基于激光荧光光谱技术的儿童肿瘤早期诊断
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摘要
传统儿童肿瘤早期诊断方法未对激光荧光特征光谱进行分析,有效性差,为此提出基于激光荧光光谱技术的儿童肿瘤早期诊断方法,采用可调谐激光器发出激光耦合光纤束,照射到人体组织上产生自体荧光,荧光信息传送到计算机进行解析,为避免远紫外光对儿童皮肤的损害,选择365 nm～415 nm紫外波段的激光激发自体荧光。通过激光诱导荧光特征光谱分离算法计算RBFN权值,将激光诱导荧光特征光谱中的各个光谱变量分离出来,利用特征光谱分析算法获得各个光谱变量的值,分析这些光谱变量值和人体组织液中的荧光因子实现儿童肿瘤的早期诊断。实验结果表明,本文方法可实现儿童肿瘤早期精确诊断。
The early diagnosis method of childhood tumors based on confocal laser microscope and digestive tract was not analyzed and the effectiveness was poor. An early diagnosis method for childhood tumors based on laser fluorescence spectroscopy is proposed. A laser coupled fiber beam is emitted by a tunable laser,which irradiates into human tissues to produce an autologous fluorescence,and fluorescence information is transmitted to the computer for analysis.In order to avoid the damage of ultraviolet light to the skin of children,laser excitation self-fluorescence in the ultraviolet band of 365 nm to 415 nm was selected. The weight of RBFN is calculated by the laser induced fluorescence characteristic spectral separation algorithm. Each spectral variable in the laser induced fluorescence characteristic spectrum is separated and the value of each spectral variable is obtained by using the characteristic spectral analysis algorithm.These spectral variables and fluorescence factors in human tissue fluid are analyzed to realize early diagnosis of childhood tumors. The experimental results show that the concentration of humic acid solution of 3. 9 mg/l obtained by the proposed method is very close to the actual concentration of 4 mg/l,indicating that the proposed method can accurately detect abnormal tissue fluids in children. At the same time,the best excitation wavelength of 380 nm with the maximum fluorescence difference can be used for early diagnosis of childhood tumors.
The early diagnosis method of childhood tumors based on confocal laser microscope and digestive tract was not analyzed and the effectiveness was poor. An early diagnosis method for childhood tumors based on laser fluorescence spectroscopy is proposed. A laser coupled fiber beam is emitted by a tunable laser,which irradiates into human tissues to produce an autologous fluorescence,and fluorescence information is transmitted to the computer for analysis.In order to avoid the damage of ultraviolet light to the skin of children,laser excitation self-fluorescence in the ultraviolet band of 365 nm to 415 nm was selected. The weight of RBFN is calculated by the laser induced fluorescence characteristic spectral separation algorithm. Each spectral variable in the laser induced fluorescence characteristic spectrum is separated and the value of each spectral variable is obtained by using the characteristic spectral analysis algorithm.These spectral variables and fluorescence factors in human tissue fluid are analyzed to realize early diagnosis of childhood tumors. The experimental results show that the concentration of humic acid solution of 3. 9 mg/l obtained by the proposed method is very close to the actual concentration of 4 mg/l,indicating that the proposed method can accurately detect abnormal tissue fluids in children. At the same time,the best excitation wavelength of 380 nm with the maximum fluorescence difference can be used for early diagnosis of childhood tumors.
引文
[1]李真,李延青.共聚焦激光显微内镜与消化道早期肿瘤的诊断[J].中国实用内科杂志,2015,35(3):183-186.
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[5]吴淑群,董熙,裴学凯,等.基于激光诱导荧光法诊断大气压低温等离子体射流中OH自由基和O原子的时空分布[J].电工技术学报,2017,32(8):82-94.
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[7]李杰,李晓龙,唐秋华,等.基于PCA的时间分辨油荧光光谱分析及优化[J].光学精密工程,2017,25(4):884-890.
[8]何晓晓,陈素叶,陈冕,等.基于Poly(A)/二氧化硅纳米颗粒的p H可控释放抗肿瘤药物载体[J].科学通报,2014,59(2):181-187.
[9]孙枝红,周理华,邓冠军,等.肿瘤靶向的磁性荧光IR780-Fe3O4纳米颗粒用于循环肿瘤细胞的检测[J].分析化学,2017,45(10):1427-1433.
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[11]杨辉,赵雪松,孙彦飞,等.荧光偏振短距激光雷达测量生物战剂/气溶胶[J].红外与激光工程,2017,004(10):143-150.
[12]贺振武,杨阳,张毅然,等.层流预混乙烷和丙烯火焰中PAHs荧光光谱分析[J].中国电机工程学报,2016,36(16):4478-4483.
[13]郝海燕,刘振,祖莉莉.266nm激光光解C2H5SSC2H5产物的激光诱导荧光光谱[J].物理化学学报,2015,31(11):2029-2035.
[14]刘训臣,李玉阳,周忠岳,等.光谱法和取样分析法在燃烧诊断研究中的应用[J].实验流体力学,2016,30(1):43-54.
[15]饶志敏,华灯鑫,何延尧,等.基于本征荧光的生物气溶胶测量激光雷达性能[J].物理学报,2016,65(20):32-39.
[16]杨珍明,沈磊.蓝激光成像结合放大内镜对早期胃癌及癌前病变的诊断价值[J].中华消化内镜杂志,2017,34(1):24-29.
[17]郭立华,赵伟,张俊杰,等.儿童肝母细胞瘤潜在标志物载脂蛋白A-I的筛选及鉴定[J].中国当代儿科杂志,2016,18(12):1205-1210.
[18]马天加,李俊甲,姜兆群,等.NBI辅助钬激光治疗非肌层浸润性膀胱尿路上皮癌的肿瘤残留率分析[J].中华医学杂志,2015,95(34):2775-2778.
[2]刘晓华,陈思颖,张寅超,等.基于激光诱导荧光的常见机油快速识别方法[J].光谱学与光谱分析,2014,34(8):2148-2151.
[3]张元志,潘亮,王贻坤,等.组织荧光光谱检测系统校准方法研究[J].激光与光电子学进展,2015,52(8):234-240.
[4]吴珍珍,姚鹏,苏荣国,等.基于交替三线性分解的浮游藻荧光识别分析技术研究[J].中国激光,2015,42(5):295-303.
[5]吴淑群,董熙,裴学凯,等.基于激光诱导荧光法诊断大气压低温等离子体射流中OH自由基和O原子的时空分布[J].电工技术学报,2017,32(8):82-94.
[6]方波浪,胡志云,陶波,等.非线性双线原子荧光技术的标定新方法[J].光学学报,2017,37(11):140-146.
[7]李杰,李晓龙,唐秋华,等.基于PCA的时间分辨油荧光光谱分析及优化[J].光学精密工程,2017,25(4):884-890.
[8]何晓晓,陈素叶,陈冕,等.基于Poly(A)/二氧化硅纳米颗粒的p H可控释放抗肿瘤药物载体[J].科学通报,2014,59(2):181-187.
[9]孙枝红,周理华,邓冠军,等.肿瘤靶向的磁性荧光IR780-Fe3O4纳米颗粒用于循环肿瘤细胞的检测[J].分析化学,2017,45(10):1427-1433.
[10]RANULFI A C,CARDINALI M C B,KUBOTA T M K,et al.Laser-induced fluorescence spectroscopy applied to early diagnosis of citrus Huanglongbing[J].Biosystems Engineering,2016,144:133-144.
[11]杨辉,赵雪松,孙彦飞,等.荧光偏振短距激光雷达测量生物战剂/气溶胶[J].红外与激光工程,2017,004(10):143-150.
[12]贺振武,杨阳,张毅然,等.层流预混乙烷和丙烯火焰中PAHs荧光光谱分析[J].中国电机工程学报,2016,36(16):4478-4483.
[13]郝海燕,刘振,祖莉莉.266nm激光光解C2H5SSC2H5产物的激光诱导荧光光谱[J].物理化学学报,2015,31(11):2029-2035.
[14]刘训臣,李玉阳,周忠岳,等.光谱法和取样分析法在燃烧诊断研究中的应用[J].实验流体力学,2016,30(1):43-54.
[15]饶志敏,华灯鑫,何延尧,等.基于本征荧光的生物气溶胶测量激光雷达性能[J].物理学报,2016,65(20):32-39.
[16]杨珍明,沈磊.蓝激光成像结合放大内镜对早期胃癌及癌前病变的诊断价值[J].中华消化内镜杂志,2017,34(1):24-29.
[17]郭立华,赵伟,张俊杰,等.儿童肝母细胞瘤潜在标志物载脂蛋白A-I的筛选及鉴定[J].中国当代儿科杂志,2016,18(12):1205-1210.
[18]马天加,李俊甲,姜兆群,等.NBI辅助钬激光治疗非肌层浸润性膀胱尿路上皮癌的肿瘤残留率分析[J].中华医学杂志,2015,95(34):2775-2778.