正交实验优化回流法提取五桠果叶木姜子叶总黄酮工艺研究
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摘要
目的:研究回流法提取五桠果叶木姜子叶总黄酮的最佳提取工艺。方法:用回流法提取五桠果叶木姜子叶的总黄酮,以五桠果叶木姜子叶中的总黄酮为指标,以芦丁为对照品,采用硝酸铝显色法,以可见分光光度计测定总黄酮含量;以乙醇浓度、料液比、回流温度、回流时间4个因素进行单因素实验,再用正交实验优化确定最佳提取工艺。结果:回流法提取的最佳工艺为:乙醇浓度40%,料液比1∶30(g/mL),回流温度80℃,回流时间1.5h,在该条件下总黄酮得率最高可达9.55%。结论:采用回流法提取五桠果叶木姜子叶总黄酮,具有可行性。
Objective:The optimum extraction technology of total flavonoids of the Litsea dilleniifolia leaves is studied by using reflux extraction method.Methods:The total flavonoids of Litsea dilleniifolia leaves as index,with rutin as reference substance,using reflux extraction method to extract the total flavonoids from the Litsea dilleniifolia leaves,and with the visible spectrophotometer,using Al(NO3)3 Chromogenic method for the determination of total flavonoids content,using the single factor experiment to test from the concentration of ethanol concentration,solid-liquid ratio,reflux temperature and reflux time these four factors,the optimal extraction technology will be determined by orthogonal experiment.Results:The best condition for the reflux extraction is 40% ethanol,solid-liquid ratio 1:30(g/mL),reflux temperature 80℃,reflux time1.5 h,and the maximum yield can up to 9.55%.Conclusion:It is feasible to extract the total flavonoids from the Litsea dilleniifolia leaves with the reflux extraction method.
Objective:The optimum extraction technology of total flavonoids of the Litsea dilleniifolia leaves is studied by using reflux extraction method.Methods:The total flavonoids of Litsea dilleniifolia leaves as index,with rutin as reference substance,using reflux extraction method to extract the total flavonoids from the Litsea dilleniifolia leaves,and with the visible spectrophotometer,using Al(NO3)3 Chromogenic method for the determination of total flavonoids content,using the single factor experiment to test from the concentration of ethanol concentration,solid-liquid ratio,reflux temperature and reflux time these four factors,the optimal extraction technology will be determined by orthogonal experiment.Results:The best condition for the reflux extraction is 40% ethanol,solid-liquid ratio 1:30(g/mL),reflux temperature 80℃,reflux time1.5 h,and the maximum yield can up to 9.55%.Conclusion:It is feasible to extract the total flavonoids from the Litsea dilleniifolia leaves with the reflux extraction method.
引文
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[7]梁萱,赵建军,梁永锋.金银花中黄酮提取工艺研究及野生与人工种植金银花中黄酮含量的比较[J].中南药学,2013,1(11):173-175.
[2]刘演,宁世江.广西重点保护野生植物资源的现状与评价[J].广西科学,2002,9(2):124-132.
[3]吕仕洪,李先琨,向悟生,等.广西弄岗五桠果叶木姜子群落结构特征与种群动态[J].植物资源与环境学报,2004,13(2):25-30.
[4]丁利君,吴振辉,蔡创海,等.金银花中黄酮物质最佳提取工艺研究[J].食品科学,2002,23(2):62-65.
[5]孙崇鲁,黄克瀛,陈丛瑾,等.香樟叶中黄酮类化合物的提取[J].应用化工,2006,35(2):412-413.
[6]李建北,杨敬芝,丁怡.两种木姜子属植物的化学成分研究[J].中草药,2001,32(7):593-594.
[7]梁萱,赵建军,梁永锋.金银花中黄酮提取工艺研究及野生与人工种植金银花中黄酮含量的比较[J].中南药学,2013,1(11):173-175.