补肾和脉方对自发性高血压大鼠肠道微生物组的影响
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摘要
目的观察补肾和脉方对自发性高血压大鼠(SHR)降压疗效及肠道微生物组的影响。方法选择13只雄性SHR,随机分为模型组和补肾和脉方组,灌胃给药8周。每周测量血压,观察结束后取大鼠应激粪便,进行16S V4区扩增子信息采集与分析。结果补肾和脉方对清醒SHR具有明显的降压作用,且用药后补肾和脉方组大鼠肠道微生物组与SHR比较,差异有统计学意义(P<0.05)。结论补肾和脉方具有明确的降压作用,并对大鼠肠道微生物组产生一定影响。
Objective To observe the effect of Bushen Hemai Fang on blood pressure and intestinal microbiome in spontaneous hypertensive rats(SHR).Methods Thirteen male SHRs were randomly divided to the model group and Bushen Hemai Fang group.All of them were orally administered with drugs for 8 weeks and measured blood pressure weekly.We used 16S rDNA V4 amplicon sequencing to analyse the bacteria of the SHR′s stress stool.Results Bushen Hemai Fang had the effect in lowering blood pressure in conscious SHRs.There were significant differences in intestinal microbiome between the two groups after treatment.Conclusion Bushen Hemai Fang has the significant effect in lowering blood pressure and intestinal microbiome in SHRs.
Objective To observe the effect of Bushen Hemai Fang on blood pressure and intestinal microbiome in spontaneous hypertensive rats(SHR).Methods Thirteen male SHRs were randomly divided to the model group and Bushen Hemai Fang group.All of them were orally administered with drugs for 8 weeks and measured blood pressure weekly.We used 16S rDNA V4 amplicon sequencing to analyse the bacteria of the SHR′s stress stool.Results Bushen Hemai Fang had the effect in lowering blood pressure in conscious SHRs.There were significant differences in intestinal microbiome between the two groups after treatment.Conclusion Bushen Hemai Fang has the significant effect in lowering blood pressure and intestinal microbiome in SHRs.
引文
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[20] 金华,金钊,张蕾蕾.肠道菌群可能为高血压发病的环境因素[J].中国微生态学杂志,2015,27(1):121-124.
[21] 吴园琳,余国友,卢雯雯.中药复方对肠道微生态的调节作用研究现状[J].中国中药杂志,2015,40(18):3534-3537.
[2] SANTISTEBAN M M,KIM S,PEPINE C J,et al.Brain-gut-bone marrow axis:implications for hypertension and related therapeutics[J].Circulation Research,2016,118(8):1327-1336.
[3] AGUILAR A.Hypertension:microbiota under pressure[J].Nature Reviews Nephrology,2017,13(1):3.
[4] F?NDRIKS L.Roles of the gut in the metabolic syndrome:an overview[J].Journal of Internal Medicine,2017,281(4):319-336.
[5] 周玉虹,陈宇峰,党大胜,等.肠道菌群对高血压的作用[J].实用药物与临床,2017(3):353-357.
[6] WANG J J,HUANG T,ZHANG C H,et al.Modulation of gut microbiota during probiotic-mediated attenuation of metabolic syndrome in high fat diet-fed mice[J].ISME Journal,2015,9(1):1-15.
[7] PEDERSEN H K,GUDMUNDSDOTTIN V,NIELSEN H B,et al.Human gut microbes impact host serum metabolome and insulin sensitivity[J].Nature,2016,535(7612):376-381.
[8] RICHARDS E M,PEPINE C J,RAIZADA M K,et al.The gut,its microbiome,and hypertension[J].Curr Hypertens Rep,2017,19(4):36.
[9] AFSAR B,VAZIRI N D,ASLAN G,et al.Gut hormones and gut microbiota:implications for kidney function and hypertension[J].JASH,2016,10(12):954-961.
[10] UFNAL M,JAZWIEC R,DADLEZ M,et al.Trimethylamine-N-Oxide:a carnitine-derived metabolite that prolongs the hypertensive effect of angiotensin Ⅱ in rats[J].Can J Cardiol,2014,30(12):1700-1705.
[11] 杨宝,黄丽娜,杨传华.补肾和脉法论治老老年高血压[J].中西医结合心脑血管病杂志,2017,15(14):1803-1805.
[12] 魏建梁,戴洪,陈兴娟,等.补肾和脉法治疗老年单纯收缩期高血压体会[J].中医杂志,2014(20):1786-1787.
[13] 陈兴娟,冯继康,曹新冉,等.补肾和脉方对ApoE-/-小鼠动脉粥样硬化斑块P-选择素和VCAM-1表达的影响[J].山东中医杂志,2015,34(6):456-458.
[14] 杨传华,陆峰,王震,等.补肾和脉方对老年单纯收缩期高血压左室向心性肥厚的影响[J].新中医,2013,45(12):29-31.
[15] 牛文汇.补肾和脉方治疗社区高血压的疗效评价:动态血压研究[D].济南:山东中医药大学,2017.
[16] 宫苏娜.补肾和脉方治疗社区高血压的疗效评价:诊室血压研究[D].济南:山东中医药大学,2017.
[17] 王震.补肾和脉颗粒干预高龄SHR肾损害的作用机制研究[D].济南:山东中医药大学,2017.
[18] YANG T,SANTISTEBAN M M,RODRIGUEZ V,et al.Gut dysbiosis is linked to hypertension[J].Hypertension,2015,65(6):1331-1340.
[19] SANTISTEBAN M M,QI Y,ZUBCEVIC J,et al.Hypertension-linked pathophysiological alterations in the gut[J].Circulation Research,2017,120:312-323.
[20] 金华,金钊,张蕾蕾.肠道菌群可能为高血压发病的环境因素[J].中国微生态学杂志,2015,27(1):121-124.
[21] 吴园琳,余国友,卢雯雯.中药复方对肠道微生态的调节作用研究现状[J].中国中药杂志,2015,40(18):3534-3537.