胃癌患者Sox2蛋白和CTHRC-1蛋白的表达与肿瘤发展密切相关
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摘要
本研究选取病理科收集的自2016年1月至2016年12月的90例术后胃癌组织标本(胃癌组)、45例癌旁组织标本(对照组),采用免疫组化染色法检测两组标本中的Y框蛋白(Sox2)、胶原三股螺旋重叠蛋白-1表达,并分析其与肿瘤TNM分期、淋巴结转移、浸润深度、分化程度的关系,采用Spearman秩相关检验检测两种蛋白的相互关系,试图探讨胃癌患者癌组织中性别决定区Y框蛋白(Sox2)、胶原三股螺旋重叠蛋白-1(CTHRC-1)的表达及其相关性。研究结果表明:胃癌组的Sox2蛋白阳性表达率63.33%显著低于对照组的93.33%(p<0.05),胃癌组的CTHRC-1蛋白阳性表达率78.89%显著高于对照组的24.44%(p<0.05);胃癌组的Sox2蛋白阳性表达与胃癌的分化程度、发生淋巴结转移具有相关性(p<0.05);胃癌组的CTHRC-1蛋白阳性表达与胃癌的淋巴结转移、TNM分期、肿瘤浸润深度具有显著的相关性(p<0.05);胃癌组的Sox2蛋白与CTHRC-1蛋白呈显著的负相关表达(Spearman相关系数r=-0.322, p=0.002<0.05)。本研究的初步结论表明:胃癌组织中Sox2蛋白低表达和CTHRC-1蛋白高表达,并且与肿瘤发展密切相关,且二者表达呈负相关。
In this research, totally 90 specimens of gastric cancer tissues(the gastric cancer group) and 45 specimens of paracancerous tissues(the control group) collected from pathology department were selected, which were from January 2016 to December 2016. The expression of Y-box protein(Sox2) and CTHRC-1 protein in the specimens were analyzed by the way of immunohistochemical staining. The relationship between Y-box protein(Sox2),CTHRC-1 protein, TNM staging, lymph node metastasis, depth of invasion and differentiation degree was analyzed.Spearman rank correlation test was used to detect the correlation between the two proteins in order to investigate the expression and correlation of sex determining Y box protein and CTHRC-1 protein. It was found that the positive rate of Sox2 protein in the gastric cancer group was 63.33%, which was significantly lower than that in the control group(93.33%, p<0.05). The positive rate of CTHRC-1 protein in the gastric cancer group was 78.89%, which was significantly higher than that in the control group(24.44%, p<0.05). The positive expression of Sox2 protein in the gastric cancer group was correlated with the differentiation degree and lymph node metastasis(p<0.05). The positive expression of CTHRC-1 protein in gastric cancer group was significantly correlated with the lymph node metastasis,TNM stage, tumor infiltration(p<0.05). There was a significant negative correlation between Sox2 protein and CTHRC-1 protein in the gastric cancer group(Spearman correlation coefficient r=-0.322, p=0.002<0.05). It could be concluded that the low expression of Sox2 protein and the high expression of CTHRC-1 protein in gastric cancer tissues were highly correlated with tumor progression. The expression of STH2 protein was negatively correlated.
In this research, totally 90 specimens of gastric cancer tissues(the gastric cancer group) and 45 specimens of paracancerous tissues(the control group) collected from pathology department were selected, which were from January 2016 to December 2016. The expression of Y-box protein(Sox2) and CTHRC-1 protein in the specimens were analyzed by the way of immunohistochemical staining. The relationship between Y-box protein(Sox2),CTHRC-1 protein, TNM staging, lymph node metastasis, depth of invasion and differentiation degree was analyzed.Spearman rank correlation test was used to detect the correlation between the two proteins in order to investigate the expression and correlation of sex determining Y box protein and CTHRC-1 protein. It was found that the positive rate of Sox2 protein in the gastric cancer group was 63.33%, which was significantly lower than that in the control group(93.33%, p<0.05). The positive rate of CTHRC-1 protein in the gastric cancer group was 78.89%, which was significantly higher than that in the control group(24.44%, p<0.05). The positive expression of Sox2 protein in the gastric cancer group was correlated with the differentiation degree and lymph node metastasis(p<0.05). The positive expression of CTHRC-1 protein in gastric cancer group was significantly correlated with the lymph node metastasis,TNM stage, tumor infiltration(p<0.05). There was a significant negative correlation between Sox2 protein and CTHRC-1 protein in the gastric cancer group(Spearman correlation coefficient r=-0.322, p=0.002<0.05). It could be concluded that the low expression of Sox2 protein and the high expression of CTHRC-1 protein in gastric cancer tissues were highly correlated with tumor progression. The expression of STH2 protein was negatively correlated.
引文
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Guo K.,Zhou M.G.,and Pan X.P.,2015,Trends and characteristics of gastric cancer mortality in China,2006~2012,Zhongguo Gonggong Weisheng(Chinese Journal of Public Health),31(3):341-343(郭康,周脉耕,潘晓平,2015,中国居民2006-2012年胃癌死亡趋势分析,中国公共卫生,31(3):341-343)
Kodera Y.,Isobe K.,Yamauchi M.,Kondoh K.,Kimura N.,Akiyama S.,Itoh K.,Nakashima I.,and Takagi H.,2015,Expression of nm23 H-1 RNA levels in human gastric cancer tissues,a negative correlation with nodal metastasis,Cancer,73(2):259-265
Lang L.,Zhou M.L.,Xu L.Y.,Du Y.E.,Wen S.Y.,and Liu M.R.,2016,TGF-βsignaling pathway regulates the mammosphere formation and migration of highly Twist-expressed breast cancer cells,Zhongliu(Tumor),36(10):1083-1089(郎磊,周明莉,徐丽云,杜燕娥,文思阳,柳满然,2016,TGF-β信号通路调控Twist高表达型乳腺肿瘤细胞微球体的形成及迁移,肿瘤,36(10):1083-1089)
Li Y.X.,Li X.M.,Zhang N.,Zhang W.,Chen C.,Tao L.,Zhao J.,Li S.G.,and Li F.,2015,Associations of Helicobacter pylori infection with the pathogenesis,progression and prognosis in patients with gastric cancer,Zhonghua Zhongliu Yufang Zazhi(Chinese Journal of Cancer Prevention and Treatment),22(2):91-94(李一鑫,李秀明,张楠,张微,陈成,陶林,赵瑾,李述刚,李锋,2015,幽门螺杆菌感染与胃癌发生发展及预后的相关性研究,中华肿瘤防治杂志,22(2):91-94)
Shuai X.M.,Gao J.B.,Liu J.H.,Zhang P.,Bai J.,Cai K.L.,Wang G.B.,and Tao K.X.,2015,Clinical efficacy of neoadjuvant chemotherapy combined with laparoscopy-assisted radical gastrectomy for advanced gastric cancer,Zhonghua Xiaohua Waike Zazhi(Chinese Journal of Digestive Surgery),15(3):241-246(帅晓明,高金波,刘兴华,张鹏,白洁,蔡开琳,王国斌,陶凯雄,2016,新辅助化疗联合腹腔镜辅助胃癌根治术治疗进展期胃癌的疗效分析,中华消化外科杂志,15(3):241-246)
Song H.,Liu X.H.,Shen Z.H.,Chen Q.,Xu F.X.,and Xu L.M.,2015,Research on the clinical significance of CTHRC1 for HBV,Jianyan Yixue(Laboratory Medicine),30(11):1104-1106(宋惠,刘兴晖,沈振华,陈庆,徐凤霞,徐莉敏,2015,胶原三股螺旋重复蛋白1在HBV肝病中的临床意义研究,检验医学,30(11):1104-1106)
Sun L.,Li J.Y.,Duan P.P.,Wu Y.H.,Huang W.J.,and Kong W.M.,2015,The treatment effect of auricular point sticking at different time on promoting the recovery of gastrointestinal function in postoperative patients with gastric cancer,Zhonghua Huli Zazhi(Chinese Journal of Nursing),50(7):844-847(孙龙,李菊云,段培蓓,吴艳红,黄为君,孔维敏,2015,不同时间点耳穴贴压对胃癌患者术后胃肠功能恢复的作用,中华护理杂志,50(7):844-847)
Yasuda K.,Shiraishi N.,Suematsu T.,Yamaguchi K.,Adachi Y.,and Kitano S.,2015,Rate of detection of lymph node metastasis is correlated with the depth of submucosal invasion in early stage gastric carcinoma,Cancer,85(10):2119-2123
Zhou J.,Hiki N.,Mine S.,Kumagai K.,Ida S.,Jiang X.,Nunobe S.,Ohashi M.,Sano T.,and Yamaguchi T.,2016,Role of prealbumin as a powerful and simple index for predicting postoperative complications after gastric cancer surgery,Annals of Surgical Oncology,24(2):510-517
Cristescu R.,Lee J.,Nebozhyn M.,Kim K.M.,Ting J.C.,Wong S.S.,Liu J.,Yue Y.G.,Wang J.,Yu K.,Ye X.S.,Do I.G.,Liu S.,Gong L.,Fu J.,Jin J.G.,Choi M.G.,Sohn T.S.,Lee J.H.,Bae J.M.,Kim S.T.,Park S.H.,Sohn I.,Jung S.H.,Tan P.,Chen R.,Hardwick J.,Kang W.K.,Ayers M.,Hongyue D.,Reinhard C.,Loboda A.,Kim S.,and Aggarwal A.,2015,Molecular analysis of gastric cancer identifies subtypes associated with distinct clinical outcomes,Nature Medicine,21(5):449-456
Graham D.Y.,2015,Helicobacter pylori update:gastric cancer,reliable therapy,and possible benefits,Gastroenterology,148(4):719-731
Guo K.,Zhou M.G.,and Pan X.P.,2015,Trends and characteristics of gastric cancer mortality in China,2006~2012,Zhongguo Gonggong Weisheng(Chinese Journal of Public Health),31(3):341-343(郭康,周脉耕,潘晓平,2015,中国居民2006-2012年胃癌死亡趋势分析,中国公共卫生,31(3):341-343)
Kodera Y.,Isobe K.,Yamauchi M.,Kondoh K.,Kimura N.,Akiyama S.,Itoh K.,Nakashima I.,and Takagi H.,2015,Expression of nm23 H-1 RNA levels in human gastric cancer tissues,a negative correlation with nodal metastasis,Cancer,73(2):259-265
Lang L.,Zhou M.L.,Xu L.Y.,Du Y.E.,Wen S.Y.,and Liu M.R.,2016,TGF-βsignaling pathway regulates the mammosphere formation and migration of highly Twist-expressed breast cancer cells,Zhongliu(Tumor),36(10):1083-1089(郎磊,周明莉,徐丽云,杜燕娥,文思阳,柳满然,2016,TGF-β信号通路调控Twist高表达型乳腺肿瘤细胞微球体的形成及迁移,肿瘤,36(10):1083-1089)
Li Y.X.,Li X.M.,Zhang N.,Zhang W.,Chen C.,Tao L.,Zhao J.,Li S.G.,and Li F.,2015,Associations of Helicobacter pylori infection with the pathogenesis,progression and prognosis in patients with gastric cancer,Zhonghua Zhongliu Yufang Zazhi(Chinese Journal of Cancer Prevention and Treatment),22(2):91-94(李一鑫,李秀明,张楠,张微,陈成,陶林,赵瑾,李述刚,李锋,2015,幽门螺杆菌感染与胃癌发生发展及预后的相关性研究,中华肿瘤防治杂志,22(2):91-94)
Shuai X.M.,Gao J.B.,Liu J.H.,Zhang P.,Bai J.,Cai K.L.,Wang G.B.,and Tao K.X.,2015,Clinical efficacy of neoadjuvant chemotherapy combined with laparoscopy-assisted radical gastrectomy for advanced gastric cancer,Zhonghua Xiaohua Waike Zazhi(Chinese Journal of Digestive Surgery),15(3):241-246(帅晓明,高金波,刘兴华,张鹏,白洁,蔡开琳,王国斌,陶凯雄,2016,新辅助化疗联合腹腔镜辅助胃癌根治术治疗进展期胃癌的疗效分析,中华消化外科杂志,15(3):241-246)
Song H.,Liu X.H.,Shen Z.H.,Chen Q.,Xu F.X.,and Xu L.M.,2015,Research on the clinical significance of CTHRC1 for HBV,Jianyan Yixue(Laboratory Medicine),30(11):1104-1106(宋惠,刘兴晖,沈振华,陈庆,徐凤霞,徐莉敏,2015,胶原三股螺旋重复蛋白1在HBV肝病中的临床意义研究,检验医学,30(11):1104-1106)
Sun L.,Li J.Y.,Duan P.P.,Wu Y.H.,Huang W.J.,and Kong W.M.,2015,The treatment effect of auricular point sticking at different time on promoting the recovery of gastrointestinal function in postoperative patients with gastric cancer,Zhonghua Huli Zazhi(Chinese Journal of Nursing),50(7):844-847(孙龙,李菊云,段培蓓,吴艳红,黄为君,孔维敏,2015,不同时间点耳穴贴压对胃癌患者术后胃肠功能恢复的作用,中华护理杂志,50(7):844-847)
Yasuda K.,Shiraishi N.,Suematsu T.,Yamaguchi K.,Adachi Y.,and Kitano S.,2015,Rate of detection of lymph node metastasis is correlated with the depth of submucosal invasion in early stage gastric carcinoma,Cancer,85(10):2119-2123
Zhou J.,Hiki N.,Mine S.,Kumagai K.,Ida S.,Jiang X.,Nunobe S.,Ohashi M.,Sano T.,and Yamaguchi T.,2016,Role of prealbumin as a powerful and simple index for predicting postoperative complications after gastric cancer surgery,Annals of Surgical Oncology,24(2):510-517