457-2458.
[17]吴凡,贾汝汉.肉桂提取物的药理作用研究进展[J].医药导报,2012,31(7):882-885
[18]任顺成.黄连、肉桂不同溶剂萃取物的抑菌性和抗氧化性[C]//Intelligent Information Technology Application Association.Agricultural and natural resources engineering(ANRE 2011ABE V3).Intelligent Information Technology Application Association,2011.
[19]顾仁勇,傅伟昌,李佑稷,等.肉桂精油抑菌及抗氧化作用的研究[J].食品研究与开发,2008,29(10):29-32.
[20]MOUNIA O,STPHANE C,LINDA S,et al.Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria:E.coli O157:H7,Salmonella typhimurium,Staphylococcus aureus and Listeria monocytogenes[J].Food Control,2015,18(5):414-420.
[21]李品艾,丁素君.肉桂挥发油的抗氧化作用研究[J].安徽农业科学,2010,38(24):13547-13548,13551.
[22]DING yuanyuan,ZHAO gangtao,YANG Fan,et al.Influence of oleum cinnamomion coxsackievirus B-3-Induced viral myocarditisin mice by inhibiting TLR4-NF-k-b signal pathway[J].Chin Pharmac,2010,45(5):348.
[23]刘晓艳,白卫东.肉桂油抑菌及抗氧化作用的研究进展[J].食品与机械,2010,26(5):169-172.
[24]唐裕芳,张妙玲,黄白飞,等.肉桂油的提取及其抑菌活性研究[J].天然产物研究与开发,2006,18(3):73-75.