温阳振衰颗粒对心肌细胞损伤模型LncRNABIC的影响
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摘要
目的观察温阳振衰颗粒含药血清对H9C2心肌细胞损伤模型LncRNA BIC表达的影响并探讨其作用机制。方法 H9C2心肌细胞分为正常对照组、正常加含药血清组、阿霉素(ADR)组(ADR组)、ADR加含药血清组。其中ADR组、含药血清组分别为在培养基中加入2.67μmol/L的ADR和加入10%的温阳振衰颗粒含药血清。各组均培养相同时间后检测实验末各组细胞LncRNA BIC的表达水平。结果倒置显微镜下可见正常对照组、正常加含药血清组心肌细胞形态、胞质、间质及横纹均为正常;ADR组心肌细胞出现肌纤维的溶解,横纹模糊,及胞浆空包变性,且表现出不同程度的炎症细胞浸润和间质水肿。ADR加含药血清组的心肌细胞较ADR组,其肌纤维溶解、恒温模糊、胞浆空泡变性、炎症细胞浸润、间质水肿等情况均有所改善。正常对照组、正常加含药血清组的细胞存活率相当(P> 0.05);ADR组、ADR加含药血清组与正常对照组比较,其细胞存活率均明显下降(P <0.05);ADR加含药血清组与ADR组比较,其细胞存活率有所上升(P <0.05)。正常加含药血清组与正常组比较,LncRNA BIC表达明显升高(P <0.05);ADR组与正常组比较,LncRNA BIC表达明显降低(P <0.05);ADR加含药血清组与ADR组比较,LncRNA BIC表达升高(P <0.05)。结论温阳振衰颗粒能上调LncRNA BIC的表达,这可能是其治疗慢性心衰的重要机制之一。
Objective: To observe the effects of Wenyang Zhenshuai Granule containing serum on the expression of LncRNA BIC in H9 C2 myocardial cell injury model. Methods: H9 C2 myocardial cells were divided into the normal control group,normal+drug serum group,ADR group,and ADR+ drug serum group. 2.67 μmol/L ADR and 10% drug serum were added to ADR group and ADR+ drug serum group,respectively. LncRNA BIC expression in these groups were detected by RT-PCR. Results: Compared with the normal control group,the expression of LncRNA BIC was significantly down-regulated in ADR group;the difference was statistically significant(P <0.05). Drug serum group could significantly increase the expression of LncRNA BIC;the difference was statistically significant(P < 0.05). Conclusion: Wenyang Zhenshuai Granule can up-regulate the expression of LncRNA BIC,which may be one of the important mechanisms on chronic heart failure.
Objective: To observe the effects of Wenyang Zhenshuai Granule containing serum on the expression of LncRNA BIC in H9 C2 myocardial cell injury model. Methods: H9 C2 myocardial cells were divided into the normal control group,normal+drug serum group,ADR group,and ADR+ drug serum group. 2.67 μmol/L ADR and 10% drug serum were added to ADR group and ADR+ drug serum group,respectively. LncRNA BIC expression in these groups were detected by RT-PCR. Results: Compared with the normal control group,the expression of LncRNA BIC was significantly down-regulated in ADR group;the difference was statistically significant(P <0.05). Drug serum group could significantly increase the expression of LncRNA BIC;the difference was statistically significant(P < 0.05). Conclusion: Wenyang Zhenshuai Granule can up-regulate the expression of LncRNA BIC,which may be one of the important mechanisms on chronic heart failure.
引文
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[16]雷国奇.四逆汤类方证治规律及临床应用研究[D].武汉:湖北中医药大学,2010.
[2]陈灏珠,林果为.实用内科学[M].北京:人民卫生出版社,2012:1366.
[3]Hunt SA.ACC/AHA 2005 guideline update for the diagnosis and management of chronic heart failure in the adult:a report of the American College Cardiology/America Heart Association task force on practice guidelines[J].Circulation,2005,112(12):154-235.
[4]Chen Xinyu,Tang Yanping,Chen Haiying,et al.Wenyangzhenshuai Granule improves rabbit chronic heart failure induced by adriamycin through JNK/P38 signaling pathway[J].Journal of Computational and Theoretical Nanoscience,2017(14):1-6.
[5]Zhu J,Chen T,Yang L,et al.Regulation of microRNA-155in atherosclerotic inflammatory responses by targeting MAP3K10[J].PLoS One,2012,7(11):51.
[6]Zhong J,Kyriakis JM.Dissection of a signaling pathway by which pathogen-associated molecular patterns recruit the JNK and p38 MAPKs and trigger cytokine release[J].J Biol Chem,2007,282(33):46-54.
[7]Chen Xinyu,Cai Huzhi,Chen Qingyang,et al.Effects of Wenyangzhenshuai Granule on ERK1/2 and ERK5 activity in the myocardial tissue in a rabbit model of adriamycin-induced chronic heart failure[J].International Journal of Clinical and Experimental Medicine,2015,8(11):20732-20741.
[8]何玲,孙桂波,孙潇,等.木樨草苷对阿霉素诱导乳鼠心肌细胞损伤的保护作用[J].中国药理学通报,2012,28(9):1229-1233.
[9]Frantz S,Behr T,Hu K,et al.Role of p38 mito gen-activated protein kinase in cardiac remodeling[J].Br J Pharmacol,2007,150(2):130-135.
[10]Xu Y1,Xiao H1,Luo H,et al.Inhibitory effects of oxymatrine on TGF-β1induced proliferation and abnormal differentiation in rat cardiac fibroblasts via the p38MAPK and ERK1/2signaling pathways[J].Mol Med Rep,2017,16(4):5354-5362.
[11]Kasier RA,Bueno OF,Lips DJ,et al.Targeted inhibition o P38 mitogen-activated protein kinase antagonizes cardiac injury and cell death following ischemia-reperfusion in vivo[J].J Biol Chem,2004,279(5):15524-15530.
[12]Yousif NG,Hadi NR,Al-Amran F,et al.Cardioprotective effects of irbesartan in polymicrobial sepsis:The role of the p38MAPK/NF-κB signaling pathway[J].Herz,2018,43(2):140-145.
[13]Lucas T,Bonauer A,Dimmeler S.RNA therapeutics in cardiovascular disease[J].Circ Res,2018,123(2):205-220.
[14]张智琳,洪永敦.浅析四逆汤类方治疗心力衰竭的特点[J].中华中医药杂志2005,20(4):225-227.
[15]张宏,彭成,余成浩.附子煎煮时间、给药剂量与温阳功效的相关性研究[J].中国中药杂志,2007,32(20):2118-2122.
[16]雷国奇.四逆汤类方证治规律及临床应用研究[D].武汉:湖北中医药大学,2010.