不同品系黄连的核型分析
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摘要
采用压片法对黄连(Coptis chinensis Franch.)不同品系植株的染色体数目进行统计及核型分析。结果显示,黄连3个品系的染色体数目相同,均为2n=18;但核型公式和核型类型有所差异,大花叶黄连和有光叶黄连的核型公式均为2n=18=18m,小花叶黄连核型公式为2n=18=6m+10sm+2tm;大花叶黄连和有光叶黄连核型类型均为1A,小花叶黄连核型类型为3B。黄连不同品系间染色体进化程度有所差异,可以为栽培上筛选良种和遗传育种提供参考。
Chromosome number was calculated and karyotype was analyzed on different varieties of Coptis chinensis using the method of squash. The results show that three varieties of Coptis chinensis have the same number of chromosomes and all are2n=18. Karyotype formula and karyotype type are differences,karyotype formula of Dahuaye and Youguangye are 2n=18=18m,karyotype formula of Xiaohuaye is 2n=18=6m+10sm+2tm. karyotype type of Dahuaye and Youguangye are 1A,karyotype type of Xiaohuaye is 3B. There are several evolution degree differences for different strains of C. chinensis,which can provide some prerequisites for improving selection of variety.
Chromosome number was calculated and karyotype was analyzed on different varieties of Coptis chinensis using the method of squash. The results show that three varieties of Coptis chinensis have the same number of chromosomes and all are2n=18. Karyotype formula and karyotype type are differences,karyotype formula of Dahuaye and Youguangye are 2n=18=18m,karyotype formula of Xiaohuaye is 2n=18=6m+10sm+2tm. karyotype type of Dahuaye and Youguangye are 1A,karyotype type of Xiaohuaye is 3B. There are several evolution degree differences for different strains of C. chinensis,which can provide some prerequisites for improving selection of variety.
引文
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[5]李懋学,张赞平.作物染色体及其研究技术[M].北京:中国农业出版社,1996.220-221.
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[9]WANG X W,CHANG SH J,DILIXIATI,et al.Chromosome numbers and karyotypes of genus Salsola in Xinjiang[J].Acta Bot Boreal Occident Sin,2008,28(1):65-71.
[2]WANG D H.Studies on types of character variation of Coptis chinensis population[J].Chin Tradit Herb Drugs,2002,33(6):558-560.
[3]朱强,王有为,齐海涛,等.不同品系黄连产量和质量的研究[J].中草药,2006,37(12):1866-1869.
[4]WU SH L,BAO W B,SH U Q T,et al.Cluster analysis of resemblance near coefficient and evolutionary distance in Anhui pig species[J].Chinese Journal of Animal Science,2006,42(21):4-6.
[5]李懋学,张赞平.作物染色体及其研究技术[M].北京:中国农业出版社,1996.220-221.
[6]LEVAN A,FREDGE K,SANDBERG A A.Nomenclature for centromeric position on chromosome[J].Hereditas,1964,52(2):201-220.
[7]STEBBINS G L.Chromosomal evolution in high plants[M].London:Edward Arold Ltd,1971.87-90.
[8]KUO S R,WANG T T,HUANG T C.Karyotype analysis of some for mosangy nosperm[J].Taiwania,1972,17(1):66-80.
[9]WANG X W,CHANG SH J,DILIXIATI,et al.Chromosome numbers and karyotypes of genus Salsola in Xinjiang[J].Acta Bot Boreal Occident Sin,2008,28(1):65-71.