俄罗斯松针油与加拿大冷杉针油挥发性成分比较与分析
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摘要
为了研究俄罗斯松针油和加拿大冷杉针油的挥发性成分并对比其差异;通过水蒸气蒸馏法提取两种针叶油,并以GC-MS方法结合保留指数对俄罗斯松针油和加拿大冷杉针油挥发成分进行鉴定和定量分析;其中俄罗斯松针油中检测出31种化合物,主要成分包括乙酸龙脑酯(40.158%),δ-4-蒈烯(20.136%),柠檬烯(12.109%),β-蒎烯(5.658%),龙脑(3.864%),萜品油烯(2.594%),β-石竹烯(2.303%),间伞花烃(1.578%),月桂烯(1.212%),α-蛇麻烯(1.037)等;加拿大冷杉针油中检测出47种化合物,主要成分包括β-蒎烯(32.308%),乙酸龙脑酯(17.007%),δ-4-蒈烯(16.663%),柠檬烯(9.711%),β-水芹烯(2.955%),月桂烯(1.807%),α-松油醇(1.666%)等,其中俄罗斯松针油和加拿大冷杉针油共有21个化合物,但成分及含量存在一定差异。研究结果为俄罗斯松针油和加拿大冷杉针油的应用提供理论指导。
This study is to compare the difference of the volatile components of Russian pine needle oil and Canadian fir needle oil;extract two kinds of coniferous essential oil by steam distillation,and the volatile components of Russian pine needle oil and Canadian fir needle oil were identified and quantified by retention index and GC-MS method. 31 compounds were detected in Russian pine needle essential oils,including :borneol acetate(40.158%),δ-4-decene(20.136%),limonene(12.109%),β-terpene(5.658%),borneol(3.864%),terpinolene(2.594%),beta-caryophyllene(2.303%),cymene(1.578%),myrcene(1.212%),alpha-hopsene(1.037),etc. 47 compounds were detected in Canadian fir needle oil,the main components including β-pinene(32.308%),borneol acetate(17.007%),δ-4-decene(16.663%),limonene(9.711%),β-Hydrocelene(2.955%),myrcene(1.807%),α-terpineol(1.666%),etc. Among them,there are 21 compounds in common,but the composition and content are different. The results provide theoretical guidance for the application of Russian pine needle oil and Canadian fir needle oil.
This study is to compare the difference of the volatile components of Russian pine needle oil and Canadian fir needle oil;extract two kinds of coniferous essential oil by steam distillation,and the volatile components of Russian pine needle oil and Canadian fir needle oil were identified and quantified by retention index and GC-MS method. 31 compounds were detected in Russian pine needle essential oils,including :borneol acetate(40.158%),δ-4-decene(20.136%),limonene(12.109%),β-terpene(5.658%),borneol(3.864%),terpinolene(2.594%),beta-caryophyllene(2.303%),cymene(1.578%),myrcene(1.212%),alpha-hopsene(1.037),etc. 47 compounds were detected in Canadian fir needle oil,the main components including β-pinene(32.308%),borneol acetate(17.007%),δ-4-decene(16.663%),limonene(9.711%),β-Hydrocelene(2.955%),myrcene(1.807%),α-terpineol(1.666%),etc. Among them,there are 21 compounds in common,but the composition and content are different. The results provide theoretical guidance for the application of Russian pine needle oil and Canadian fir needle oil.
引文
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[12]刘力恒,王立升,冯丹丹,等.马尾松和湿地松松针挥发性成分的提取及GC-MS比较分析[J].分析试验室,2008,27(11):75-80.
[13]田玉红,李梓,梁才.拉雅松和细叶云南松松针挥发油的化学成分[J].中国实验方剂学杂志,2012,18(1):51-55.
[14]薄采颖,郑光耀,宋强.马尾松、樟子松、臭冷杉针叶精油的化学成分比较研究[J].林产化学与工业,2010,30(6):45-50.
[15]宋平,宋元德.不同方法提取沙田柚柚皮精油成分的研究[J].保鲜与加工,2014(1):41-46.
[16]Leffingwell.ESO 2006[DB/CD].Georgia:Bolens Aroma Chemical Information Service,2010.
[17]胡文杰,罗辉,邹林海,等.雪松松针挥发油化学成分及抗氧化活性研究[J].植物研究,2017,37(4):621-627.
[18]徐丽珊,张姚杰,林颖,等.黄山松松针挥发油提取、GC-MS分析及与湿地松挥发油的比较[J].浙江师范大学学报(自然科学版),2016,39(2):187-192.
[19]李源栋,刘秀明,夏建军,等.GC/MS法结合保留指数分析留兰香油成分[J].中国测试,2016,42(4):45-48.
[2]刘晓庚,陈梅梅.我国松针的开发利用研究及进展[J].生物质化学工程,2003,37(4):29-34.
[3]桑育黎,张芸嘉,高天祥,等.松针挥发油研究进展[J].辽宁大学学报(自然科学版),2018(2):162-169.
[4]文福姬,李健秀,王文涛.松针精油的脱臭特性研究[J].环境科学与技术,2009,32(1):68-70.
[5]魏凤香,李美玉,宋宇宏,等.松针油诱导肝癌HepG2细胞凋亡及对端粒酶活性的影响[J].中药材,2008,32(8):1197-1200.
[6]Kim Y S,Shin D H.Volatile components and antibacterial effects of pine needle(Pinusdensiflora S.and Z.)extracts.[J].Food Microbiology,2005,22(1):37-45.
[7]Barnola L F,Cede?o A,Hasegawa M.Intraindividual variations of volatile terpene contents in Pinuscaribaea needles and its possible relationship to Atta laevigataherbivory[J].Biochemical Systematics&Ecology,1997,25(8):707-716.
[8]Felipe B L,Cede?o A.Inter-population differences in the essential oils of Pinuscaribaea needles.[J].Biochemical Systematics&Ecology,2000,28(10):923-931.
[9]Roussis V,Papadogianni K,Vagias C,et al.Volatile constituents of three Pinus species grown in Greece.[J].Journal of Essential Oil Research,2001,13(2):118-121.
[10]Kurose K,Okamura D,Yatagai M.Composition of the essential oils from the leaves of nine Pinus species and the cones of three of Pinus species[J].Flavour&Fragrance Journal,2010,22(1):10-20.
[11]陈霞,陈辉,高锦明.秦岭油松针叶挥发性物质的成分分析[J].西北植物学报,2005,25(6):1230-1233.
[12]刘力恒,王立升,冯丹丹,等.马尾松和湿地松松针挥发性成分的提取及GC-MS比较分析[J].分析试验室,2008,27(11):75-80.
[13]田玉红,李梓,梁才.拉雅松和细叶云南松松针挥发油的化学成分[J].中国实验方剂学杂志,2012,18(1):51-55.
[14]薄采颖,郑光耀,宋强.马尾松、樟子松、臭冷杉针叶精油的化学成分比较研究[J].林产化学与工业,2010,30(6):45-50.
[15]宋平,宋元德.不同方法提取沙田柚柚皮精油成分的研究[J].保鲜与加工,2014(1):41-46.
[16]Leffingwell.ESO 2006[DB/CD].Georgia:Bolens Aroma Chemical Information Service,2010.
[17]胡文杰,罗辉,邹林海,等.雪松松针挥发油化学成分及抗氧化活性研究[J].植物研究,2017,37(4):621-627.
[18]徐丽珊,张姚杰,林颖,等.黄山松松针挥发油提取、GC-MS分析及与湿地松挥发油的比较[J].浙江师范大学学报(自然科学版),2016,39(2):187-192.
[19]李源栋,刘秀明,夏建军,等.GC/MS法结合保留指数分析留兰香油成分[J].中国测试,2016,42(4):45-48.