摘要
隐花色素作为植物体内重要的光受体,对植物生长发育的调控过程起着重要的作用。为了研究隐花色素在马铃薯中的调控作用,本研究以马铃薯‘大西洋’为试验材料,采用RT-PCR的方法从叶片中克隆得到1个隐花色素基因Cryptochrome 1,其ORF全长为1 749 bp,编码583个氨基酸,序列比对和结构域分析表明,此蛋白包含1个PHR和1个CCT结构域,将其命名为StCRY1。系统进化树分析表明,StCRY1与番茄CRY1亲缘关系最近。实时荧光定量PCR表明,StCRY1基因在马铃薯的不同组织器官中均有表达,在叶片中的表达量最高。
Cryptochrome, as an im portant light receptor in plants, plays an important role in the regulation of plant growth and development. In order to study the regulation of cryptochrome in potatoes, Cryptochrome 1 gene was cloned from leaves of potato 'Atlantic' by RT-PCR. Its ORF length was 1 749 bp, encoding 583 amino acids.Sequence alignment and domain analysis showed that the protein contained one PHR and one CCT domain, which was named StCRY1. Phylogenetic tree analysis indicated that StCRY1 had the closest relationship with tomato CRY1. Real-time fluorescence quantitative PCR suggested that StCRY1 gene was expressed in different tissues and organs of potato, with the highest expression in leaves.
引文
Bae G.,and Choi G.,2008,Decoding of light signals by plant phytochromes and their infracting proteins,Annu.Rev.Plant Biol.,59:281-311
Barrero J.M.,Downie A.B.,Xu Q.,and Gubler F.,2014,A role for barley CRYPTOCHROME1 in light regulation of grain dormancy and germination,Plant Cell,26(3):1094-1104
Giliberto L.,Perrotta P.,Pallara P.,Weller J.L.,Fraser P.D.,Bramley P.M.,Fiore A.,Tavazza M.,and Giuliano G.,2005,Manipulation of the blue light photoreceptor cryptochrome 2in tomato affects vegetative development,flowering time,and fruit antioxidant content,Plant Physiol.,137(1):199-208
Hirose F.,Shinomura T.,Tanabata T.,Shimada H.,and Takano M.,2006,Involvement of rice cryptochromes in de-etiolation responses and flowering,Plant Cell Physiol.,47(7):915-925
Imaizmni T.,Kanegae T.,and Wada M.,2000,Cryptochrome nucleocytoplasmic distribution and gene expression are regulated by light quality in the fern Adiantum capillus-veneris,Plant Cell,12(1):81-96
Johns T.,ed.,2016,Potato:origins and conservation of potato genetic diversity,Spinger-Verlag,Berlin Heidelberg,Germany,pp.3562-3564
Li J.G.,Li G.,Wang H.Y.,and Deng X.W.,2004,Phytochrome signaling mechanisms,The Arabidopsis Book,3(3):e0148
Li Q.H.,and Yang H.Q.,2007,Cryptochrome signaling in plants,Photochem.Photobiol.,83(1):94-101
Lin C.T.,2002,Blue light receptors and signal transduction,Plant Cell,14(1):207-225
Lin C.T.,and Shalitin D.,2003,Cryptochrome structure and signal transduction,Annu.Rev.Plant Biol.,54(1):469-496
Matsumoto N.,Hirano T.,Iwasaki T.,and Yamamoto N.,2003,Functional analysis and intracellulat localization of rice cryptochromes,Plant Physiol.,133(4):1494-1503
Ninu L.,Ahmad M.,Miarelli C.,Cashmore A.R.,and Giuliano G.,1999,Cryptochrome 1 controls tomato development in response to blue light,Plant Journal,18(5):551-556
Platten J.D.,Foo E.,Elliott R.C.,Hecht V.,Reid J.B.,and Weller J.L.,2005,Cryptochrome 1 contributes to blue-light sensing in pea,Plant Physiol.,139(3):1472-1482
Quail P.H.,2002,Phytochrome photosensory signaling networks,Nat.Rev.Mol.Cell Biol.,3(2):85-93
Xu P.,Xiang Y.,Zhu H.L.,Xu H.B.,Zhang Z.Z.,Zhang C.Q.,Zhang L.X.,and Ma Z.Q.,2009,Wheat cryptochromes:subcrllular localization and involvement in photomorphogenesis and osmotic stress responses,Plant Physiol.,149(2):760-774
Yan L.,Yang Z.J.,Su L.,Xiao Y.,Guo L.,Song M.F.,Sun L.,Meng F.H.,Bai J.R.,and Yang J.P.,2016,Molecular cloning of two maize(Zea mays)CRY1a genes and their expression patterns of in response to different light treatments,Zuowu Xuebao(Acta Agronomica Sinica),42(9):1298-1308(闫蕾,杨宗举,苏亮,肖阳,郭林,宋梅芳,孙蕾,孟凡华,白建荣,杨建平,2016,2个玉米ZmCRY1a基因的克隆及其响应光质处理的表达模式,作物学报,42(9):1298-1308)
Zhan K.H.,Li Z.Y.,Hou P.,Xi Y.L.,Xiao Y.,Meng F.H.,and Yang J.P.,2012,A new strategy for crop improvement through modification of phytochrome signalling pathways,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),45(16):3249-3255(詹克慧,李志勇,侯佩,习雨琳,肖阳,孟凡华,杨建平,2012,利用修饰光敏色素信号途径进行品种改良的可行性,中国农业科学,45(16):3249-3255)
Zhang Q.Z.,Li H.Y.,Li R.,Hu R.B.,Fan C.M.,Chen F.L.,Wang Z.H.,Liu X.,Fu Y.F.,and Lin C.T.,2008,Association of the circadian rhythmic ex-pression of GmCRY1a with a latitudinal cline in photoperiodic flowering of soybean,Proc.Natl.Acad.Sci.USA,105(52):21028-21033
Zhang Y.C.,Gong S.F.,Li Q.H.,Sang Y.,and Yang H.Q.,2006,Functional and signaling mechanism analysis of rice Cryptochrome 1,Plant J.,46(6):971-983
Zhu C.L.,Zhang G.R.,Cai A.J.,and Du J.Y.,2009,Advances in the structure and function of cryptochromen in plant,Genomics and Applied Biology,28(1):174-178