内生真菌对营养亏缺下金钗石斛幼苗生长的影响
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摘要
【目的】探讨营养亏缺下内生真菌对金钗石斛组培苗生长的影响。【方法】金钗石斛组培苗移栽时在其根部分别添加内生真菌37号、117号、120号菌株及无菌水,然后再分别在1倍、1/2倍、1/4倍和1/8倍Hoagland营养液中培养30 d,通过测定植株生长特征、生理特性等指标来判定内生真菌在不同稀释倍数营养液中的作用效果。【结果】随着Hoagland营养液逐渐变稀,各内生真菌处理组的植株干重、叶绿素SPAD值、总生物碱、水溶性多糖和氮磷钾含量增加量显著高于对照组,根长和根茎比增加量显著小于对照组。3株内生真菌处理间各种指标变化并不完全相同,37号菌株在增加干重和总生物碱含量上作用最为显著,而120号菌株在控制根长和根茎比、提高水溶性多糖含量、增加对磷和钾元素的吸收上效果更为突出,117号菌株在提高叶绿素SPAD值和促进植株吸收氮元素上作用最为明显。【结论】3株菌株均能促进金钗石斛幼苗在营养亏缺下的生长,但作用效果各不相同,生产中混合使用效果更佳。
[Purpose] The effects of No.37, 117 and 120 endophytic fungi strains isolated from Dendrobium nobile on the growth and physiological characteristics of D. nobile under nutritional deficiency condition were detected in this study.[Method]The endophytic fungi strains No. 37, 117,120 and distilled water were sprayed on the root of tissue culture regenerated seedlings of D. nobile.Each group was cultivated for 30 days with 1, 1/2, 1/4 and 1/8 of Hoagland nutrient solution, respectively. After 30 days, the effect of endophytic fungi inoculation was determined by measuring the plant growth and physiological characteristics.[Results]With the decrease of Hoagland solution concentration, the endophyte treatment groups were significantly higher than the control group(treated with distilled water) in dry weight, chlorophyll SPAD value, total alkaloids, water-soluble polysaccharides and NPK content. The root length and root shoot ratio increased less than the control group. The changes of various indexes were not completely the same between the three endophytic fungi strain treatments. Strain No.37 treatment showed the most significant ability in dry weight and total alkaloid content increase. Strain No.120 was more prominent in controlling root length and root shoot ratio,promoting water-soluble polysaccharide content and phosphorus and potassium absorption. Strain No.117 showed the most obvious effect on increasing chlorophyll SPAD value and promoting nitrogen absorption.[Conclusion]All of these three endophytic fungi strains can promote the growth of D. nobile seedlings under nutrient deficit condition, but they showed different types of effect. Therefore, the combination use of these three endophytic fungi will produce better results.
[Purpose] The effects of No.37, 117 and 120 endophytic fungi strains isolated from Dendrobium nobile on the growth and physiological characteristics of D. nobile under nutritional deficiency condition were detected in this study.[Method]The endophytic fungi strains No. 37, 117,120 and distilled water were sprayed on the root of tissue culture regenerated seedlings of D. nobile.Each group was cultivated for 30 days with 1, 1/2, 1/4 and 1/8 of Hoagland nutrient solution, respectively. After 30 days, the effect of endophytic fungi inoculation was determined by measuring the plant growth and physiological characteristics.[Results]With the decrease of Hoagland solution concentration, the endophyte treatment groups were significantly higher than the control group(treated with distilled water) in dry weight, chlorophyll SPAD value, total alkaloids, water-soluble polysaccharides and NPK content. The root length and root shoot ratio increased less than the control group. The changes of various indexes were not completely the same between the three endophytic fungi strain treatments. Strain No.37 treatment showed the most significant ability in dry weight and total alkaloid content increase. Strain No.120 was more prominent in controlling root length and root shoot ratio,promoting water-soluble polysaccharide content and phosphorus and potassium absorption. Strain No.117 showed the most obvious effect on increasing chlorophyll SPAD value and promoting nitrogen absorption.[Conclusion]All of these three endophytic fungi strains can promote the growth of D. nobile seedlings under nutrient deficit condition, but they showed different types of effect. Therefore, the combination use of these three endophytic fungi will produce better results.
引文
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[22]陈连庆,王小明,裴致达.石斛菌根化组培苗对N素的吸收利用[J].林业科学,2007,43(1):48.
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[24]陈连庆,王小明,裴致达.石斛菌根化组培苗对P素吸收利用的研究[J].林业科学研究,2005,18(2):163.DOI:10.13275/j.cnki.lykxyj.2005.02.011.
[25]杨建文,凌鸿,张盈,等.内生真菌对兰科药用植物种子萌发作用研究进展[J].菌物学报,2018,37(1):22.DOI:10.13346/j.mycosystema.170198.
[26]JOHRI A K,OELMüLLER R,DUA M,et al.Fungal association and utilization of phosphate by plants:success,limitations,and future prospects[J].Frontiers in Microbiology,2015,6(3):984.DOI:10.3389/fmicb.2015.00984.
[27]王秋霞,严宁,纪大干.菌根真菌促进金钗石斛的生长及氮利用[J].植物分类与资源学报,2014,36(3):321.
[2]付芳婧,刘政.金钗石斛优良种源的适生条件及仿野生栽培的关键技术[J].种子,2012,31(7):137.DOI:10.16590/j.cnki.1001-4705.2012.07.078.
[3]梁洁,滕建北,劳文然.不同栽培方法和生长时间的美花石斛中多糖含量的比较[J].华西药学杂志,2010,25(1):063.
[4]江爱明,曹俊,蔡高磊.秦巴山区石斛属亲缘关系及金钗石斛遗传多样性ISSR分析[J].西北植物学报,2016,36(10):1977.
[5]唐德英,李荣英,李学兰,等.金钗石斛试管苗仿野生栽培技术研究[J].中国中药杂志,2008,33(10):1208.
[6]王再花,朱根发,操君喜,等.不同N、P、K水平施肥对春石斛营养生长和开花的影响[J].中国农学通报,2011,27(16):248.
[7]罗剑飘,谭嘉娜,杨俊贤,等.不同氮磷钾营养水平对铁皮石斛组培苗生长的影响[J].南方农业学报,2017,48(3):481.
[8]徐焰平.铁皮石斛内生真菌研究进展[J].微生物学杂志,2015,35(5):108.
[9]黄晖,邵士成,高江云.不同内生真菌对齿瓣石斛幼苗生长的效应[J].中国中药杂志,2016,41(11):2019.
[10]谢玲,张雯龙,蓝桃菊,等.1株内生真菌的分离鉴定及其对铁皮石斛的促生作用[J].华中农业大学学报,2016,35(3):83.
[11]朱波,刘京晶,斯金平,等.铁皮石斛内生真菌对宿主组培苗生长与代谢成分的影响[J].中国中药杂志,2016,41(9):1602.
[12]梁宇,高玉葆.内生真菌对植物生长发育及抗逆性的影响[J].植物学通报,2000,17(1):52.
[13]刘润进,唐明,陈应龙.菌根真菌与植物抗逆性研究进展[J].菌物研究,2017,15(1):70.
[14]龚记熠,刘杰,李菲,等.内生真菌对干旱胁迫下金钗石斛生理生化特性的影响[J].安徽农业大学学报,2018,45(1):156.DOI:10.13610/j.cnki.1672-352x.20180302.001.
[15]谢安强,洪伟,吴承祯,等.内生真菌对低磷胁迫下尾巨桉生理及土壤特性的影响[J].西南林业大学学报,2013,33(3):1.
[16]LLEDóS,RODRIGO S,POBLACIONES M J,et al.Biomass yield,mineral content,and nutritive value of Poa pratensis as affected by non-clavicipitaceous fungal endophytes[J].Mycological Progress,2015,14(9):67.DOI:10.1007/s11557-015-1093-4.
[17]GOSAL S K,BADDESHA H S,KALIA A,et al.Role of endophytic fungus Piriformospora indica in nutrient acquisition and improving grain yield of maize in phosphorus deficient soil under field conditions[J].Environment and Ecology,2008,26(4b):2172.
[18]刘莉,钱均祥,萧凤回,等.快速测定铁皮石斛总生物碱含量方法的优化[J].西南农业学报,2015,28(2):575.DOI:10.16213/j.cnki.scjas.2015.02.023.
[19]中科院植生所上海市植物生理学会.现代植物生理学实验指南[M].北京:科学出版社,l999.
[20]赵杨景,郭顺星,高薇薇,等.三种内生真菌与大花蕙兰共生对矿质元素营养吸收的影响[J].园艺学报,1999,26(2):110.
[21]闫浩利,陈晓梅,廖方华,等.菌根真菌对金钗石斛幼苗生长及石斛碱和多糖积累的影响[J].中国药学杂志,2016,51(17):1450.
[22]陈连庆,王小明,裴致达.石斛菌根化组培苗对N素的吸收利用[J].林业科学,2007,43(1):48.
[23]ALEXANDER C,ALEXANDER I J,HADLEY G.Phosphate uptake by Goodyera repens in relation to mycorrhizal infection[J].New Phytologist,1984,97(3):401.DOI:10.1111/j.1469-8137.1984.tb03606.x.
[24]陈连庆,王小明,裴致达.石斛菌根化组培苗对P素吸收利用的研究[J].林业科学研究,2005,18(2):163.DOI:10.13275/j.cnki.lykxyj.2005.02.011.
[25]杨建文,凌鸿,张盈,等.内生真菌对兰科药用植物种子萌发作用研究进展[J].菌物学报,2018,37(1):22.DOI:10.13346/j.mycosystema.170198.
[26]JOHRI A K,OELMüLLER R,DUA M,et al.Fungal association and utilization of phosphate by plants:success,limitations,and future prospects[J].Frontiers in Microbiology,2015,6(3):984.DOI:10.3389/fmicb.2015.00984.
[27]王秋霞,严宁,纪大干.菌根真菌促进金钗石斛的生长及氮利用[J].植物分类与资源学报,2014,36(3):321.